Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   sS8_RS01440 Genome accession   NZ_AP017928
Coordinates   304315..305436 (-) Length   373 a.a.
NCBI ID   WP_119628095.1    Uniprot ID   A0A286P3P8
Organism   Methylocaldum marinum strain S8     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 299315..310436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  sS8_RS01420 (sS8_0298) tsaE 299484..299939 (+) 456 WP_119628091.1 tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex ATPase subunit type 1 TsaE -
  sS8_RS01425 (sS8_0299) - 300009..301337 (+) 1329 WP_119628092.1 N-acetylmuramoyl-L-alanine amidase -
  sS8_RS01430 (sS8_0300) mutL 301490..303277 (+) 1788 WP_119628093.1 DNA mismatch repair endonuclease MutL -
  sS8_RS01435 (sS8_0301) miaA 303310..304218 (+) 909 WP_232020694.1 tRNA (adenosine(37)-N6)-dimethylallyltransferase MiaA -
  sS8_RS01440 (sS8_0302) pilU 304315..305436 (-) 1122 WP_119628095.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  sS8_RS01445 (sS8_0303) pilT 305454..306488 (-) 1035 WP_119628096.1 type IV pilus twitching motility protein PilT Machinery gene
  sS8_RS01450 (sS8_0304) - 306577..307302 (+) 726 WP_119628097.1 YggS family pyridoxal phosphate-dependent enzyme -
  sS8_RS01455 (sS8_0305) proC 307390..308214 (+) 825 WP_119628098.1 pyrroline-5-carboxylate reductase -
  sS8_RS01460 (sS8_0306) - 308217..308792 (+) 576 WP_119628099.1 YggT family protein -
  sS8_RS01465 (sS8_0307) - 308794..309093 (+) 300 WP_119628100.1 DUF167 family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41821.99 Da        Isoelectric Point: 6.4330

>NTDB_id=68515 sS8_RS01440 WP_119628095.1 304315..305436(-) (pilU) [Methylocaldum marinum strain S8]
MDFESLLMLMTQKRASDLFITTNKEPCMKIDGVIQPIANAKLSKEQARQIVLGVMNQRQRDEFENTKECNFAISSPGLGR
FRVSAFVQRDTAGMVLRRIETNIPTVDDLNLPPILNELSMTKRGLILFVGATGTGKSTSLAAMIKYRSENSSGHIITIED
PLEYVHPHAGCIITQREVGIDTESYEVALKNTLRQAPDVILIGEIRTRETMQHAITFAETGHLCLSTLHANNANQALDRI
LHFFPEDMHPQLFMDLSLNLRGIIAQQLIRRADGTGRYPAVEILLNTPLASDLIRKGEVHKLKELMKHSREQGMQTFDQA
LYGLYKTGKITYEDALHAADSKNEVRLMIKLGEEGSIEKYAGRNDTLSVVEDD

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=68515 sS8_RS01440 WP_119628095.1 304315..305436(-) (pilU) [Methylocaldum marinum strain S8]
GTGGATTTCGAATCCTTGCTCATGCTGATGACGCAAAAACGAGCGTCCGACCTCTTCATCACCACCAATAAGGAACCGTG
CATGAAGATCGACGGCGTCATTCAACCGATTGCCAATGCCAAGCTCAGCAAGGAACAGGCGCGGCAAATTGTTCTCGGGG
TCATGAATCAACGCCAGCGCGATGAATTCGAGAATACCAAGGAGTGCAATTTCGCGATCTCGTCGCCGGGACTGGGCCGC
TTCCGGGTCAGTGCCTTCGTCCAGAGGGACACCGCCGGAATGGTGCTGAGGCGCATCGAAACCAACATTCCGACCGTGGA
CGATCTCAATCTGCCGCCCATCCTCAATGAACTATCGATGACCAAGCGCGGCCTGATTCTGTTCGTCGGCGCCACCGGAA
CCGGTAAATCCACCTCGCTGGCAGCCATGATCAAATATCGAAGCGAGAATTCGAGCGGCCATATCATCACGATCGAAGAC
CCGCTGGAGTATGTGCATCCGCATGCGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGAATCGTACGAGGT
GGCGCTCAAGAACACCTTGCGGCAGGCGCCCGACGTGATTCTGATCGGGGAAATCCGGACTCGGGAGACGATGCAGCATG
CCATCACCTTCGCCGAGACCGGCCATCTTTGCCTCAGCACGCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGAATT
CTCCACTTTTTCCCCGAAGACATGCACCCGCAGCTGTTCATGGACCTGTCCTTGAACCTGCGGGGAATCATCGCGCAGCA
ACTCATTCGCCGAGCCGACGGCACGGGACGGTATCCGGCCGTCGAAATTCTTCTGAACACCCCTCTGGCTTCCGACCTCA
TCCGCAAAGGCGAGGTCCATAAGCTGAAGGAACTCATGAAGCATTCGCGGGAACAAGGCATGCAAACCTTCGACCAGGCC
TTGTACGGTTTATACAAAACCGGAAAGATCACTTACGAGGACGCCCTGCACGCCGCGGACTCGAAAAACGAGGTCAGGCT
GATGATCAAGTTGGGCGAGGAAGGCAGTATCGAGAAATACGCGGGCCGCAACGACACCCTGTCCGTGGTGGAAGACGATT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A286P3P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.933

100

0.673
  pilU Acinetobacter baylyi ADP1

63.866

95.71

0.611
  pilU Vibrio cholerae strain A1552

56.571

93.834

0.531
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.241

93.298

0.394
  pilT Legionella pneumophila strain Lp02

39.359

91.957

0.362
  pilT Legionella pneumophila strain ERS1305867

39.359

91.957

0.362

Multiple sequence alignment