Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PALA35_RS25570 Genome accession   NZ_CP110346
Coordinates   5496598..5497062 (-) Length   154 a.a.
NCBI ID   WP_124089607.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5491598..5502062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA35_RS25555 (PALA35_05057) nadC 5494119..5494967 (+) 849 WP_269974489.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PALA35_RS25565 (PALA35_05059) - 5495147..5496532 (-) 1386 WP_020751089.1 O-antigen ligase family protein -
  PALA35_RS25570 (PALA35_05060) pilA 5496598..5497062 (-) 465 WP_124089607.1 pilin Machinery gene
  PALA35_RS25575 (PALA35_05061) pilB 5497293..5498993 (+) 1701 WP_269974490.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PALA35_RS25580 (PALA35_05062) pilC 5498997..5500214 (+) 1218 WP_269974491.1 type II secretion system F family protein Machinery gene
  PALA35_RS25585 (PALA35_05063) pilD 5500215..5501087 (+) 873 WP_269974492.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PALA35_RS25590 (PALA35_05064) coaE 5501084..5501695 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  PALA35_RS25595 (PALA35_05065) yacG 5501692..5501892 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16253.62 Da        Isoelectric Point: 9.0066

>NTDB_id=683894 PALA35_RS25570 WP_124089607.1 5496598..5497062(-) (pilA) [Pseudomonas aeruginosa strain PALA35]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSTTPNNTQYDIGFTE
STLLDGSGKSQIKVTDNKDGTVEMVATLGKSSGSAIKGAVITVSRDAQGVWNCKISKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=683894 PALA35_RS25570 WP_124089607.1 5496598..5497062(-) (pilA) [Pseudomonas aeruginosa strain PALA35]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCACGACTCCTAACAATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCAAGGTAACGGACAATAAAGATGGCACCGTTGAGATGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAACTGCA
AAATCTCCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.079

98.701

0.435
  pilA Pseudomonas aeruginosa PAK

42.308

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.83

99.351

0.416
  pilA Vibrio parahaemolyticus RIMD 2210633

38.961

100

0.39
  pilA Vibrio cholerae strain A1552

36.364

100

0.364
  pilA Vibrio cholerae C6706

36.364

100

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.364