Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M0C34_RS17070 Genome accession   NZ_CP096670
Coordinates   3758039..3759742 (+) Length   567 a.a.
NCBI ID   WP_371923088.1    Uniprot ID   -
Organism   Agarivorans sp. TSD2052     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3753039..3764742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0C34_RS17045 (M0C34_17045) pdhR 3753457..3754221 (-) 765 WP_248712872.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  M0C34_RS17050 (M0C34_17050) ampE 3754479..3755333 (-) 855 WP_248712873.1 regulatory signaling modulator protein AmpE -
  M0C34_RS17055 (M0C34_17055) ampD 3755408..3755947 (-) 540 WP_248712874.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  M0C34_RS17060 (M0C34_17060) nadC 3756411..3757250 (+) 840 WP_248712875.1 carboxylating nicotinate-nucleotide diphosphorylase -
  M0C34_RS17065 (M0C34_17065) - 3757505..3758023 (+) 519 WP_248712876.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  M0C34_RS17070 (M0C34_17070) pilB 3758039..3759742 (+) 1704 WP_371923088.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0C34_RS17075 (M0C34_17075) pilC 3759827..3761074 (+) 1248 WP_305883136.1 type II secretion system F family protein Machinery gene
  M0C34_RS17080 (M0C34_17080) pilD 3761077..3761967 (+) 891 WP_248712877.1 A24 family peptidase Machinery gene
  M0C34_RS17085 (M0C34_17085) coaE 3762080..3762682 (+) 603 WP_248712878.1 dephospho-CoA kinase -
  M0C34_RS17090 (M0C34_17090) zapD 3762743..3763483 (+) 741 WP_248712879.1 cell division protein ZapD -
  M0C34_RS17095 (M0C34_17095) yacG 3763483..3763680 (+) 198 WP_248712880.1 DNA gyrase inhibitor YacG -
  M0C34_RS17100 (M0C34_17100) mutT 3763809..3764207 (-) 399 WP_248712881.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62674.99 Da        Isoelectric Point: 5.1359

>NTDB_id=682227 M0C34_RS17070 WP_371923088.1 3758039..3759742(+) (pilB) [Agarivorans sp. TSD2052]
MKNVSINGLASSLAVSFPEKEHQLLELYKRSVQEQRAFSTVLVENELISSRELAEFCESQFGIPLLDLDAIELSEIPQNF
LNEKLIFKHHVLPVYSQNQTLYVAMADPSNVAALEDFGFNFGLHVEALLVEEAKLFKAIQSLVDDSANLDLGDISDDDIS
GLEVSDEDSRLDEKQDNKQDDAPIVVYINKILMDSIRRGASDLHFEPYEKKYRIRFRIDGILHEIASPPINLANRFSARL
KVMARMDIAERRLPQDGRIKLKTGRNKAIDMRVNSLPTMWGEKIVIRILDSSAANLNIDILGYDDAQKKLYLDALSRPQG
MILVTGPTGSGKTVSLYTGLNILNTTEINISTAEDPVEINLPGVNQVQINNRAGLTFASALRAFLRQDPDVVMVGEIRDL
ETAEISIKAAQTGHLVLSTLHTNSAAETLTRMLNMGVPAFNIASSVSLIIAQRLARRLCEHCKKPKMIEANLLPKVGFTQ
SQVEAGIKLFDPVGCEKCTGGFKGRVGIYEVMPMSSAIAKLIMAEGNSIQISEQAVSEGMYSLRMSALEKARLGVTSLSE
VERVTNV

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=682227 M0C34_RS17070 WP_371923088.1 3758039..3759742(+) (pilB) [Agarivorans sp. TSD2052]
TTGAAAAACGTTAGCATAAACGGCCTAGCCTCTAGCTTGGCCGTTTCTTTTCCAGAAAAAGAACACCAGCTACTAGAACT
ATATAAACGCAGTGTGCAAGAACAGCGCGCTTTTAGCACTGTGCTGGTAGAAAACGAGCTCATTTCTAGCCGAGAATTAG
CTGAATTTTGTGAGAGCCAGTTTGGCATTCCGCTGCTAGACTTGGATGCCATCGAATTATCTGAGATCCCGCAAAACTTT
CTCAATGAAAAATTAATCTTTAAACATCACGTATTACCGGTCTATAGTCAAAATCAAACCCTTTATGTGGCTATGGCAGA
TCCTAGCAACGTTGCCGCGCTAGAAGATTTTGGTTTTAATTTTGGGCTACATGTTGAAGCCTTATTGGTTGAAGAAGCCA
AGCTATTTAAGGCGATTCAGTCTTTAGTTGATGATAGCGCTAACCTTGATTTAGGCGACATCTCTGATGACGACATCAGT
GGCCTAGAAGTTAGTGATGAAGACTCCCGGCTAGACGAGAAACAAGACAACAAACAAGATGATGCACCGATTGTTGTTTA
CATTAACAAGATATTAATGGACTCGATTCGCCGCGGCGCCTCTGATTTACACTTTGAACCCTACGAAAAGAAATACCGGA
TTCGCTTTCGTATCGATGGCATTTTGCATGAAATAGCCAGCCCACCAATCAACTTGGCCAACCGCTTCTCAGCGCGCTTA
AAAGTAATGGCGCGCATGGACATTGCCGAACGCCGTTTGCCACAAGATGGTCGCATCAAGCTTAAAACGGGCCGTAATAA
AGCCATCGATATGCGGGTTAACTCGCTACCCACCATGTGGGGCGAAAAAATCGTGATTCGTATTCTCGACTCCTCAGCGG
CTAACTTAAACATAGATATACTCGGTTACGATGACGCGCAGAAAAAGCTCTATTTAGATGCCTTATCCCGCCCACAGGGC
ATGATATTGGTCACCGGCCCGACCGGCTCGGGTAAAACCGTCTCTTTATATACCGGCTTAAACATTCTTAATACTACCGA
GATCAACATCTCAACCGCCGAAGACCCGGTAGAGATTAACCTACCAGGGGTGAACCAAGTACAAATTAATAATCGCGCTG
GTTTAACCTTTGCCAGTGCGCTGCGGGCATTTTTGCGCCAAGATCCCGATGTAGTGATGGTGGGTGAAATTCGTGACTTA
GAAACCGCCGAAATTTCAATTAAAGCGGCGCAAACCGGCCACTTGGTATTATCCACCCTGCATACTAACTCGGCTGCTGA
AACCCTCACCCGCATGTTAAATATGGGCGTACCAGCCTTTAATATCGCCTCCTCAGTCAGTTTAATTATCGCTCAGCGCC
TAGCACGCCGCCTGTGTGAGCATTGCAAAAAACCCAAGATGATAGAGGCCAACTTACTGCCTAAAGTGGGTTTTACTCAA
AGCCAAGTAGAAGCCGGCATAAAATTATTTGACCCGGTGGGCTGTGAAAAATGCACCGGCGGTTTTAAGGGCCGAGTAGG
TATTTATGAAGTGATGCCCATGAGCTCGGCCATTGCCAAGCTCATTATGGCCGAGGGCAACTCGATTCAAATTTCTGAAC
AAGCGGTTAGTGAGGGCATGTATTCACTGCGCATGTCCGCCTTAGAAAAGGCCCGTTTAGGCGTAACTAGCTTATCAGAG
GTGGAGCGAGTCACAAATGTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.25

95.944

0.54
  pilB Acinetobacter baylyi ADP1

54.745

96.649

0.529
  pilB Legionella pneumophila strain ERS1305867

54.579

96.296

0.526
  pilB Vibrio cholerae strain A1552

54.924

93.122

0.511
  pilB Vibrio campbellii strain DS40M4

52.364

97.002

0.508
  pilB Vibrio parahaemolyticus RIMD 2210633

53.992

92.769

0.501
  pilF Neisseria gonorrhoeae MS11

46.702

98.942

0.462
  pilB/pilB1 Synechocystis sp. PCC 6803

39.813

94.356

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.674

95.767

0.37