Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MZ182_RS18150 Genome accession   NZ_CP096169
Coordinates   4082026..4083735 (+) Length   569 a.a.
NCBI ID   WP_248067532.1    Uniprot ID   -
Organism   Shewanella sp. JNE2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4077026..4088735
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZ182_RS18130 (MZ182_18130) ampE 4077721..4078572 (-) 852 WP_248067541.1 beta-lactamase regulator AmpE -
  MZ182_RS18135 (MZ182_18135) ampD 4078779..4079321 (-) 543 WP_248067538.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MZ182_RS18140 (MZ182_18140) nadC 4079651..4080538 (+) 888 WP_011787905.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MZ182_RS18145 (MZ182_18145) - 4081466..4081942 (+) 477 WP_248067534.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  MZ182_RS18150 (MZ182_18150) pilB 4082026..4083735 (+) 1710 WP_248067532.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MZ182_RS18155 (MZ182_18155) pilC 4084169..4085434 (+) 1266 WP_011787902.1 type II secretion system F family protein Machinery gene
  MZ182_RS18160 (MZ182_18160) pilD 4085614..4086552 (+) 939 WP_248067529.1 prepilin peptidase Machinery gene
  MZ182_RS18165 (MZ182_18165) coaE 4086896..4087513 (+) 618 WP_248067527.1 dephospho-CoA kinase -
  MZ182_RS18170 (MZ182_18170) zapD 4087506..4088240 (+) 735 WP_011787899.1 cell division protein ZapD -
  MZ182_RS18175 (MZ182_18175) yacG 4088398..4088607 (+) 210 WP_011920039.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62633.10 Da        Isoelectric Point: 6.3063

>NTDB_id=680199 MZ182_RS18150 WP_248067532.1 4082026..4083735(+) (pilB) [Shewanella sp. JNE2]
MSTTGLHLGLSTLFIRKGLLNEEQMAGAIAKSRHNKQGLVATLVQGQLVSSRTIAELCYEEYGTPLLDLAEFDIRAIPED
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDITSLDLGGMDEAA
LAGIEITDTDRRNEEPTGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
SPAQITQGFTTFKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVLQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=680199 MZ182_RS18150 WP_248067532.1 4082026..4083735(+) (pilB) [Shewanella sp. JNE2]
ATGTCTACAACAGGCCTTCATTTAGGTTTATCCACTCTCTTTATCCGTAAGGGTCTGCTAAATGAAGAGCAGATGGCAGG
GGCTATTGCAAAATCCCGTCATAATAAACAAGGGTTAGTGGCGACCTTAGTACAAGGCCAGTTAGTCTCATCCCGCACCA
TTGCCGAGCTTTGCTATGAAGAATATGGCACACCGCTACTGGATTTAGCGGAATTTGATATACGAGCCATTCCCGAAGAT
TTTTTAAATAAAAAACTGATTGAAAAACACCGCTGTTTACCGCTATTTAAGCGCGGAAATCGCCTCTATATCGCCACCTC
CGATCCCACCAATATTGCCGCCCTCGAAGATTTTCAATTTAGTGCCGGCCTGCATGCCGAAGCGATTTTAGTTGAAGAAG
ATAAGCTCGCTAAAGCCCTTGAAAAAATCCTTGAAGAAGATATTACCAGCCTCGATTTAGGTGGCATGGATGAAGCGGCG
CTGGCGGGGATAGAAATTACCGATACCGATAGACGCAATGAAGAACCGACAGGTGACAGCAGTGACGATGCGCCCATTGT
TATTTATATCAATAAAATTTTAACCGATGCGATTCGTAAGGGCGCCTCCGACTTACACTTTGAACCCTACGAAAAACGCT
ATCGCATTCGTTTTCGGATTGACGGTATTTTGCATGAGGTATCAGAGCCGCCCATTAGCCTTGCGGGCCGTTTATCCGCC
CGTTTAAAGGTGATGTCAAAACTGGATATTGCCGAGCGGCGCGTACCACAGGATGGCCGAATTAAAATGAAGCTCTCCCG
CACTAAGTCTATCGATTTTCGGGTTAGCACCTTGCCTACCCTGTGGGGCGAAAAAATTGTCATGCGGATCTTGGACTCCT
CCTCCGCACAACTAGGTATCGAAAAACTCGGCTACGAGCCCGATCAAGAAAAGTTATACCTTGAAATGCTCGCTAAACCC
CAAGGGATGATTTTAGTCACAGGACCAACGGGCTCGGGTAAAACCGTTTCCCTCTACACGGGGTTAAATATCCTCAATAC
CGCCGAGCGCAATATTTCGACCGCTGAAGATCCGGTAGAGATTAACCTTGAGGGGGTCAACCAAGTTCATATTAACCTAA
AAGCTGGGCTGACATTTGCCTCAGCACTACGTTCTTTTTTGCGCCAAGACCCCGACGTGGTGATGGTGGGTGAGATACGC
GATCTTGAAACCGCCGAAATTGCCATTAAAGCAGCACAAACGGGACATTTAGTGTTGTCGACCCTACACACTAACTCGGC
CGCCGAAACCCTAACCCGTTTAATCAATATGGGCGTACCTGGATACAATATCGCCAGCTCGGTTAACCTTATTATTGCCC
AGCGCCTTGCCCGCAGACTTTGCCCAGAATGCAAACAAGCAGAGGTGATCCCCGAGCATGAACTCTTACGTTTAGGTTTT
TCTCCTGCACAAATTACCCAAGGGTTTACTACCTTTAAACCAGTGGGGTGTGAACATTGCTCTGGTGGTTATAAGGGGCG
AGTAGGGATTTATGAAGTGATGAAAATGTCCGATGAAATTGCCCGTACCATTATGGAAGGCGGCAACTCGTTGCAAATTG
CTTCCCAAGCGAAAGCACAAGGTATGCGCGATTTACGTCAATCGGGTTTGTTAAAAGTATTACAGGGCGTCACCAGTATT
GCCGAAGTGAATCGGGTGACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.893

98.594

0.591
  pilB Acinetobacter baumannii D1279779

59.633

95.782

0.571
  pilB Legionella pneumophila strain ERS1305867

55.654

99.473

0.554
  pilB Vibrio parahaemolyticus RIMD 2210633

51.579

100

0.517
  pilB Vibrio cholerae strain A1552

56.371

91.037

0.513
  pilB Vibrio campbellii strain DS40M4

51.06

99.473

0.508
  pilF Neisseria gonorrhoeae MS11

48.41

99.473

0.482
  pilF Thermus thermophilus HB27

39.576

99.473

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

98.243

0.38