Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MZ018_RS03000 Genome accession   NZ_CP096163
Coordinates   705063..706772 (+) Length   569 a.a.
NCBI ID   WP_011920035.1    Uniprot ID   -
Organism   Shewanella sp. JNE10-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 700063..711772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZ018_RS02980 (MZ018_02980) ampE 700773..701624 (-) 852 WP_248084755.1 beta-lactamase regulator AmpE -
  MZ018_RS02985 (MZ018_02985) ampD 701824..702366 (-) 543 WP_115016962.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MZ018_RS02990 (MZ018_02990) nadC 702712..703599 (+) 888 WP_248084754.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MZ018_RS02995 (MZ018_02995) - 704524..704973 (+) 450 WP_248084753.1 pilin -
  MZ018_RS03000 (MZ018_03000) pilB 705063..706772 (+) 1710 WP_011920035.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MZ018_RS03005 (MZ018_03005) pilC 706944..708209 (+) 1266 WP_011787902.1 type II secretion system F family protein Machinery gene
  MZ018_RS03010 (MZ018_03010) pilD 708409..709335 (+) 927 WP_248084752.1 prepilin peptidase Machinery gene
  MZ018_RS03015 (MZ018_03015) coaE 709679..710296 (+) 618 WP_248067527.1 dephospho-CoA kinase -
  MZ018_RS03020 (MZ018_03020) zapD 710289..711023 (+) 735 WP_248084751.1 cell division protein ZapD -
  MZ018_RS03025 (MZ018_03025) yacG 711181..711390 (+) 210 WP_011920039.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62643.18 Da        Isoelectric Point: 6.4447

>NTDB_id=680139 MZ018_RS03000 WP_011920035.1 705063..706772(+) (pilB) [Shewanella sp. JNE10-2]
MPTTGLHLGLSTLFIRKGLLNEEQMAGAIAKSRHNKQGLVATLVQGKLVSSRTIAELCYEEYGTPLLDLAEFDIRAIPED
FLNKKLIEKHRCLPLFKRGNRLYIATSDPTNIAALEDFQFSAGLHAEAILVEEDKLAKALEKILEEDITSLDLGGMDEAA
LAGIEITDTDRRNEEPTGDSSDDAPIVIYINKILTDAIRKGASDLHFEPYEKRYRIRFRIDGILHEVSEPPISLAGRLSA
RLKVMSKLDIAERRVPQDGRIKMKLSRTKSIDFRVSTLPTLWGEKIVMRILDSSSAQLGIEKLGYEPDQEKLYLEMLAKP
QGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGVNQVHINLKAGLTFASALRSFLRQDPDVVMVGEIR
DLETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLINMGVPGYNIASSVNLIIAQRLARRLCPECKQAEVIPEHELLRLGF
SPAQITQGFTTFKPVGCEHCSGGYKGRVGIYEVMKMSDEIARTIMEGGNSLQIASQAKAQGMRDLRQSGLLKVLQGVTSI
AEVNRVTSF

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=680139 MZ018_RS03000 WP_011920035.1 705063..706772(+) (pilB) [Shewanella sp. JNE10-2]
ATGCCAACTACAGGCCTTCATCTTGGTTTATCCACTCTCTTTATCCGTAAGGGTCTGCTAAATGAAGAGCAGATGGCAGG
GGCTATTGCAAAATCCCGTCATAATAAACAAGGGTTAGTGGCGACCTTAGTACAAGGCAAGTTAGTCTCATCCCGCACCA
TTGCCGAGCTTTGCTATGAAGAATATGGCACACCCTTACTGGATTTAGCGGAATTTGATATACGAGCCATTCCCGAAGAT
TTTTTAAATAAAAAACTGATTGAAAAACACCGCTGTTTACCGCTATTTAAGCGCGGAAATCGCCTCTATATCGCCACCTC
CGATCCCACCAATATTGCCGCCCTTGAAGATTTTCAATTTAGCGCAGGCCTGCATGCCGAAGCGATTTTGGTTGAAGAAG
ATAAGCTCGCTAAAGCCCTTGAAAAAATCCTTGAAGAAGATATTACCAGCCTCGATTTAGGTGGCATGGATGAAGCGGCG
CTGGCGGGGATAGAAATTACCGATACCGATAGACGCAATGAAGAACCGACAGGTGACAGCAGTGACGATGCGCCCATTGT
TATTTATATCAATAAAATTTTAACCGATGCGATTCGTAAGGGTGCCTCCGACTTACACTTTGAACCCTACGAAAAACGCT
ATCGCATCCGTTTTCGGATTGACGGTATTTTGCATGAGGTATCAGAGCCGCCCATTAGCCTTGCGGGCCGTTTATCCGCC
CGTTTAAAGGTGATGTCAAAACTGGATATTGCCGAGCGGCGCGTACCACAGGATGGCCGAATTAAAATGAAGCTCTCCCG
CACTAAGTCTATCGATTTTCGGGTTAGCACCTTGCCTACCCTGTGGGGTGAAAAAATTGTAATGCGGATCTTGGACTCCT
CCTCCGCACAACTAGGTATCGAAAAACTCGGCTACGAGCCCGATCAAGAAAAGTTATACCTTGAAATGCTGGCTAAACCC
CAAGGGATGATTTTAGTCACAGGACCAACGGGCTCGGGTAAAACCGTTTCCCTCTACACGGGGTTAAATATCCTCAATAC
CGCCGAGCGCAATATTTCGACCGCTGAAGATCCGGTAGAAATTAACCTTGAGGGGGTCAACCAAGTTCATATTAACCTAA
AAGCCGGGCTGACATTTGCCTCAGCACTACGTTCTTTTTTACGCCAAGATCCCGACGTGGTGATGGTGGGTGAGATACGC
GATCTTGAAACCGCCGAAATTGCCATTAAAGCCGCACAAACGGGACATTTAGTGTTGTCGACCCTACACACTAACTCGGC
CGCCGAAACCTTAACCCGTTTAATCAATATGGGTGTACCTGGATACAATATCGCCAGCTCGGTTAACCTTATTATTGCCC
AGCGCCTTGCCCGCAGACTTTGCCCAGAATGCAAACAAGCAGAGGTGATCCCCGAGCATGAACTCTTACGTTTAGGTTTT
TCTCCTGCACAAATTACCCAAGGGTTTACTACCTTTAAACCAGTGGGATGTGAACATTGCTCTGGTGGTTATAAGGGGCG
AGTGGGGATTTATGAAGTGATGAAAATGTCCGATGAAATTGCCCGTACCATTATGGAAGGCGGCAACTCGTTGCAAATTG
CTTCCCAAGCGAAAGCACAAGGTATGCGCGATTTACGTCAATCGGGTTTATTAAAAGTATTACAGGGCGTCACCAGTATT
GCCGAAGTGAATCGGGTAACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.893

98.594

0.591
  pilB Acinetobacter baumannii D1279779

59.633

95.782

0.571
  pilB Legionella pneumophila strain ERS1305867

55.83

99.473

0.555
  pilB Vibrio cholerae strain A1552

56.371

91.037

0.513
  pilB Vibrio parahaemolyticus RIMD 2210633

51.228

100

0.513
  pilB Vibrio campbellii strain DS40M4

51.06

99.473

0.508
  pilF Neisseria gonorrhoeae MS11

48.41

99.473

0.482
  pilF Thermus thermophilus HB27

39.576

99.473

0.394
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.64

98.243

0.38