Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   MZ018_RS03010 Genome accession   NZ_CP096163
Coordinates   708409..709335 (+) Length   308 a.a.
NCBI ID   WP_248084752.1    Uniprot ID   -
Organism   Shewanella sp. JNE10-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 703409..714335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MZ018_RS02995 (MZ018_02995) - 704524..704973 (+) 450 WP_248084753.1 pilin -
  MZ018_RS03000 (MZ018_03000) pilB 705063..706772 (+) 1710 WP_011920035.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MZ018_RS03005 (MZ018_03005) pilC 706944..708209 (+) 1266 WP_011787902.1 type II secretion system F family protein Machinery gene
  MZ018_RS03010 (MZ018_03010) pilD 708409..709335 (+) 927 WP_248084752.1 prepilin peptidase Machinery gene
  MZ018_RS03015 (MZ018_03015) coaE 709679..710296 (+) 618 WP_248067527.1 dephospho-CoA kinase -
  MZ018_RS03020 (MZ018_03020) zapD 710289..711023 (+) 735 WP_248084751.1 cell division protein ZapD -
  MZ018_RS03025 (MZ018_03025) yacG 711181..711390 (+) 210 WP_011920039.1 DNA gyrase inhibitor YacG -
  MZ018_RS03030 (MZ018_03030) mutT 711442..711846 (+) 405 WP_011787897.1 8-oxo-dGTP diphosphatase MutT -
  MZ018_RS03035 (MZ018_03035) - 712292..712828 (+) 537 WP_248067524.1 thioredoxin family protein -
  MZ018_RS03040 (MZ018_03040) - 713092..714018 (-) 927 WP_248067521.1 exopolyphosphatase -

Sequence

Protein


Download         Length: 308 a.a.        Molecular weight: 34364.69 Da        Isoelectric Point: 6.5907

>NTDB_id=680141 MZ018_RS03010 WP_248084752.1 708409..709335(+) (pilD) [Shewanella sp. JNE10-2]
MTEFITLLGQSFEQSPWLFVTLSFIFAATIGSFLNVVIHRLPIMMKREWQQECNQYLQEYHADIVNQVGIDRLNKPIDSY
PEKYNIVAPGSSCPQCKTAIKPWHNLPVLGWLMLRGKCAACHAPISTRYPIIELITGLLIAALAWHFGPCWQFVFASLLT
FVLIALTGIDLDEMLLPDQLTLPLLWLGLLLNLHHTFASPTDAIIGAAAGYLSLWSIFWVFKLLTGKEGMGYGDFKLLAV
FGAWLGWQMLPLVILLSSLVGAVVGIGMIISKRNQMDNPIPFGPYIAAAGWIALVWGNTIVDWYLGTL

Nucleotide


Download         Length: 927 bp        

>NTDB_id=680141 MZ018_RS03010 WP_248084752.1 708409..709335(+) (pilD) [Shewanella sp. JNE10-2]
ATGACTGAATTTATCACTTTATTAGGCCAATCTTTTGAACAGAGTCCTTGGCTCTTCGTCACCTTAAGCTTTATTTTTGC
CGCGACTATTGGCAGTTTTCTGAATGTGGTCATCCACCGACTCCCTATCATGATGAAGCGTGAATGGCAGCAGGAATGTA
ATCAATATTTACAGGAATACCATGCAGATATCGTTAACCAAGTCGGTATCGATAGGCTTAATAAACCCATAGATAGCTAT
CCCGAAAAGTACAATATTGTGGCGCCAGGCTCATCTTGCCCTCAGTGTAAAACCGCCATCAAACCTTGGCATAATTTGCC
AGTGCTCGGTTGGTTAATGCTTAGGGGGAAATGCGCCGCCTGTCATGCACCTATCTCGACCCGTTATCCGATTATTGAAT
TAATCACAGGTTTACTGATAGCAGCTCTAGCTTGGCATTTTGGCCCCTGTTGGCAATTTGTTTTTGCCTCTTTACTCACC
TTTGTATTGATTGCGCTGACAGGTATAGATCTGGATGAAATGTTGTTACCGGATCAGTTAACACTGCCCCTACTGTGGCT
TGGGCTATTACTCAATTTACACCATACCTTTGCTAGCCCAACGGATGCCATTATCGGTGCAGCCGCAGGCTATTTAAGCC
TATGGTCAATTTTCTGGGTGTTTAAGTTACTCACAGGCAAAGAAGGTATGGGCTATGGCGACTTTAAACTGTTGGCCGTT
TTTGGTGCTTGGCTTGGCTGGCAAATGTTGCCACTGGTGATTTTACTCTCCTCATTAGTGGGTGCTGTTGTGGGCATTGG
CATGATTATCAGCAAGCGTAATCAGATGGATAACCCTATTCCCTTTGGCCCTTATATTGCCGCCGCAGGCTGGATTGCAC
TGGTTTGGGGAAACACTATCGTCGACTGGTATTTAGGGACACTATAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(25-147)

Type IV leader peptidase family (Peptidase_A24)

(159-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.824

97.727

0.516
  pilD Vibrio campbellii strain DS40M4

51

97.403

0.497
  pilD Acinetobacter baumannii D1279779

49.135

93.831

0.461
  pilD Acinetobacter nosocomialis M2

49.135

93.831

0.461
  pilD Neisseria gonorrhoeae MS11

43.448

94.156

0.409