Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OBG92_RS23250 Genome accession   NZ_CP109657
Coordinates   4914441..4915850 (-) Length   469 a.a.
NCBI ID   WP_034079109.1    Uniprot ID   A0A2R3IVK2
Organism   Pseudomonas aeruginosa strain Zw26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4909441..4920850
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OBG92_RS23240 (OBG92_04574) - 4911606..4912784 (-) 1179 WP_058145729.1 substrate-binding domain-containing protein -
  OBG92_RS23245 (OBG92_04575) pilC 4913227..4914441 (-) 1215 WP_033996852.1 type II secretion system inner membrane protein GspF Machinery gene
  OBG92_RS23250 (OBG92_04576) pilB 4914441..4915850 (-) 1410 WP_034079109.1 type II secretion system ATPase GspE Machinery gene
  OBG92_RS23255 (OBG92_04577) gspD 4915847..4918258 (-) 2412 WP_058145730.1 type II secretion system secretin GspD -
  OBG92_RS23260 (OBG92_04578) - 4918255..4918821 (-) 567 WP_034079105.1 type II secretion system protein M -
  OBG92_RS23265 (OBG92_04579) gspL 4918814..4920013 (-) 1200 WP_034079103.1 type II secretion system protein GspL -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 50875.31 Da        Isoelectric Point: 5.7931

>NTDB_id=679703 OBG92_RS23250 WP_034079109.1 4914441..4915850(-) (pilB) [Pseudomonas aeruginosa strain Zw26]
MSLLPYAWAKAQRALLRPGADGATLLVSPRTPGWAISEVRQRHAVASLESVRDDELDTLLASAYSDTGSAAAVVGAAESE
VDLDRLLDDIPEVTDLLDTQDGAPVIRMINALLTQAARDEASDIHIEPFETHSVVRYRVDGALRDVVAPRKALHAALVSR
IKIMAQLDIAEKRLPQDGRIALRVAGRPIDIRVSTVPTGHGERVVMRLLDKQAGRLKLETLGMAPGVLAPLDQLIRQPHG
IVLVTGPTGSGKTTTLYAALARLDASTSNILTVEDPVEYDLPGISQIQVNARIDMTFAVALRAILRQDPDIIMIGEIRDL
ETAQIAVQASLTGHLVLATLHTNDAVSAVTRLVDMGVEPFLLASSMLGVLAQRLVRRLCTHCRLEEDGGWRAVGCPACNH
TGYSGRTGIHELFVIDDEIRRLVHQGRAEQDLREAARAAGMRSMREDGERWIASGATTLEEILRVTRDA

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=679703 OBG92_RS23250 WP_034079109.1 4914441..4915850(-) (pilB) [Pseudomonas aeruginosa strain Zw26]
GTGAGCCTGCTGCCCTACGCCTGGGCCAAGGCGCAGCGCGCCCTGCTGCGGCCGGGCGCCGACGGCGCGACCCTGCTGGT
CAGTCCGCGCACGCCCGGCTGGGCGATCAGCGAGGTGCGCCAGCGGCATGCCGTGGCCAGTCTCGAGAGCGTCCGCGACG
ACGAACTCGACACCCTGCTGGCCAGCGCCTATTCCGACACCGGCAGCGCCGCGGCGGTGGTCGGCGCAGCGGAGAGCGAG
GTCGACCTCGACCGCCTGCTGGACGACATCCCCGAGGTCACCGACCTGCTCGACACCCAGGACGGCGCGCCGGTGATCCG
CATGATCAACGCGCTGCTGACCCAGGCCGCGCGCGACGAGGCCAGCGACATCCACATCGAGCCCTTCGAGACCCATTCGG
TGGTGCGCTACCGCGTCGACGGCGCCCTGCGCGACGTGGTCGCGCCGCGCAAGGCGCTGCACGCGGCGCTGGTATCGCGG
ATCAAGATCATGGCGCAGCTGGACATCGCCGAGAAACGCCTGCCCCAGGACGGCCGCATCGCCCTGCGGGTGGCCGGGCG
ACCGATCGACATCCGCGTCTCCACCGTACCCACCGGGCACGGCGAGCGGGTGGTGATGCGTCTGCTGGACAAGCAGGCCG
GGCGCCTGAAGCTGGAGACCCTGGGCATGGCCCCGGGGGTACTGGCGCCGCTGGACCAGCTGATCCGCCAGCCGCACGGC
ATCGTGCTGGTCACCGGGCCCACCGGCAGCGGCAAGACCACCACCCTCTACGCCGCCCTGGCGCGGCTCGACGCCAGCAC
CAGCAACATCCTCACCGTCGAGGACCCGGTGGAGTACGACCTGCCCGGGATCAGCCAGATCCAGGTCAACGCGCGCATCG
ACATGACCTTCGCCGTGGCCCTGCGGGCGATCCTCCGGCAGGACCCGGACATCATCATGATCGGCGAGATCCGCGACCTG
GAGACCGCGCAGATCGCCGTGCAGGCCTCGCTCACCGGGCACCTGGTGCTGGCCACGCTGCACACCAACGACGCGGTGTC
GGCGGTGACCCGGCTGGTGGACATGGGCGTCGAGCCATTCCTGCTCGCCTCGTCGATGCTCGGCGTGCTCGCCCAGCGCC
TGGTGCGGCGCCTGTGCACGCATTGCCGGCTCGAAGAGGACGGCGGTTGGCGCGCGGTCGGCTGCCCGGCCTGCAACCAC
ACCGGCTACAGCGGGCGCACCGGCATCCACGAGCTGTTCGTGATCGACGACGAGATCCGCCGCCTGGTCCACCAGGGCCG
CGCCGAGCAGGATCTCCGCGAAGCCGCCCGCGCCGCCGGCATGCGCAGCATGCGCGAGGACGGCGAGCGCTGGATCGCCA
GCGGCGCCACCACCCTGGAGGAAATCCTCCGCGTGACCCGGGACGCCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(111-377)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2R3IVK2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

45.519

90.405

0.412
  pilB/pilB1 Synechocystis sp. PCC 6803

41.113

99.574

0.409
  pilF Thermus thermophilus HB27

50.663

80.384

0.407
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.462

83.156

0.403
  pilF Neisseria gonorrhoeae MS11

47.208

84.009

0.397
  pilB Acinetobacter baumannii D1279779

41.629

94.243

0.392
  pilB Vibrio campbellii strain DS40M4

46.329

84.222

0.39
  pilB Vibrio parahaemolyticus RIMD 2210633

46.329

84.222

0.39
  pilB Legionella pneumophila strain ERS1305867

45.501

82.942

0.377
  pilB Acinetobacter baylyi ADP1

45.361

82.729

0.375