Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M0D45_RS05635 Genome accession   NZ_CP096139
Coordinates   1367380..1369116 (-) Length   578 a.a.
NCBI ID   WP_260817739.1    Uniprot ID   -
Organism   Xanthomonas prunicola strain MAI5069     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1362380..1374116
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0D45_RS05620 (M0D45_05610) - 1363557..1365170 (+) 1614 WP_260817738.1 HAMP domain-containing sensor histidine kinase -
  M0D45_RS05625 (M0D45_05615) pilR 1365441..1366895 (+) 1455 WP_313905392.1 sigma-54 dependent transcriptional regulator Regulator
  M0D45_RS05630 (M0D45_05620) - 1367193..1367312 (-) 120 WP_101362453.1 pilus assembly protein -
  M0D45_RS05635 (M0D45_05625) pilB 1367380..1369116 (-) 1737 WP_260817739.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0D45_RS05640 (M0D45_05630) - 1369209..1369817 (-) 609 WP_260817740.1 methionine biosynthesis protein MetW -
  M0D45_RS05645 (M0D45_05635) - 1369810..1370844 (-) 1035 WP_260818124.1 glycosyltransferase -
  M0D45_RS05650 (M0D45_05640) - 1370847..1371644 (-) 798 WP_260817741.1 glycosyltransferase family A protein -
  M0D45_RS05655 (M0D45_05645) - 1371646..1372026 (-) 381 WP_260817742.1 SMR family transporter -
  M0D45_RS05660 (M0D45_05650) comP 1372349..1372762 (+) 414 WP_260817743.1 pilin Machinery gene

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62676.75 Da        Isoelectric Point: 5.6006

>NTDB_id=679617 M0D45_RS05635 WP_260817739.1 1367380..1369116(-) (pilB) [Xanthomonas prunicola strain MAI5069]
MNTAVTTNLVGITGIARRLVQDGAIDESAARGAMDQAAAAKIPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSAFDAN
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNASFGSSLG
NDDDEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTPAQIAAGIELYEAVGCEECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQSIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=679617 M0D45_RS05635 WP_260817739.1 1367380..1369116(-) (pilB) [Xanthomonas prunicola strain MAI5069]
ATGAACACCGCTGTTACGACGAATCTAGTCGGTATCACGGGAATCGCTCGTCGCCTGGTGCAGGACGGTGCGATTGACGA
ATCCGCTGCTCGCGGCGCCATGGACCAGGCTGCGGCCGCCAAGATCCCGCTTCCTCAGTGGTTTGCCGAAAAAAAACTGG
TATCGGCCGCGCAGCTTGCTGCCGCAAACGCCGTTGAGTTCGGCATGCCGCTGATGGATGTGTCGGCGTTCGACGCCAAT
CAGAACGCGGTCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
ATTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGATATCAAGTTCCATACGAACCTCGTGGTCGAGCCTA
TCCTGGTCGACGAGGACCAGATTCGTCGCACCTTGGAGCAGTGGCAGGCAAGTAACGCTTCGTTTGGTTCGTCGTTGGGG
AACGACGACGACGAGATGGGGGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAAGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGTGGAGCCTCCGACA
TCCATTTCGAGCCCTATGAAGACGATTACCGGGTGCGCTTGCGGATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCG
GTCAAGCTGAGCCAGCGCATCGCCGCCCGCCTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCAGCCAAGCTGGGCATCGAGAAGCTGGGTTACGAAGCGGACCAGCAGAAG
CTGTTCCTGGATGCGATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCCGGCAAGACGGTGTCGCT
GTATACGGCCTTGGGCATCCTCAACGACGAGACGCGCAACATCTCCACCGCCGAGGATCCGGTGGAAATCCGCCTGCCCG
GCGTCAATCAGGTCCAGCAGAACAACAAGCGCGGCATGACGTTTGCCGCGGCCTTGCGCTCGTTCCTGCGCCAGGACCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACTGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GCTATCGACGCTGCACACCAACGATGCACCGCAGACCATTGCGCGCTTGATGAACATGGGCATTGCGCCATACAACATCA
CTTCGTCGGTGACCCTGGTGATCGCGCAGCGTCTGGCGCGTCGGTTGTGCAACAACTGCAAGCGTAAGTCGACCTTGCCC
GAGCATGCGTTGCTGGCCGAAGGCTTCACGCCGGCGCAGATCGCTGCCGGTATCGAACTGTATGAGGCGGTCGGTTGCGA
GGAGTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTATCAGGTGATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAAAGTATCGGCATCCGTGATCTGCGCCAGTCGGCCTTGATG
AAGGCCGCGCATGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-152)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.458

98.27

0.545
  pilB Acinetobacter baylyi ADP1

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.513

99.827

0.524
  pilB Vibrio cholerae strain A1552

48.958

99.654

0.488
  pilF Neisseria gonorrhoeae MS11

48.239

98.27

0.474
  pilB Vibrio campbellii strain DS40M4

46.046

98.443

0.453
  pilB Vibrio parahaemolyticus RIMD 2210633

46.25

96.886

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

35.796

100

0.377