Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   LAB08_RS01865 Genome accession   NZ_AP017423
Coordinates   407992..410073 (+) Length   693 a.a.
NCBI ID   WP_096513793.1    Uniprot ID   -
Organism   Pseudomonas izuensis strain LAB-08     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 402992..415073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LAB08_RS01845 (LAB08_R03690) comM 405207..406268 (+) 1062 WP_096513797.1 pilus assembly protein PilM Machinery gene
  LAB08_RS01850 (LAB08_R03700) - 406268..406834 (+) 567 WP_096513796.1 PilN domain-containing protein -
  LAB08_RS01855 (LAB08_R03710) pilO 406831..407454 (+) 624 WP_096513795.1 type 4a pilus biogenesis protein PilO -
  LAB08_RS01860 (LAB08_R03720) - 407451..407978 (+) 528 WP_096513794.1 pilus assembly protein PilP -
  LAB08_RS01865 (LAB08_R03730) pilQ 407992..410073 (+) 2082 WP_096513793.1 type IV pilus secretin PilQ Machinery gene
  LAB08_RS01870 (LAB08_R03740) aroK 410078..410596 (+) 519 WP_056725695.1 shikimate kinase AroK -
  LAB08_RS01875 (LAB08_R03750) aroB 410744..411844 (+) 1101 WP_096513792.1 3-dehydroquinate synthase -
  LAB08_RS01880 (LAB08_R03760) - 411855..413456 (+) 1602 WP_096513791.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75190.54 Da        Isoelectric Point: 5.5812

>NTDB_id=67908 LAB08_RS01865 WP_096513793.1 407992..410073(+) (pilQ) [Pseudomonas izuensis strain LAB-08]
MNRIFSTLGMSLWIALLSPMVQAANLKALDVAALPGDRVELKLAFDGQPPRPHGYTTESPARIALDLPGVVSQLANKTRD
LGSGNARSATVVEVKDRTRLIINLTQLTPYDTRVEGNNLFVVVGQGAKSAAPRQPAAVVPTPTPAPARAPATTTKAIRAV
DFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIVLGFTKTRLPEPLRVRLDVKDFATPVQFVNASATGDKATITIEPSGAF
DYSTYQTDNKLTVSIRPMTVDDLQKRNADRYAYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNVP
WDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEAKV
DERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVSNG
ANGSSTIGTPGSTSTNTPFVDMGAAANTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=67908 LAB08_RS01865 WP_096513793.1 407992..410073(+) (pilQ) [Pseudomonas izuensis strain LAB-08]
ATGAACAGGATTTTCTCCACCCTCGGTATGTCGCTATGGATAGCGCTGCTTTCGCCGATGGTACAAGCGGCCAACCTCAA
GGCGCTGGATGTCGCTGCGCTGCCGGGGGACCGCGTCGAGTTGAAGCTGGCGTTCGATGGGCAACCTCCGAGGCCCCATG
GGTATACGACAGAGTCCCCTGCACGGATCGCACTGGACTTGCCGGGTGTCGTCAGTCAGCTGGCGAACAAGACTCGCGAC
CTTGGCAGTGGCAATGCCCGAAGCGCGACGGTGGTCGAGGTCAAGGACCGCACGAGGTTGATCATCAATCTGACCCAGTT
GACCCCTTACGACACACGGGTCGAAGGCAATAACCTGTTTGTGGTGGTGGGCCAGGGCGCCAAAAGCGCAGCACCCAGGC
AGCCGGCCGCTGTAGTTCCAACTCCGACCCCGGCCCCGGCGCGTGCACCTGCAACAACGACGAAGGCTATCCGCGCGGTG
GATTTTCAGCGCGGCACTCAGGGTGAAGGCAATGTGGTAATCGATTTGTCGGATCCGTCCATCGCGCCAGACATTCAGGA
GCGCGAGGGCAAGATCGTTCTCGGTTTCACCAAGACCCGATTGCCCGAGCCGCTACGCGTGCGCCTGGACGTCAAGGACT
TCGCCACACCGGTGCAATTCGTCAATGCCAGCGCCACAGGCGACAAGGCCACGATCACGATCGAGCCCAGCGGTGCCTTC
GATTATTCGACCTATCAGACCGACAACAAGCTGACCGTGAGCATTCGCCCGATGACGGTAGATGACCTGCAGAAGCGTAA
CGCCGACCGCTACGCCTACAGCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTACTGCAACTGA
TCGCCGATTTCACCAATCTCAATCTGGTGGCCAGTGACACCGTGCAGGGTGGCATCACGTTGCGTTTGCAAAACGTGCCC
TGGGATCAGGCGCTGGACCTGGTGTTGAAAACCAAGGGGCTGGACAAACGCAAGATCGGCAATGTGCTGTTGGTGGCGCC
GGCCGATGAAATCGCCGCCCGGGAACGCCAGGAGCTGGAGTCGCAGAAACAGATCGCCGAGCTGGCACCGCTGCGCCGGG
AACTGCTGCAGGTCAATTACGCCAAGGCAGCCGATATCGCCAAGTTGTTCCAGTCGGTGACCAGTGCCGAGGCGAAAGTC
GACGAACGGGGTTCGATCACCGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCGACTCGACGAGCT
GCGGCGAATCGTGGCGCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAAGCACGGATCGTCGAAGCCAATGTCGATT
ACGACAAGAGCCTCGGCGTGCGCTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAACACGTCAGGCGTGAGCAACGGC
GCCAACGGGTCGTCCACCATCGGCACGCCGGGAAGCACCAGCACCAATACACCGTTCGTCGATATGGGGGCCGCGGCCAA
TACCTCAGGGATCGGCATCGCGTTCATCACCGATAACGTCTTGCTTGACCTGGAGCTGACGGCCATGGAGAAAACCGGCA
ACGGCGAAATCGTCTCGCAACCCAAGGTGGTCACGTCCGACAAGGAAACCGCAAAAATCCTCAAGGGCACCGAAATTCCC
TATCAGGAAGCCAGCTCCAGCGGCGCTACTTCGGTCTCGTTCAAGGAGGCTTCGCTGTCGCTGGAAGTGACGCCGCAAAT
CACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCGGACTACCTGAACAAGGTGCAGGATGTGC
CGCCAATCAAGAAAAACGAGGTCAATGCAAAGGTGCTGGTGAACGATGGCGAGACCATCGTCATTGGTGGCGTTTTCTCA
AATACTCAAAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGACGT
GGTTTCGGAGAAAAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.75
  comQ Acinetobacter baylyi ADP1

41.404

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.306

100

0.411
  pilQ Acinetobacter baumannii D1279779

38.306

100

0.411
  pilQ Legionella pneumophila strain Lp02

38.824

98.124

0.381
  pilQ Legionella pneumophila strain ERS1305867

38.633

97.114

0.375

Multiple sequence alignment