Detailed information NTG0677928

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MXM90_RS08725
• Genome accession: NZ_CP095737
• Coordinates: 1759719..1760366 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis strain JNU 534
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1754719..1765366

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MXM90_RS08695 (MXM90_08695)	atpF	1754780..1755286 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
MXM90_RS08700 (MXM90_08700)	atpB	1755301..1756014 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
MXM90_RS08705 (MXM90_08705)	-	1756059..1756274 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
MXM90_RS08710 (MXM90_08710)	-	1756458..1757234 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
MXM90_RS08715 (MXM90_08715)	comEC	1757519..1759078 (-)	1560	WP_345893333.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
MXM90_RS08720 (MXM90_08720)	comEC	1759096..1759629 (-)	534	WP_235589824.1	ComEC/Rec2 family competence protein	Machinery gene
MXM90_RS08725 (MXM90_08725)	comEA	1759719..1760366 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
MXM90_RS08730 (MXM90_08730)	-	1760426..1761787 (-)	1362	WP_141347622.1	ABC transporter permease	-
MXM90_RS08735 (MXM90_08735)	-	1761784..1762716 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
MXM90_RS08740 (MXM90_08740)	-	1762821..1763219 (-)	399	WP_004255236.1	hypothetical protein	-
MXM90_RS08745 (MXM90_08745)	-	1763323..1763886 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
MXM90_RS08750 (MXM90_08750)	-	1764059..1765237 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=677928 MXM90_RS08725 WP_004255240.1 1759719..1760366(-) (comEA) [Lactococcus lactis strain JNU 534]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=677928 MXM90_RS08725 WP_004255240.1 1759719..1760366(-) (comEA) [Lactococcus lactis strain JNU 534]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367