Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   L9B60_RS09535 Genome accession   NZ_CP094975
Coordinates   2130715..2132799 (-) Length   694 a.a.
NCBI ID   WP_249678239.1    Uniprot ID   -
Organism   Pseudomonas abieticivorans strain PIA16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2125715..2137799
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L9B60_RS09520 - 2127414..2129018 (-) 1605 WP_249678236.1 AAA family ATPase -
  L9B60_RS09525 aroB 2129035..2130132 (-) 1098 WP_249678237.1 3-dehydroquinate synthase -
  L9B60_RS09530 aroK 2130192..2130710 (-) 519 WP_249678238.1 shikimate kinase AroK -
  L9B60_RS09535 pilQ 2130715..2132799 (-) 2085 WP_249678239.1 type IV pilus secretin PilQ family protein Machinery gene
  L9B60_RS09540 - 2132834..2133361 (-) 528 WP_249678240.1 pilus assembly protein PilP -
  L9B60_RS09545 pilO 2133358..2133981 (-) 624 WP_249678241.1 type 4a pilus biogenesis protein PilO -
  L9B60_RS09550 - 2133978..2134544 (-) 567 WP_249678242.1 PilN domain-containing protein -
  L9B60_RS09555 comM 2134544..2135608 (-) 1065 WP_249678243.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 74963.12 Da        Isoelectric Point: 5.1062

>NTDB_id=674644 L9B60_RS09535 WP_249678239.1 2130715..2132799(-) (pilQ) [Pseudomonas abieticivorans strain PIA16]
MNRILSAFGISLWIALVSPIVAATTLKTLDVAALPGDRVELKLGFDGPAIAPRGYTTEQPARIALDMPGVVNRLASKTRD
LGVGNARGLVVVEAKDRTRLIINLTNLAPYSSRVDGNNLYVMIGQGAKELQGATAAPARTLPAASAPVAFKAPVPPGRAI
RNVDFQRGELGEGNVVIDLTDPSISPDIQEQGGQIRVNFNKTQLPEALRVRLDVKDFATPVQFVNSSASGDNASIVIEPV
GAFDYSAYQTENKLTISVRPLTNEDVTRRNAEHFVYTGEKLSLNFQDIDVRSVLQLIADFTHLNLVASDTVQGGITLRLQ
NVPWDQALDLILKTKGLDKRKVGNVLLVAPAEEIAARERQELESQKQIQELAPLRRELLQVNYAKAADIAKLFQSVTNSG
KAGKTEERGSITVDDRTNSIIAYQTQESLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGQSNTSGNSNFT
AYGGSSTTSTTTAAGTTTPFVDLGASNATSGIGLGFVTNNVLLDLELTAMEKTGNGEVVSQPKVVTSDKETAKILKGTEV
PYQESSSSGATSVMFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDYLNEVLGQPPIKKNEVNAKVLINDGETIVIGGVF
SNTQTKTVEKVPFLGDVPYVGRLFRNDTVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=674644 L9B60_RS09535 WP_249678239.1 2130715..2132799(-) (pilQ) [Pseudomonas abieticivorans strain PIA16]
ATGAACAGGATTCTTTCAGCCTTCGGCATTTCGCTATGGATAGCGCTGGTTTCGCCGATTGTCGCGGCGACGACGCTCAA
GACCCTGGACGTCGCGGCGCTGCCCGGTGATCGGGTCGAGCTCAAGCTGGGCTTTGACGGCCCGGCCATCGCGCCGCGTG
GCTACACCACCGAGCAGCCGGCGCGTATTGCCCTGGACATGCCCGGCGTGGTCAACCGCCTGGCCAGCAAGACCCGTGAC
CTGGGCGTGGGCAATGCCCGCGGCCTGGTGGTGGTCGAGGCCAAGGATCGTACCCGGCTGATCATTAACCTGACCAACCT
TGCGCCCTATAGCTCGCGGGTCGATGGCAACAACCTCTATGTGATGATCGGCCAAGGTGCCAAGGAACTGCAGGGTGCCA
CTGCGGCCCCGGCTCGCACTTTGCCTGCTGCGTCAGCGCCCGTTGCGTTCAAGGCGCCCGTGCCGCCTGGCCGGGCCATC
CGCAATGTCGACTTCCAGCGGGGCGAACTGGGCGAGGGCAACGTCGTCATCGACCTGACCGACCCGTCCATCAGTCCGGA
TATCCAGGAGCAGGGCGGGCAGATCCGCGTCAACTTCAACAAGACCCAACTGCCGGAAGCGCTGCGCGTGCGGCTGGATG
TGAAGGATTTCGCCACGCCGGTGCAGTTCGTCAACTCCAGCGCATCGGGTGACAACGCCAGCATCGTCATCGAGCCGGTG
GGCGCGTTCGACTACTCCGCCTACCAGACTGAGAACAAGCTGACCATCAGCGTGCGCCCGCTGACCAACGAAGACGTGAC
CCGCCGCAATGCCGAGCATTTCGTGTACACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGCGCTCGGTGC
TGCAACTGATCGCCGACTTCACCCACTTGAACCTGGTGGCCAGTGACACCGTGCAGGGTGGCATCACTTTACGCCTGCAA
AACGTGCCTTGGGACCAGGCGCTGGACCTGATCTTGAAAACCAAGGGGCTGGACAAGCGCAAGGTTGGCAACGTGTTGTT
GGTGGCGCCGGCTGAAGAGATCGCCGCGCGCGAACGCCAGGAGCTGGAGTCGCAAAAGCAGATCCAGGAGCTGGCGCCGC
TGCGTCGGGAATTGCTGCAGGTGAACTACGCTAAGGCGGCCGACATCGCCAAGCTGTTCCAGTCGGTGACCAATTCCGGC
AAGGCGGGTAAAACCGAGGAGCGTGGGTCGATCACCGTCGATGATCGCACCAACAGCATCATCGCCTATCAAACCCAGGA
AAGCCTGGACGAACTGCGCCGCATCGTCGCCCAACTGGACATCCCGGTGCGCCAGGTGATGATCGAGGCGCGTATCGTGG
AGGCCAACGTCGATTACGACAAGAGCCTGGGCGTGCGTTGGGGCGGCCAGAGCAATACCTCCGGCAACAGTAATTTCACT
GCGTACGGTGGCAGCAGCACCACGAGCACCACCACCGCAGCCGGTACCACCACGCCGTTCGTCGACCTGGGGGCTTCCAA
TGCTACCTCCGGCATTGGCCTGGGCTTCGTGACCAACAACGTATTGCTGGACCTTGAACTGACGGCCATGGAAAAAACCG
GTAACGGCGAGGTGGTGTCGCAACCCAAGGTGGTGACCTCCGACAAAGAGACCGCCAAGATCCTCAAGGGCACCGAAGTG
CCGTACCAGGAGTCCAGCTCCAGCGGCGCCACGTCGGTGATGTTCAAGGAGGCTGCGCTGTCGCTGGAGGTGACCCCGCA
GATCACCCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGATGAGCCTGACTACCTGAATGAAGTGCTTGGGC
AGCCGCCGATCAAAAAGAACGAGGTGAACGCCAAAGTCCTGATCAATGACGGTGAAACCATAGTGATTGGTGGGGTATTC
TCCAACACCCAAACAAAAACTGTAGAAAAGGTGCCATTTTTGGGCGATGTGCCTTATGTTGGCCGCCTTTTCCGCAACGA
TACTGTGTCGGAAGCAAAATCCGAGCTGTTGGTATTCTTGACACCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTC
GTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(378-445)

Secretin and TonB N terminus short domain (STN)

(304-350)

Bacterial type II and III secretion system protein (Secretin)

(529-685)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.967

100

0.744
  comQ Acinetobacter baylyi ADP1

41.77

100

0.435
  pilQ Acinetobacter baumannii strain A118

38.972

100

0.415
  pilQ Acinetobacter baumannii D1279779

38.972

100

0.415
  pilQ Legionella pneumophila strain Lp02

39.086

97.695

0.382
  pilQ Legionella pneumophila strain ERS1305867

39.086

97.695

0.382