Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KF707C_RS26545 Genome accession   NZ_AP014862
Coordinates   5728835..5730946 (-) Length   703 a.a.
NCBI ID   WP_036993218.1    Uniprot ID   -
Organism   Metapseudomonas furukawaii strain KF707     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5723835..5735946
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KF707C_RS26530 (KF707C_52850) - 5725509..5727146 (-) 1638 WP_003453183.1 AAA family ATPase -
  KF707C_RS26535 (KF707C_52860) aroB 5727156..5728259 (-) 1104 WP_003453184.1 3-dehydroquinate synthase -
  KF707C_RS26540 (KF707C_52880) aroK 5728311..5728829 (-) 519 WP_081608093.1 shikimate kinase AroK -
  KF707C_RS26545 (KF707C_52890) pilQ 5728835..5730946 (-) 2112 WP_036993218.1 type IV pilus secretin PilQ family protein Machinery gene
  KF707C_RS26550 (KF707C_52900) pilP 5731001..5731537 (-) 537 WP_003453191.1 type 4a pilus biogenesis lipoprotein PilP -
  KF707C_RS26555 (KF707C_52910) pilO 5731534..5732157 (-) 624 WP_003453193.1 type 4a pilus biogenesis protein PilO -
  KF707C_RS26560 (KF707C_52920) pilN 5732154..5732774 (-) 621 WP_003453194.1 type 4a pilus biogenesis protein PilN -
  KF707C_RS26565 (KF707C_52930) pilM 5732774..5733838 (-) 1065 WP_036993220.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 703 a.a.        Molecular weight: 75398.70 Da        Isoelectric Point: 4.9319

>NTDB_id=67153 KF707C_RS26545 WP_036993218.1 5728835..5730946(-) (pilQ) [Metapseudomonas furukawaii strain KF707]
MKSSLSRLGITLLAALLSPALLAADLQSLDVAALPGDRVELKLAFDEPVVAPRGYTIEQPARIALDLPGVSNKLGTKNRE
LGVGNARSLTVVEAKDRTRLIINLSTLVPYSTRVEGRNLYVLVGESASAPAAPAAPRPVAAAPAPVAAPLSAAQPAKSYA
RVGRTIENIDFQRGEQGEGNVVITLSDPSVSPDIQEQGGRIRLNFAKTQLPEPLRVRLDVKDFATPVQFVSAAGSGNDAS
ISIEPTGFYDYLAYQTDNKLTVSIKPLTQDDVEKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGN
ITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQ
SVTSGDPSAAGTDERGSITVDDRTNSIIAYQTQDKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVNWRGASVG
DNNFVIGGGGNGRPAAGNANNPLLANAPFVDLGATGATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKET
AKILKGAEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFGNALNGVPPINKNEVNAKVLVNDG
ETIVIGGVFSNTQTKAVDKVPFLGDLPFLGRMFRRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2112 bp        

>NTDB_id=67153 KF707C_RS26545 WP_036993218.1 5728835..5730946(-) (pilQ) [Metapseudomonas furukawaii strain KF707]
ATGAAAAGCTCGCTATCGCGCCTTGGTATCACGCTCTTGGCCGCCCTGCTGTCGCCGGCGCTGCTGGCGGCTGACTTGCA
GTCCCTGGATGTCGCGGCCCTGCCGGGCGACAGGGTCGAACTCAAGCTGGCCTTCGACGAGCCGGTGGTGGCGCCGCGCG
GATACACCATCGAGCAGCCGGCGCGCATAGCCCTGGACCTGCCTGGGGTGTCCAACAAGCTGGGCACGAAGAACCGCGAA
CTGGGCGTTGGCAATGCCCGCAGCCTGACCGTGGTGGAGGCCAAGGATCGCACTCGACTGATCATCAACCTGTCCACACT
CGTGCCTTACAGTACGCGCGTGGAGGGTCGTAACCTCTATGTGCTGGTGGGTGAGTCCGCCTCGGCTCCGGCGGCGCCCG
CCGCACCACGTCCCGTCGCCGCCGCGCCGGCGCCCGTGGCTGCGCCCCTGAGCGCCGCGCAGCCGGCCAAGAGCTATGCC
CGCGTCGGACGTACCATCGAGAACATCGATTTCCAGCGTGGAGAGCAGGGTGAGGGCAATGTAGTCATCACCCTGTCCGA
TCCGAGCGTGAGCCCGGACATCCAGGAGCAGGGCGGTCGCATCCGCCTCAACTTCGCCAAGACCCAGTTGCCGGAACCCT
TGCGTGTCCGTCTGGATGTGAAGGACTTCGCCACCCCGGTGCAGTTCGTCAGTGCCGCCGGTTCGGGCAATGACGCCAGC
ATCAGCATCGAGCCCACCGGCTTCTACGATTACCTGGCGTACCAGACCGACAACAAGCTGACCGTCAGCATCAAGCCGCT
GACCCAGGATGACGTCGAGAAGCGCAAGGCCGAGCGTTTCGCCTACTCGGGTGAAAAGCTCTCCCTGAACTTCCAGGATA
TCGACGTTCGCTCGGTGCTTCAGCTGATCGCCGATTTCACTGACCTGAACCTGGTGGCGTCCGATACAGTGCAGGGCAAT
ATCACCCTGCGGCTGCAGAACGTGCCCTGGGATCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAA
GGTGGGCAATGTGCTCCTGGTGGCGCCGGCTGACGAGATCGCGGCCCGCGAGCGCCAGGAACTGGAATCGCAGAAGCAGA
TCGCAGAACTGGCTCCCCTGCGCCGTGAGCTGATTCAGGTGAACTATGCCAAGGCGGCTGACATCGCCAAGCTGTTCCAG
TCGGTCACCAGTGGCGATCCGAGTGCCGCCGGCACCGATGAGCGCGGCTCCATCACCGTGGATGACCGGACCAACAGCAT
CATCGCCTACCAGACCCAGGACAAGCTGGACGAGCTGCGCCGTATCGTCACCCAACTGGATATTCCCGTCCGCCAGGTGA
TGATCGAGGCCCGCATCGTCGAGGCCAACGTGGACTACGACAAGGCGCTGGGCGTGAATTGGCGCGGTGCCAGCGTCGGT
GACAACAACTTCGTGATCGGCGGCGGCGGCAATGGCCGTCCGGCGGCGGGTAACGCCAACAACCCGCTGCTGGCCAACGC
CCCGTTCGTCGACCTGGGTGCCACCGGGGCCACTTCCAGCATCGGTATCGGCTTCATCACCGACAACACCATCCTTGACC
TTGAACTGTCGGCGATGGAGAAAACCGGTAATGGCGAGATCGTTTCTCAGCCCAAGGTGGTCACTTCCGACAAGGAGACT
GCAAAGATCCTGAAGGGCGCCGAGATTCCCTACCAGGAGGCGAGCTCCAGTGGCGCCACCAGCACCTCCTTCAAGGAAGC
CGCGCTGTCCCTGGAGGTCACTCCGCAGATCACGCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGATGCGC
CGGACTTCGGCAACGCCCTGAACGGTGTACCGCCCATCAACAAGAACGAGGTTAACGCCAAGGTACTGGTGAACGATGGT
GAAACCATCGTGATAGGTGGGGTATTCTCCAACACCCAAACCAAGGCGGTGGACAAGGTGCCCTTCCTCGGCGACCTGCC
CTTCCTCGGCCGCATGTTCCGTCGCGACGTGGTTCAGGACAAGAAATCCGAGCTCCTGGTATTCCTCACCCCCCGCATCA
TGAATAACCAGGCCATTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(384-452)

AMIN domain (AMIN)

(38-119)

Bacterial type II and III secretion system protein (Secretin)

(538-694)

Secretin and TonB N terminus short domain (STN)

(310-356)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

78.025

100

0.798
  comQ Acinetobacter baylyi ADP1

43.224

99.716

0.431
  pilQ Acinetobacter baumannii strain A118

40.762

100

0.411
  pilQ Acinetobacter baumannii D1279779

40.762

100

0.411
  pilQ Legionella pneumophila strain Lp02

39.239

97.155

0.381
  pilQ Legionella pneumophila strain ERS1305867

39.092

97.155

0.38

Multiple sequence alignment