Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MST18_RS01320 Genome accession   NZ_CP094555
Coordinates   280186..281895 (-) Length   569 a.a.
NCBI ID   WP_180133246.1    Uniprot ID   -
Organism   Acinetobacter sp. YH12068_T     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 275186..286895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MST18_RS01290 (MST18_01290) - 275254..275577 (+) 324 WP_067763086.1 pyrimidine/purine nucleoside phosphorylase -
  MST18_RS01295 (MST18_01295) rlmB 275753..276511 (+) 759 WP_180133250.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MST18_RS01300 (MST18_01300) - 276520..277440 (+) 921 WP_373686666.1 DMT family transporter -
  MST18_RS01305 (MST18_01305) coaE 277455..278057 (-) 603 WP_180133248.1 dephospho-CoA kinase -
  MST18_RS01310 (MST18_01310) pilD 278067..278927 (-) 861 WP_180133247.1 A24 family peptidase Machinery gene
  MST18_RS01315 (MST18_01315) pilC 278927..280153 (-) 1227 WP_078192563.1 type II secretion system F family protein Machinery gene
  MST18_RS01320 (MST18_01320) pilB 280186..281895 (-) 1710 WP_180133246.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MST18_RS01325 (MST18_01325) tpiA 282171..282962 (+) 792 WP_180133245.1 triose-phosphate isomerase -
  MST18_RS01330 (MST18_01330) secG 282976..283305 (+) 330 WP_078192560.1 preprotein translocase subunit SecG -
  MST18_RS01360 (MST18_01360) rimP 284236..284760 (+) 525 WP_078192559.1 ribosome maturation factor RimP -
  MST18_RS01365 (MST18_01365) nusA 284796..286280 (+) 1485 WP_078192558.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 63617.57 Da        Isoelectric Point: 4.7954

>NTDB_id=670161 MST18_RS01320 WP_180133246.1 280186..281895(-) (pilB) [Acinetobacter sp. YH12068_T]
MTAVQGSSRFTGFIRRLVDEGHLSAEKMQAALQNSKKAHQDIVPHLIQEYRLSARLIAETISIEFGEPVFDISTYDTSLI
IRDEFEDKLITKHRILPIFKHGNLLFVAISNPTNIEAVDAIRFGSKLNTETIIVEHDKLEKLIEQNFAEESTFEFDDDFD
LDLDSETPDPSQEDEDEVTGDEAPIVKYINKLLVDAIRMGASDLHFEPYEKSYRVRYRVDGVLRTITNPPLQLATRLASR
LKVMSQMDISEKRIPQDGRIKLKLSKNKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEPDQKELFMQALEKPQ
GMLLITGPTGSGKTVSLYTGLNILNREDTNISTAEDPVEINLEGINQVNVNNKVGLTFSAALKSFLRQDPDIVMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSQCKETVDVPKQSLLELGFT
EDDLNTPEFQIYQPVGCAECREGYKGRVGIYEVMKVTPEISRIIMEDGNALQIAEASNQAGFNNLRRSGLIKVMQGVTSL
QEVNRVTSE

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=670161 MST18_RS01320 WP_180133246.1 280186..281895(-) (pilB) [Acinetobacter sp. YH12068_T]
ATGACAGCAGTTCAAGGTTCTTCAAGATTTACGGGGTTTATTCGTCGCTTAGTTGACGAAGGGCATCTGTCTGCCGAAAA
AATGCAAGCAGCGCTGCAAAATTCAAAAAAGGCTCATCAAGATATCGTGCCGCATTTGATTCAGGAATATCGCCTTTCAG
CACGCCTGATCGCAGAAACCATCTCGATAGAGTTCGGTGAACCGGTTTTTGATATTTCAACTTATGATACGTCTTTAATT
ATACGTGATGAATTTGAAGATAAACTCATTACCAAGCATCGTATTTTGCCTATTTTTAAACATGGCAATTTATTATTCGT
TGCGATCAGTAATCCCACCAATATTGAAGCAGTAGATGCCATCCGATTTGGTAGTAAGCTAAATACCGAAACAATTATTG
TTGAACATGACAAACTTGAAAAGTTAATTGAGCAGAACTTCGCTGAAGAAAGTACTTTTGAGTTTGATGATGACTTTGAT
CTTGATTTAGATAGTGAAACACCCGATCCATCTCAAGAAGATGAAGATGAGGTAACGGGTGATGAAGCGCCGATTGTTAA
GTACATCAATAAACTACTAGTCGACGCCATTCGCATGGGTGCATCCGACTTACATTTCGAACCCTATGAAAAGTCTTACC
GCGTCCGCTACCGTGTCGATGGCGTGTTAAGAACAATTACTAATCCGCCCTTGCAGCTCGCGACTCGACTTGCTTCGCGT
TTAAAAGTCATGTCACAAATGGACATCTCGGAAAAGCGTATACCTCAAGACGGTCGTATCAAGTTAAAACTGTCAAAGAA
CAAAGCTATCGACTTCCGTGTGAACTCTTTACCGACATTGTTTGGTGAAAAACTTGTTCTACGTATCCTTGATCCATCTA
GTGCGATGCTAGGAATTGATGCGTTGGGTTATGAACCTGATCAAAAAGAACTGTTCATGCAGGCTTTAGAAAAACCACAA
GGCATGCTGCTCATTACCGGTCCAACGGGTTCCGGTAAAACCGTATCTCTATATACAGGTCTAAATATTCTCAACCGTGA
AGATACCAACATTTCAACGGCCGAGGATCCTGTGGAAATTAACTTAGAAGGGATCAATCAGGTCAACGTAAATAATAAAG
TCGGCCTCACCTTCTCTGCAGCCTTAAAATCATTCTTACGTCAAGACCCTGATATTGTGATGGTCGGTGAGATTCGAGAT
TTAGAAACGGCTGAAATAGCAATTAAAGCGGCTCAAACAGGTCACATGGTGATGTCTACACTGCACACCAATAGTGCACC
TGAAACACTGACACGCTTACGCAATATGGGCGTGCCTTCATTTAACATCGCGACATCCGTAAACCTTGTCATTGCGCAGC
GACTTGCACGACGCTTATGTTCACAATGTAAAGAAACAGTAGACGTACCAAAACAAAGTCTATTGGAGCTTGGTTTTACC
GAAGATGATTTAAATACACCAGAATTCCAAATATACCAGCCTGTGGGTTGTGCCGAATGCCGTGAAGGCTATAAAGGTCG
TGTGGGTATTTACGAAGTCATGAAAGTGACACCTGAAATTTCAAGAATTATTATGGAAGACGGCAATGCCTTACAAATTG
CCGAAGCTTCAAATCAAGCAGGTTTCAACAATTTACGCCGTTCAGGCTTAATTAAGGTCATGCAGGGGGTGACTTCACTT
CAAGAAGTCAATCGTGTGACCAGTGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

79.474

100

0.796
  pilB Acinetobacter baylyi ADP1

74.912

100

0.75
  pilB Legionella pneumophila strain ERS1305867

53.846

93.673

0.504
  pilF Neisseria gonorrhoeae MS11

48.932

98.77

0.483
  pilB Vibrio cholerae strain A1552

49.638

97.012

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

49.903

90.861

0.453
  pilB Vibrio campbellii strain DS40M4

49.13

90.861

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

33.923

100

0.371
  pilF Thermus thermophilus HB27

37.004

97.364

0.36