Detailed information NTG0669808

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: MTO79_RS10325
• Genome accession: NZ_CP094474
• Coordinates: 2048393..2049040 (-)
• Length: 215 a.a.
• NCBI ID: WP_004255240.1
• Uniprot ID: A0A1V0NHR8
• Organism: Lactococcus lactis strain SCB469
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2043393..2054040

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MTO79_RS10300 (MTO79_10300)	atpF	2043464..2043970 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
MTO79_RS10305 (MTO79_10305)	atpB	2043985..2044698 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
MTO79_RS10310 (MTO79_10310)	-	2044743..2044958 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
MTO79_RS10315 (MTO79_10315)	-	2045142..2045918 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
MTO79_RS10320 (MTO79_10320)	-	2046203..2048412 (-)	2210	Protein_1993	DNA internalization-related competence protein ComEC/Rec2	-
MTO79_RS10325 (MTO79_10325)	comEA	2048393..2049040 (-)	648	WP_004255240.1	ComEA family DNA-binding protein	Machinery gene
MTO79_RS10330 (MTO79_10330)	-	2049100..2050461 (-)	1362	WP_058221762.1	ABC transporter permease	-
MTO79_RS10335 (MTO79_10335)	-	2050458..2051390 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
MTO79_RS10340 (MTO79_10340)	-	2051495..2051893 (-)	399	WP_004255236.1	hypothetical protein	-
MTO79_RS10345 (MTO79_10345)	-	2051997..2052560 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
MTO79_RS10350 (MTO79_10350)	-	2052733..2053911 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23651.83 Da
• Isoelectric Point: 5.0062

>NTDB_id=669808 MTO79_RS10325 WP_004255240.1 2048393..2049040(-) (comEA) [Lactococcus lactis strain SCB469]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKNGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=669808 MTO79_RS10325 WP_004255240.1 2048393..2049040(-) (comEA) [Lactococcus lactis strain SCB469]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAAATGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (70-125)

• Helix-hairpin-helix motif (HHH_3) (151-212)

3D structure

• AlphaFold DB: A0A1V0NHR8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.688	100	0.767
comEA/celA/cilE	Streptococcus mitis SK321	44.907	100	0.451
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.578	100	0.442
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae D39	41.553	100	0.423
comEA/celA/cilE	Streptococcus pneumoniae R6	41.553	100	0.423
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.743	100	0.423
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367