Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MRY16_RS13510 Genome accession   NZ_CP094445
Coordinates   2904432..2906156 (+) Length   574 a.a.
NCBI ID   WP_099949291.1    Uniprot ID   -
Organism   Acinetobacter junii strain A2-1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2899432..2911156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MRY16_RS13475 (MRY16_13475) nusA 2900284..2901768 (-) 1485 WP_004916840.1 transcription termination factor NusA -
  MRY16_RS13480 (MRY16_13480) rimP 2901806..2902330 (-) 525 WP_004916844.1 ribosome maturation factor RimP -
  MRY16_RS13500 (MRY16_13500) secG 2902953..2903282 (-) 330 WP_004916847.1 preprotein translocase subunit SecG -
  MRY16_RS13505 (MRY16_13505) tpiA 2903295..2904086 (-) 792 WP_004958617.1 triose-phosphate isomerase -
  MRY16_RS13510 (MRY16_13510) pilB 2904432..2906156 (+) 1725 WP_099949291.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MRY16_RS13515 (MRY16_13515) pilC 2906181..2907410 (+) 1230 WP_243483445.1 type II secretion system F family protein Machinery gene
  MRY16_RS13520 (MRY16_13520) pilD 2907423..2908283 (+) 861 WP_099949293.1 A24 family peptidase Machinery gene
  MRY16_RS13525 (MRY16_13525) coaE 2908285..2908884 (+) 600 WP_004957489.1 dephospho-CoA kinase -
  MRY16_RS13530 (MRY16_13530) - 2908881..2909792 (-) 912 WP_243483447.1 DMT family transporter -
  MRY16_RS13535 (MRY16_13535) rlmB 2909823..2910572 (-) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  MRY16_RS13540 (MRY16_13540) - 2910701..2911027 (-) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63650.78 Da        Isoelectric Point: 5.0662

>NTDB_id=669549 MRY16_RS13510 WP_099949291.1 2904432..2906156(+) (pilB) [Acinetobacter junii strain A2-1]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVASTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LDKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=669549 MRY16_RS13510 WP_099949291.1 2904432..2906156(+) (pilB) [Acinetobacter junii strain A2-1]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTTGATTTTGGGGAAAATGACGAC
TTTGACTTAGATAATGTTGATGTTGCATCGACTGATACAAACAATACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCCCCTATCGTCAAATATATCAACAAGCTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTCCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TTTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTATTTATGAGTGCA
TTAGACAAGCCTCAGGGTATGCTTCTGATTACTGGTCCGACAGGTTCTGGTAAAACCGTATCGTTATATACAGGTTTAAA
TATCTTAAACACAGAAGATACCAATATTTCGACCGCAGAAGACCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGCTTAACTTTCTCAGCGGCGTTAAAAGCATTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAAGCAGCTCAAACAGGTCACATGGTGATGTCAACCCTGCA
TACCAATAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCATCTTTCAATATTGCAACTTCTGTTAATC
TAGTGATTGCTCAACGTTTAGCTCGTCGTCTATGCTCGGAATGCAAACAACCTATTGAGATTCCCAAGCAAAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCCAACCCTGACTTTCAAATTTTCCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAGGTCATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCATTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTTAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.391

100

0.815
  pilB Acinetobacter baylyi ADP1

76.696

100

0.768
  pilB Legionella pneumophila strain ERS1305867

52.974

93.728

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilF Neisseria gonorrhoeae MS11

50.457

95.296

0.481
  pilB Vibrio cholerae strain A1552

49.097

96.516

0.474
  pilB Vibrio campbellii strain DS40M4

51.823

90.767

0.47
  pilB/pilB1 Synechocystis sp. PCC 6803

34.903

100

0.375

Multiple sequence alignment