Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   IEQ11_RS19575 Genome accession   NZ_CP094357
Coordinates   4775258..4776985 (+) Length   575 a.a.
NCBI ID   WP_191822051.1    Uniprot ID   -
Organism   Lysobacter capsici strain VKM B-2533     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4770258..4781985
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IEQ11_RS19555 (IEQ11_19555) - 4771530..4772690 (-) 1161 WP_191822047.1 hypothetical protein -
  IEQ11_RS19560 (IEQ11_19560) - 4772687..4773547 (-) 861 WP_191822048.1 hypothetical protein -
  IEQ11_RS19565 (IEQ11_19565) - 4773537..4774238 (-) 702 WP_191822049.1 class I SAM-dependent methyltransferase -
  IEQ11_RS19570 (IEQ11_19570) - 4774229..4775122 (-) 894 WP_191822050.1 glycosyltransferase family 2 protein -
  IEQ11_RS19575 (IEQ11_19575) pilB 4775258..4776985 (+) 1728 WP_191822051.1 type IV-A pilus assembly ATPase PilB Machinery gene
  IEQ11_RS19580 (IEQ11_19580) pilC 4776989..4778245 (+) 1257 WP_191822052.1 type II secretion system F family protein Machinery gene
  IEQ11_RS19585 (IEQ11_19585) - 4778267..4779154 (+) 888 WP_191822053.1 prepilin peptidase -
  IEQ11_RS19590 (IEQ11_19590) coaE 4779158..4779778 (+) 621 WP_191822054.1 dephospho-CoA kinase -
  IEQ11_RS19595 (IEQ11_19595) - 4779929..4780903 (-) 975 WP_247024614.1 Nudix family hydrolase -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62909.41 Da        Isoelectric Point: 6.3668

>NTDB_id=669223 IEQ11_RS19575 WP_191822051.1 4775258..4776985(+) (pilB) [Lysobacter capsici strain VKM B-2533]
MSTVATANLVGITGIARRLVMDGALDEVRAREAMTAASAERKPLASYLAEHRLATPAQLAAANSIEFGVPLFDPSTMDPG
QAPIRAISEELIRKHNALPLFKRGNKLFIGLADPTNTKALDEIKFQTNAAVEAILVDEELIRRTLDRWLETSDALGDAVG
DGEGLDNLEIGKDDDLNTTSDTGIDAKGDDTPVVKFINKVLVDAIRRGASDIHFEPYETDYRVRLRIDGLLKQVAKVPNK
LHPRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRMSTLPTLFGEKIVLRILDGSAARLGIEKLGYEDYQKELF
VNAVVKPYGMVLVTGPTGSGKTVSLYTALNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDV
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVTLVIAQRLARRLHDCKREVHLPEHA
LLAEGFTHEEIASGFKLYEPVGCPDCTEGYKGRTGIYQVMPMTDEIQAIVLEGGNAMQIAAAAIRSGVPDLRRSALIKVM
NGVTGLAEINRVTKD

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=669223 IEQ11_RS19575 WP_191822051.1 4775258..4776985(+) (pilB) [Lysobacter capsici strain VKM B-2533]
ATGTCCACTGTCGCGACCGCCAACTTGGTGGGAATCACCGGCATTGCCCGGCGTTTGGTCATGGATGGGGCTCTGGACGA
GGTCAGGGCGCGCGAAGCCATGACCGCCGCCAGCGCCGAACGCAAGCCGCTGGCCTCCTATCTGGCCGAACACCGCCTGG
CCACTCCGGCCCAGCTCGCCGCCGCCAACTCGATCGAGTTCGGCGTGCCGCTGTTCGACCCGAGCACCATGGATCCGGGC
CAGGCCCCGATCCGGGCGATCAGCGAAGAGCTGATCCGCAAGCACAACGCCTTGCCGCTGTTCAAGCGCGGCAACAAGCT
GTTCATCGGCCTGGCCGATCCGACCAACACCAAGGCCCTGGACGAGATCAAGTTCCAGACCAATGCCGCGGTCGAGGCGA
TCTTGGTCGATGAGGAACTGATCCGCCGCACCCTGGACCGCTGGCTGGAGACCTCCGACGCCCTCGGCGACGCGGTCGGC
GACGGCGAGGGCCTGGACAACCTGGAGATCGGCAAGGACGACGACCTCAACACCACCAGCGACACCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTCAAGTTCATCAACAAGGTGCTGGTCGACGCGATCCGCCGCGGCGCGTCCGACATCCATT
TCGAACCCTACGAAACCGACTACCGGGTGCGCCTGCGCATCGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCGAACAAG
CTGCATCCGCGCATCGCCGCGCGCCTGAAGGTCATGGCGCAGCTCGACATCGCCGAGAAGCGGGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCATGAGCACCTTGCCGACCCTGTTCGGCGAGAAGATCG
TGCTGCGTATCCTCGACGGCAGCGCGGCGCGGCTGGGCATCGAAAAGCTCGGCTACGAGGACTATCAGAAGGAATTGTTC
GTCAACGCGGTGGTCAAGCCCTACGGCATGGTGCTGGTCACCGGCCCGACCGGCTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTCAACATCCTCAACGACGAACAACGCAACATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCA
ATCAGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCCGCGCTGCGCAGCTTCCTGCGTCAGGATCCGGACGTG
ATCATGGTCGGCGAAATCCGCGACCTCGAAACCGCCGAGATCGCGATCAAGGCCGCCCAGACCGGCCACATGGTGCTGTC
GACCCTGCACACCAACGACGCGCCGCAGACCATCGCCCGTCTGATGAACATGGGCGTGGCGCCGTACAACATCACCTCGT
CGGTCACCCTGGTGATCGCACAGCGTCTCGCCCGCCGTCTGCACGACTGCAAGCGCGAGGTGCATCTGCCCGAACACGCG
CTGCTCGCCGAGGGGTTCACCCACGAGGAGATCGCGTCGGGTTTCAAGTTGTACGAGCCGGTCGGATGTCCGGACTGTAC
CGAAGGCTACAAGGGACGTACCGGCATCTATCAGGTCATGCCCATGACCGACGAAATCCAGGCCATCGTGCTCGAAGGCG
GCAATGCGATGCAGATCGCCGCCGCGGCGATCCGATCGGGCGTGCCCGATCTACGCCGCTCCGCGCTGATCAAGGTGATG
AACGGCGTCACCGGCCTGGCCGAAATCAACCGCGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.556

98.609

0.548
  pilB Acinetobacter baumannii D1279779

55.36

98.957

0.548
  pilB Legionella pneumophila strain ERS1305867

50.263

99.304

0.499
  pilB Vibrio cholerae strain A1552

49.215

99.652

0.49
  pilF Neisseria gonorrhoeae MS11

47.619

98.609

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

49.617

90.783

0.45
  pilB Vibrio campbellii strain DS40M4

51.738

85.043

0.44
  pilB/pilB1 Synechocystis sp. PCC 6803

35.831

100

0.383
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.242

100

0.376
  pilF Thermus thermophilus HB27

39.698

92

0.365

Multiple sequence alignment