Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PBDP_RS02345 Genome accession   NZ_AP014703
Coordinates   549442..551529 (+) Length   695 a.a.
NCBI ID   WP_212801765.1    Uniprot ID   -
Organism   Pseudomonas sp. St290     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544442..556529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PBDP_RS02325 (PBDP_0459) comM 546654..547718 (+) 1065 WP_109752037.1 pilus assembly protein PilM Machinery gene
  PBDP_RS02330 (PBDP_0460) - 547718..548284 (+) 567 WP_109752038.1 PilN domain-containing protein -
  PBDP_RS02335 (PBDP_0461) pilO 548281..548904 (+) 624 WP_109752039.1 type 4a pilus biogenesis protein PilO -
  PBDP_RS02340 (PBDP_0462) - 548901..549428 (+) 528 WP_109752040.1 pilus assembly protein PilP -
  PBDP_RS02345 (PBDP_0463) pilQ 549442..551529 (+) 2088 WP_212801765.1 type IV pilus secretin PilQ Machinery gene
  PBDP_RS02350 (PBDP_0464) aroK 551534..552052 (+) 519 WP_003196983.1 shikimate kinase AroK -
  PBDP_RS02355 (PBDP_0465) aroB 552201..553301 (+) 1101 WP_212801766.1 3-dehydroquinate synthase -
  PBDP_RS02360 (PBDP_0466) - 553311..554897 (+) 1587 WP_212801767.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74993.06 Da        Isoelectric Point: 5.5467

>NTDB_id=66835 PBDP_RS02345 WP_212801765.1 549442..551529(+) (pilQ) [Pseudomonas sp. St290]
MNRIFSALGISLWIALLSPMVHGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLASKNHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNHLFVVVGKGGSAKSAAAKNTVQAPRTAVASAAPARASAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNSSG
VTTGDSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=66835 PBDP_RS02345 WP_212801765.1 549442..551529(+) (pilQ) [Pseudomonas sp. St290]
ATGAACAGGATTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGGGGCCAGTCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTTACCTTCGATGGTCCGCCGCCGACTCCTCGGG
GCTACACCACCGAACAACCGGCCCGGATTGCCCTGGACCTGCCCGGGGTGACCAATCAACTGGCCAGCAAGAACCACGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACCGTGGTCGAGGCCGGGGATCGCACCCGGTTGATCATCGGCCTTACCCAACT
GGCGCCGTACGATACCCGGGTCGAAGGTAACCATCTGTTCGTGGTGGTCGGCAAGGGGGGCTCTGCGAAGAGCGCTGCTG
CGAAGAACACCGTCCAGGCCCCGCGCACGGCGGTTGCCAGCGCGGCGCCCGCGAGGGCCAGTGCGCCAGTGGGCAAGGCG
ATCCGTGGGGTTGACTTCCAGCGTGGGACCCAGGGCGAAGGCAACGTGGTAATTGATCTGTCTGATCCGTCCATCGCCCC
GGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAGCCATTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAACCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGTCCCATGACCGTCGACGACCT
GCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGCTCGG
TGCTGCAATTGATCGCCGATTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCCTGCGCTTG
CAGAACGTGCCTTGGGACCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGTCTGGACAAGCGCAAGGTCGGCAACGTGCT
GCTGGTGGCTCCGGCCGATGAAATTGCCGCTCGCGAGCGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGACCTGGCAC
CACTGCGGCGTGAGCTGTTGCAGGTCAACTACGCCAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCC
GAGGCGAAAGCCGACGAGCGCGGCACGATCACGGTCGATGAGCGCACCAACAACATCATTGCCTACCAGACCCAGGACCG
GCTCGACGAACTGCGCAGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATCGTCGAGG
CCAATGTCGATTACGACAAAAGCCTGGGTGTGCGTTGGGGCGGTTCGATCCAGAACAAGGGCAACTGGAATTCCTCCGGG
GTCACCACCGGCGACTCCACCACCATTGGCACGCCGGGCAGTACCAGCACCAACCAGCCGTTTGTCGATCTGGGCGCGTC
GGGCAATACCTCCGGAATCGGCATTGCGTTCATTACCGACAACGTCTTGCTGGACCTTGAACTGACGGCCATGGAAAAGA
CCGGCAACGGCGAAATCGTCTCCCAGCCCAAGGTGGTGACCTCCGACAAGGAGACGGCAAAAATCCTCAAGGGCACGGAA
ATCCCTTATCAGGAAGCCAGTTCCAGCGGTGCCACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACTCC
GCAGATCACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACTTGAACAAGGTGCAGG
ATGTGCCGCCAATCAAGAAAAACGAGGTCAACGCCAAGGTCTTGGTCAATGACGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTAGTCTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.727

100

0.748
  comQ Acinetobacter baylyi ADP1

41.667

100

0.417
  pilQ Acinetobacter baumannii strain A118

38.523

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.523

100

0.413
  pilQ Legionella pneumophila strain Lp02

38.382

97.842

0.376
  pilQ Legionella pneumophila strain ERS1305867

38.382

97.842

0.376

Multiple sequence alignment