Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PBDP_RS02325 Genome accession   NZ_AP014703
Coordinates   546654..547718 (+) Length   354 a.a.
NCBI ID   WP_109752037.1    Uniprot ID   -
Organism   Pseudomonas sp. St290     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541654..552718
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PBDP_RS02310 (PBDP_0456) rpmE 541911..542135 (+) 225 WP_003196965.1 50S ribosomal protein L31 -
  PBDP_RS02315 (PBDP_0457) - 542439..543707 (+) 1269 WP_109752035.1 malic enzyme-like NAD(P)-binding protein -
  PBDP_RS02320 (PBDP_0458) - 544002..546446 (-) 2445 WP_178082464.1 penicillin-binding protein 1A -
  PBDP_RS02325 (PBDP_0459) comM 546654..547718 (+) 1065 WP_109752037.1 pilus assembly protein PilM Machinery gene
  PBDP_RS02330 (PBDP_0460) - 547718..548284 (+) 567 WP_109752038.1 PilN domain-containing protein -
  PBDP_RS02335 (PBDP_0461) pilO 548281..548904 (+) 624 WP_109752039.1 type 4a pilus biogenesis protein PilO -
  PBDP_RS02340 (PBDP_0462) - 548901..549428 (+) 528 WP_109752040.1 pilus assembly protein PilP -
  PBDP_RS02345 (PBDP_0463) pilQ 549442..551529 (+) 2088 WP_212801765.1 type IV pilus secretin PilQ Machinery gene
  PBDP_RS02350 (PBDP_0464) aroK 551534..552052 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38067.64 Da        Isoelectric Point: 4.9070

>NTDB_id=66834 PBDP_RS02325 WP_109752037.1 546654..547718(+) (comM) [Pseudomonas sp. St290]
MLRLFNKKAHTLLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVVEKNIADLEGVGQALSRLLAKAKTPVRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=66834 PBDP_RS02325 WP_109752037.1 546654..547718(+) (comM) [Pseudomonas sp. St290]
GTGCTACGACTCTTCAATAAAAAAGCCCATACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTGAAGCTGCTCGAGTT
GAGCCGCCAGGGCGAGCGATATCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCGGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGATCTCGAAGGGGTGGGCCAGGCATTGTCACGGCTGTTGGCCAAGGCCAAGACGCCCGTGCGTAGCGTGGCGGTG
GCCGTTGCGGGGTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGGTTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATTCCCTATCCGCTGGATGAGGTGGCCATCGATTTCGAAGTGGTGGGCGTTTCGCCGC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGACGTGGAGGCTTATGCGCTGGAGCGTGCTTTCGGCTTGCTCGCCACGCAACTGGC
CGCCTCCCAGGAACGGCTGACTGTGGCGGTTATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATTCAGCGCCGCTATGGCTTGACGCCC
GAGCAGGCCGGCTTGGCAAAGAAGCAGGGAGGGCTGCCGGACGATTACCTGAGCGAAGTGTTGCAACCCTTTCGCGAGGC
TCTGGTACAGCAGGTCTCGCGATCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTCGACCACATCCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAACAACGCCTGGGTACCCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGGGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCACCGGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.65

100

0.557
  pilM Acinetobacter baumannii D1279779

55.65

100

0.557
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.305

100

0.483

Multiple sequence alignment