Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MQG37_RS00475 Genome accession   NZ_CP093911
Coordinates   95018..96427 (+) Length   469 a.a.
NCBI ID   WP_005547542.1    Uniprot ID   -
Organism   Aggregatibacter actinomycetemcomitans strain 16R     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 90018..101427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MQG37_RS00455 pnp 90496..92625 (-) 2130 WP_032997542.1 polyribonucleotide nucleotidyltransferase -
  MQG37_RS00460 nanQ 92919..93389 (+) 471 WP_005547536.1 N-acetylneuraminate anomerase -
  MQG37_RS00465 ampD 93863..94417 (-) 555 WP_005567973.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MQG37_RS00470 pilA 94539..94991 (+) 453 WP_005586498.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  MQG37_RS00475 pilB 95018..96427 (+) 1410 WP_005547542.1 GspE/PulE family protein Machinery gene
  MQG37_RS00480 pilC 96420..97643 (+) 1224 WP_005547544.1 type II secretion system F family protein Machinery gene
  MQG37_RS00485 - 97640..98329 (+) 690 WP_005547547.1 A24 family peptidase -
  MQG37_RS00490 coaE 98379..99002 (+) 624 WP_005547548.1 dephospho-CoA kinase -
  MQG37_RS00495 yacG 98992..99204 (+) 213 WP_005547550.1 DNA gyrase inhibitor YacG -
  MQG37_RS00500 - 99204..99476 (+) 273 WP_005547552.1 GNAT family N-acetyltransferase -
  MQG37_RS00505 - 99873..101246 (+) 1374 WP_005547554.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52496.80 Da        Isoelectric Point: 6.1345

>NTDB_id=665407 MQG37_RS00475 WP_005547542.1 95018..96427(+) (pilB) [Aggregatibacter actinomycetemcomitans strain 16R]
MQNPMAQGYLIHSQNHGDFTIAPELWLKNQQQQTVLLRYLALPLAENGQTLWLGLDSLTNLSACEAFSFLTGKTIEPVLI
ESQTLKTALQDLSPRVEKVEENQPLFYSALSQEQPTQPYDEPIIRLLNDIFESATAQKASDIHLEPQADCLQTRFRIDGV
LQVQNTVPLSIASRLISRLKLLAKLDISETRLPQDGRFQFKTTFSDTLDFRLSTLATQFGEKAVLRLQQNRPVQLAFGEL
GMTESQQQRFRRALSQPQGLILVTGPTGSGKSISLYTALQYLNTSEKHIMTAEDPIEIQLPGIIQSQVNHPIGLDFSRLL
RTFLRQDPDIIMLGEIRDEESAAMALRAAQTGHLVLSTLHTNDAPSAISRLQQLGIQCHEIDGSLLLVIAQRLVRKRCQK
CGGNATRFCDCHQGYKGRVGVYQFLQPNLQHPQGYETDFADLGQSALEKLKDGTTDLTEIQRVLGQTHD

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=665407 MQG37_RS00475 WP_005547542.1 95018..96427(+) (pilB) [Aggregatibacter actinomycetemcomitans strain 16R]
ATGCAAAATCCGATGGCGCAAGGTTATTTGATTCACAGCCAAAATCACGGGGACTTTACCATTGCGCCCGAGCTATGGCT
TAAAAATCAGCAACAACAAACCGTCCTGTTGCGTTATCTCGCCCTGCCCTTAGCAGAAAACGGGCAGACCTTGTGGCTAG
GGTTAGACTCGTTAACTAACCTGAGCGCCTGTGAAGCCTTTTCTTTTCTCACCGGCAAAACCATTGAGCCGGTATTAATT
GAAAGCCAAACGCTAAAAACCGCATTGCAGGATTTATCCCCGCGCGTCGAAAAAGTCGAAGAAAATCAACCGCTCTTTTA
TTCCGCACTGTCACAGGAACAGCCGACACAACCGTATGACGAACCGATTATTCGGCTGTTAAATGATATTTTTGAAAGCG
CGACGGCACAAAAAGCCTCCGATATTCATCTGGAACCGCAAGCAGACTGCCTGCAAACCCGTTTTCGCATTGACGGCGTG
CTGCAGGTGCAAAACACCGTTCCCTTATCCATAGCGAGCCGACTGATTTCTCGTTTAAAATTATTGGCAAAACTGGATAT
TAGCGAAACCCGCCTGCCGCAAGACGGACGCTTTCAGTTTAAAACGACGTTTTCCGACACGCTGGATTTTCGCCTTTCCA
CCCTTGCCACCCAATTCGGTGAAAAAGCGGTCCTGCGTTTGCAACAAAATCGTCCCGTGCAACTGGCATTCGGTGAACTG
GGCATGACGGAATCACAGCAACAGCGTTTTCGTCGTGCCTTGAGCCAACCACAAGGCTTGATTTTAGTCACCGGTCCGAC
AGGCAGCGGCAAAAGTATTTCTTTATATACGGCGTTGCAGTACTTAAATACGTCTGAAAAACACATTATGACGGCGGAAG
ATCCCATTGAAATTCAGCTCCCAGGCATTATTCAAAGCCAAGTGAATCACCCTATCGGCTTGGATTTCAGCCGATTGCTT
CGCACCTTTTTACGTCAAGATCCGGACATTATTATGTTAGGTGAAATTCGTGATGAAGAAAGTGCCGCCATGGCATTACG
CGCCGCGCAAACGGGGCATTTGGTGCTTTCGACGCTACATACCAACGACGCCCCTTCCGCCATTTCCCGCCTGCAACAAC
TCGGCATTCAATGCCATGAAATCGACGGCAGTTTGTTGCTGGTGATCGCCCAACGTTTGGTGCGCAAACGCTGCCAAAAG
TGCGGTGGAAATGCCACGCGTTTTTGCGATTGCCATCAAGGCTACAAAGGGCGTGTCGGGGTCTATCAATTCCTGCAACC
GAATTTACAACATCCGCAGGGCTATGAAACGGATTTCGCCGATTTAGGCCAAAGCGCTTTAGAAAAACTCAAAGACGGCA
CAACAGATTTAACCGAAATCCAACGGGTGTTAGGACAAACTCATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(130-396)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(31-93)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

69.427

100

0.697
  pilB Haemophilus influenzae 86-028NP

70.87

98.081

0.695
  pilB Glaesserella parasuis strain SC1401

57.328

98.934

0.567
  pilB Vibrio cholerae strain A1552

40.53

100

0.424
  pilB Acinetobacter baylyi ADP1

39.078

100

0.416
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.412
  pilB Vibrio parahaemolyticus RIMD 2210633

40.041

100

0.412
  pilB Vibrio campbellii strain DS40M4

39.752

100

0.409
  pilB Acinetobacter baumannii D1279779

38.855

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.331

100

0.401
  pilF Neisseria gonorrhoeae MS11

36

100

0.365