Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MOQ58_RS02005 Genome accession   NZ_CP093428
Coordinates   433979..436063 (+) Length   694 a.a.
NCBI ID   WP_280162736.1    Uniprot ID   -
Organism   Pseudomonas migulae strain 8R6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428979..441063
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MOQ58_RS01985 (MOQ58_01985) comM 431191..432255 (+) 1065 WP_008041861.1 pilus assembly protein PilM Machinery gene
  MOQ58_RS01990 (MOQ58_01990) - 432255..432821 (+) 567 WP_008041859.1 PilN domain-containing protein -
  MOQ58_RS01995 (MOQ58_01995) pilO 432818..433441 (+) 624 WP_280162735.1 type 4a pilus biogenesis protein PilO -
  MOQ58_RS02000 (MOQ58_02000) - 433438..433965 (+) 528 WP_008025197.1 pilus assembly protein PilP -
  MOQ58_RS02005 (MOQ58_02005) pilQ 433979..436063 (+) 2085 WP_280162736.1 type IV pilus secretin PilQ Machinery gene
  MOQ58_RS02010 (MOQ58_02010) aroK 436068..436586 (+) 519 WP_032828585.1 shikimate kinase AroK -
  MOQ58_RS02015 (MOQ58_02015) aroB 436738..437838 (+) 1101 WP_008041853.1 3-dehydroquinate synthase -
  MOQ58_RS02020 (MOQ58_02020) - 437849..439456 (+) 1608 WP_253401405.1 AAA family ATPase -

Sequence

Protein


Download         Length: 694 a.a.        Molecular weight: 74954.09 Da        Isoelectric Point: 5.5525

>NTDB_id=664647 MOQ58_RS02005 WP_280162736.1 433979..436063(+) (pilQ) [Pseudomonas migulae strain 8R6]
MNRIFATFGISLWIALLSPMVQAANLKALDVAALPGDRVELKLSFDGAPPAPHGYTTEQPARIALDLPGVVSQLASKTRD
LGGGNARSATVVEAKDRTRLIINLTQLTPYTARVEGNNLFVVVGRGGSNAAAPAARPSRVATATAAPAKAYAPASKAIRG
VDFQRGTQGEGNVVIDLSDPSIAPDIQERDGRIVLNFARTQLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSGA
FDYSTYQTDNKLTVSIRPMTVDDLQKRNAERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQNV
PWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFTSVTSAEAK
VDERGSITVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSLQNKGNWNTSGVSN
GANASSTIGTPGSTSTNSPFVDMGAAANTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEI
PYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVF
SNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSH

Nucleotide


Download         Length: 2085 bp        

>NTDB_id=664647 MOQ58_RS02005 WP_280162736.1 433979..436063(+) (pilQ) [Pseudomonas migulae strain 8R6]
ATGAACAGGATTTTCGCAACCTTCGGTATTTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACAAGCGGCCAACCTCAA
GGCGCTGGATGTTGCCGCGCTGCCGGGAGACCGCGTCGAGTTGAAGTTGTCATTCGATGGCGCGCCGCCGGCGCCCCACG
GTTACACGACGGAGCAGCCTGCGCGGATTGCGCTGGATCTGCCTGGCGTGGTCAGCCAGTTGGCGAGCAAGACCCGTGAT
CTTGGTGGCGGCAACGCCCGCAGCGCCACAGTGGTGGAAGCCAAGGACCGCACGCGTCTGATCATCAACCTGACTCAATT
GACCCCGTACACCGCACGGGTCGAAGGTAACAACCTGTTTGTTGTGGTCGGGCGGGGAGGCAGTAACGCTGCCGCGCCTG
CCGCCAGGCCTTCGCGGGTCGCCACGGCAACTGCGGCGCCGGCCAAGGCTTATGCGCCGGCGAGCAAGGCCATTCGCGGC
GTGGATTTCCAGCGTGGAACCCAGGGCGAAGGCAACGTGGTCATCGACCTGTCGGACCCTTCCATCGCCCCGGACATTCA
GGAGCGCGATGGCAGGATCGTTCTTAATTTTGCCAGAACCCAATTACCAGAACCTTTGCGAGTGCGTCTGGACGTCAAGG
ATTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGTGCCACCGGGGATCGGGCGACCATCAGCATCGAGCCCAGCGGCGCC
TTCGATTATTCGACCTACCAGACCGACAACAAGCTGACCGTCAGCATTCGCCCGATGACCGTCGATGACCTGCAAAAGCG
CAACGCCGAACGTTTTGCCTACACCGGTGAAAAGCTCTCGCTGAACTTCCAGGACATTGACGTGCGCTCGGTGCTGCAAC
TGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCTTGCGTCTGCAAAACGTG
CCATGGGATCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAACGTGTTGCTGGTGGC
GCCGGCCGACGAGATTGCCGCGCGCGAACGTCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGATCTGGCGCCATTGCGTC
GTGAGCTGCTGCAGGTCAATTACGCCAAGGCGGCGGATATCGCCAAGTTGTTCACGTCGGTGACCAGTGCCGAGGCGAAA
GTGGATGAGCGGGGTTCGATCACTGTCGATGACCGGACCAACAACATCATTGCCTATCAGACCCAGGATCGCCTCGACGA
GCTTCGCCGGATCGTCGCGCAACTGGACATCCCGGTGCGTCAGGTGATGATCGAGGCGCGAATCGTAGAAGCCAACGTCG
ACTACGACAAAAGCCTCGGCGTGCGCTGGGGCGGTTCTTTGCAAAACAAAGGCAACTGGAACACGTCCGGGGTCAGCAAC
GGCGCCAATGCTTCGTCGACCATCGGCACGCCCGGCAGCACCAGTACCAACTCGCCGTTCGTCGATATGGGCGCCGCCGC
CAACACCTCGGGGCTCGGCATCGCCTTCATCACCGACAATGTGCTGCTGGATCTTGAGCTGACTGCCATGGAGAAAACCG
GTAACGGCGAAATCGTCTCACAGCCCAAAGTGGTCACGTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATC
CCGTACCAGGAAGCCAGCTCCAGCGGCGCCACATCGGTGTCCTTCAAAGAGGCCTCGCTGTCGCTGGAAGTGACGCCGCA
GATCACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAGGTCCAGGATG
TACCGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTCCTGGTCAATGACGGCGAGACCATCGTAATCGGTGGCGTTTTC
TCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGA
CGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAACAACCAGGCGATTGCTGTGAGTC
ATTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(376-441)

Secretin and TonB N terminus short domain (STN)

(302-348)

Bacterial type II and III secretion system protein (Secretin)

(529-685)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.007

100

0.752
  comQ Acinetobacter baylyi ADP1

41.834

100

0.421
  pilQ Acinetobacter baumannii strain A118

38.247

100

0.415
  pilQ Acinetobacter baumannii D1279779

38.247

100

0.415
  pilQ Legionella pneumophila strain Lp02

38.257

97.55

0.373
  pilQ Legionella pneumophila strain ERS1305867

38.257

97.55

0.373