Detailed information NTG0066384

Bioinformatically predicted

Overview

• Name: comM
• Type: Machinery gene
• Locus tag: AS4_RS20025
• Genome accession: NZ_AP014630
• Coordinates: 4299475..4300533 (-)
• Length: 352 a.a.
• NCBI ID: WP_004816660.1
• Uniprot ID: A0A077L4V4
• Organism: Acinetobacter guillouiae strain NBRC 110550
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4294475..4305533

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AS4_RS20000 (AS4_40370)	aroK	4294787..4295329 (-)	543	WP_004725433.1	shikimate kinase AroK	-
AS4_RS20005 (AS4_40380)	pilQ	4295366..4297540 (-)	2175	WP_096735282.1	type IV pilus secretin PilQ family protein	Machinery gene
AS4_RS20010 (AS4_40390)	pilP	4297561..4298091 (-)	531	WP_096735283.1	pilus assembly protein PilP	Machinery gene
AS4_RS20015 (AS4_40400)	comO	4298088..4298837 (-)	750	WP_096735284.1	type 4a pilus biogenesis protein PilO	Machinery gene
AS4_RS20020 (AS4_40410)	pilN	4298834..4299475 (-)	642	WP_034615056.1	PilN domain-containing protein	Machinery gene
AS4_RS20025 (AS4_40420)	comM	4299475..4300533 (-)	1059	WP_004816660.1	pilus assembly protein PilM	Machinery gene
AS4_RS20030 (AS4_40430)	ponA	4300697..4303237 (+)	2541	WP_096735285.1	penicillin-binding protein PBP1a	-
AS4_RS20035 (AS4_40440)	-	4303298..4304101 (-)	804	WP_096735286.1	putative RNA methyltransferase	-
AS4_RS20040 (AS4_40450)	mraY	4304172..4305290 (-)	1119	WP_004725416.1	phospho-N-acetylmuramoyl-pentapeptide- transferase	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39003.71 Da
• Isoelectric Point: 4.5366

>NTDB_id=66384 AS4_RS20025 WP_004816660.1 4299475..4300533(-) (comM) [Acinetobacter guillouiae strain NBRC 110550]
MLRLYRKPNKGLIGVDISSTTVKILELSVKNGRYWVESYGLSPLLDGSVVEKNILNPEAVADALERAVNIGNPQSQNVAV
AVPTSMVIHKIIEMDADMSDEEREVQIRMDAEQYIPFPLDEVSLDFEVLKDKLANPNRVNVLLVATRAENIDSRVEVLEI
AGLSPKIADVESYAIERAFDVFSDTLPIGVNLVGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTQDVQNRYGLSYDE
AGRAKKDRTLPDDFETEVLMPFLEAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQIDLKKIENDAPSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=66384 AS4_RS20025 WP_004816660.1 4299475..4300533(-) (comM) [Acinetobacter guillouiae strain NBRC 110550]
GTGCTTAGACTATATCGTAAGCCAAATAAGGGGTTAATAGGAGTAGATATTAGTTCTACTACTGTAAAGATTTTAGAGCT
CTCTGTTAAGAACGGTCGTTACTGGGTTGAAAGTTATGGATTAAGTCCACTGCTGGATGGGAGTGTTGTTGAGAAAAACA
TTCTTAATCCTGAAGCAGTTGCTGATGCGTTGGAAAGAGCAGTCAATATTGGCAATCCTCAATCGCAAAATGTCGCGGTT
GCTGTACCTACATCTATGGTGATTCACAAAATCATCGAAATGGATGCAGATATGTCAGATGAGGAAAGAGAAGTTCAGAT
TCGAATGGATGCTGAACAATATATCCCTTTCCCACTGGATGAAGTCAGTCTAGATTTTGAAGTGTTAAAAGATAAGCTGG
CAAATCCAAATCGAGTCAATGTGTTACTTGTTGCAACACGTGCAGAGAATATCGATTCTCGTGTTGAAGTTTTAGAAATC
GCGGGCTTATCACCTAAAATCGCAGATGTAGAAAGTTATGCAATTGAGCGTGCTTTTGATGTCTTTTCCGATACCTTGCC
AATTGGTGTAAATCTTGTGGGTATCCTAGATATTGGTCATACCATGACCACTTTATCTGTGATGCAAAATGGCAAGATCA
TTTATACCCGAGAGCAAGTTTTTGGTGGCAAGCAGCTGACTCAAGATGTACAGAACCGTTATGGTTTGTCTTATGACGAA
GCTGGTCGTGCCAAAAAAGATCGGACTTTACCTGACGATTTTGAAACAGAAGTCTTGATGCCGTTTTTAGAAGCAGTTGT
ACAACAAGCTGCTCGCTCATTACAGTTCTTCTTTTCATCTTCTCAGTTTAACGAGATCGACCATATTTTATTGGCGGGTG
GTAATGCCAATATTCCTGGTTTGGCAAAATTATTACAACAAAAACTCGGTTATCGCGTCACGATTGCAAATCCTTTCTTG
CAAATGGGGTTTTCACCACAAATTGACTTAAAAAAAATAGAAAATGATGCACCTTCTTTAATGGTTGCATGTGGTTTAGC
TTTGAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: A0A077L4V4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comM	Acinetobacter nosocomialis M2	84.375	100	0.844
pilM	Acinetobacter baumannii D1279779	84.091	100	0.841
comM	Acinetobacter baylyi ADP1	81.25	100	0.813
pilM	Legionella pneumophila strain ERS1305867	42.535	100	0.429