Detailed information NTG0663831

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: MNR01_RS14725
• Genome accession: NZ_CP093341
• Coordinates: 3277852..3279576 (-)
• Length: 574 a.a.
• NCBI ID: WP_241918507.1
• Uniprot ID: -
• Organism: Lysobacter sp. S4-A87
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3279747..3297388	3277852..3279576	flank	171

Gene organization within MGE regions

Location: 3277852..3297388

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MNR01_RS14725 (MNR01_14725)	pilB	3277852..3279576 (-)	1725	WP_241918507.1	type IV-A pilus assembly ATPase PilB	Machinery gene
MNR01_RS14730 (MNR01_14730)	-	3279747..3280604 (+)	858	WP_241918508.1	glycosyltransferase family 2 protein	-
MNR01_RS14735 (MNR01_14735)	-	3280604..3281332 (+)	729	WP_241918509.1	class I SAM-dependent methyltransferase	-
MNR01_RS14740 (MNR01_14740)	-	3281272..3282156 (+)	885	WP_241918510.1	class I SAM-dependent methyltransferase	-
MNR01_RS14745 (MNR01_14745)	-	3282171..3283124 (+)	954	WP_241918511.1	hypothetical protein	-
MNR01_RS14750 (MNR01_14750)	-	3283152..3284297 (-)	1146	WP_241918512.1	glycosyltransferase family 4 protein	-
MNR01_RS14755 (MNR01_14755)	pilE	3284365..3284784 (-)	420	WP_241918513.1	pilin	Machinery gene
MNR01_RS14760 (MNR01_14760)	-	3284971..3286491 (-)	1521	WP_241918514.1	hypothetical protein	-
MNR01_RS14765 (MNR01_14765)	-	3286973..3288919 (-)	1947	WP_241918515.1	hypothetical protein	-
MNR01_RS14770 (MNR01_14770)	pilE	3288993..3289421 (-)	429	WP_241918516.1	pilin	Machinery gene
MNR01_RS14775 (MNR01_14775)	pilR	3289836..3291284 (-)	1449	WP_241918517.1	sigma-54 dependent transcriptional regulator	Regulator
MNR01_RS14780 (MNR01_14780)	-	3291416..3293038 (-)	1623	WP_241918518.1	ATP-binding protein	-
MNR01_RS14785 (MNR01_14785)	sucC	3293271..3294437 (+)	1167	WP_241918519.1	ADP-forming succinate--CoA ligase subunit beta	-
MNR01_RS14790 (MNR01_14790)	sucD	3294450..3295325 (+)	876	WP_241918520.1	succinate--CoA ligase subunit alpha	-
MNR01_RS14795 (MNR01_14795)	-	3295451..3297388 (+)	1938	WP_256451847.1	acyltransferase family protein	-

Sequence

Protein

• Length: 574 a.a.
• Molecular weight: 62242.62 Da
• Isoelectric Point: 5.7937

>NTDB_id=663831 MNR01_RS14725 WP_241918507.1 3277852..3279576(-) (pilB) [Lysobacter sp. S4-A87]
MSTVATANLVGITGIARRLVLDGALDDAVARQALATASSLKQPIAGYLAEKRLVTAAQLAAANSIEFGVPILDATAFDPA
QSAIKVVSEALVRKHSVLPLFKRGNRLFIGIADPTNNQALEEIKFQTNLAIEPILVDEDRIKRCIETWLEASDALADSVG
DQDGLESLDVTAGDADLANETGVDVKTDDTPVVKFVNKVLVDAIRKGASDIHFEPYETEYRVRLRIDGLLKQVAKVPPSL
QARIAARVKVMAQLDIAEKRVPQDGRIKLNISKTKQIDFRVSTLPTLFGEKVVMRILDGSAAKLGIDKLGYEDDQRALYE
QALAKPYGMVLVTGPTGSGKTVSLYTGLNILNDEQRNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVI
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPFNITSSVTLVIAQRLARRLHDCKREVHLPEHAL
LAEGFKPEEIADGIKIYEAVGCQDCTEGYKGRTGIYQVMPMSDEIQAIVLAGGNAMQIADAALRSGINDLRQSALVKVKN
GVTSLAEINRVTKD

Nucleotide

• Length: 1725 bp

>NTDB_id=663831 MNR01_RS14725 WP_241918507.1 3277852..3279576(-) (pilB) [Lysobacter sp. S4-A87]
ATGAGCACCGTTGCCACTGCCAATCTGGTGGGGATCACCGGCATTGCCCGGCGCCTTGTGCTGGACGGGGCACTGGACGA
TGCAGTCGCCCGCCAGGCCCTGGCGACAGCCAGCAGCCTGAAGCAGCCGATCGCCGGTTACCTCGCGGAGAAGCGACTGG
TGACGGCCGCCCAGCTCGCTGCCGCCAATTCGATCGAATTCGGCGTGCCGATCCTGGATGCCACTGCATTCGACCCGGCG
CAGTCGGCCATAAAGGTGGTCAGTGAGGCGCTGGTCCGCAAGCATTCGGTGCTGCCGCTGTTCAAGCGCGGCAACCGCCT
CTTCATCGGAATCGCCGACCCGACCAACAACCAGGCGCTGGAAGAGATCAAGTTCCAGACCAACCTGGCGATCGAGCCGA
TCCTGGTCGACGAAGACCGCATCAAACGCTGTATCGAAACGTGGCTGGAAGCGAGCGACGCCCTGGCCGACAGCGTCGGT
GACCAGGATGGCCTGGAGTCGCTGGACGTCACGGCGGGCGATGCCGACCTGGCCAACGAAACTGGCGTCGACGTCAAGAC
CGACGACACACCGGTAGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCCATCCGCAAGGGCGCCTCCGACATCCATTTCG
AGCCCTACGAAACCGAATACCGCGTGCGCCTGCGCATTGACGGCCTGCTCAAGCAGGTCGCCAAGGTGCCGCCCAGCCTG
CAGGCCCGCATCGCCGCCCGCGTCAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGCCGCAT
CAAGCTCAACATCTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAAAAAGTGGTGA
TGCGTATCCTCGACGGCAGCGCCGCCAAGCTGGGCATCGACAAGCTCGGCTACGAAGACGACCAGCGCGCCCTCTATGAG
CAGGCGCTTGCCAAGCCCTACGGCATGGTGCTGGTCACCGGTCCGACCGGCTCGGGCAAGACCGTGTCGCTGTACACCGG
CCTGAACATCCTCAACGACGAGCAGCGCAATATCTCCACGGTCGAGGACCCGGTCGAAATCCGCGTGCCGGGCATCAATC
AGGTGCAGATGAACGTCAAGCGCGGCATGACCTTCGCCGCTGCGCTGCGAAGCTTCCTGCGCCAGGACCCGGACGTGATC
ATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCCATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTCCAC
CCTGCACACCAACGACGCGCCGCAGACCATCGCCCGCCTCATGAACATGGGCGTGGCGCCGTTCAACATCACCTCCTCGG
TCACCCTCGTGATCGCCCAGCGACTGGCGCGCCGTCTGCACGACTGCAAGCGCGAAGTGCATCTGCCCGAGCATGCATTG
CTGGCCGAAGGCTTCAAGCCGGAAGAAATTGCGGACGGGATCAAGATCTACGAAGCCGTCGGCTGCCAGGATTGCACCGA
AGGCTACAAGGGCCGTACCGGCATCTACCAGGTAATGCCGATGAGCGACGAGATCCAGGCGATCGTGCTCGCCGGCGGAA
ATGCAATGCAGATTGCGGACGCTGCCCTGCGCTCCGGCATCAACGACTTGCGTCAATCCGCACTTGTGAAAGTGAAGAAT
GGCGTTACCAGCCTCGCCGAAATCAACCGTGTAACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (48-148)

• Type II/IV secretion system protein (T2SSE) (198-465)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.477	98.606	0.547
pilB	Acinetobacter baumannii D1279779	54.225	98.955	0.537
pilB	Legionella pneumophila strain ERS1305867	52.205	98.78	0.516
pilB	Vibrio cholerae strain A1552	50.088	99.477	0.498
pilF	Neisseria gonorrhoeae MS11	49.811	91.986	0.458
pilB	Vibrio campbellii strain DS40M4	48.066	94.599	0.455
pilB	Vibrio parahaemolyticus RIMD 2210633	47.866	93.902	0.449
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.483	94.425	0.373