Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   LIW09_RS01705 Genome accession   NZ_CP093311
Coordinates   374898..376622 (-) Length   574 a.a.
NCBI ID   WP_256646260.1    Uniprot ID   -
Organism   Thermomonas paludicola strain IMCC34681     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 369898..381622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LIW09_RS01685 - 371631..372008 (+) 378 Protein_332 NUDIX domain-containing protein -
  LIW09_RS01690 coaE 372037..372675 (-) 639 WP_256646257.1 dephospho-CoA kinase -
  LIW09_RS01695 - 372736..373599 (-) 864 WP_256646258.1 A24 family peptidase -
  LIW09_RS01700 pilC 373618..374886 (-) 1269 WP_256646259.1 type II secretion system F family protein Machinery gene
  LIW09_RS01705 pilB 374898..376622 (-) 1725 WP_256646260.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LIW09_RS01710 - 376928..377194 (+) 267 WP_256646261.1 helix-turn-helix transcriptional regulator -
  LIW09_RS01715 - 377187..378416 (+) 1230 WP_256646262.1 type II toxin-antitoxin system HipA family toxin -
  LIW09_RS01720 - 378476..379336 (+) 861 WP_256646263.1 glycosyltransferase family 2 protein -
  LIW09_RS01725 - 379898..380611 (+) 714 WP_256646264.1 glycosyltransferase -
  LIW09_RS01730 - 380620..381369 (+) 750 WP_256646265.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62490.80 Da        Isoelectric Point: 6.3062

>NTDB_id=663460 LIW09_RS01705 WP_256646260.1 374898..376622(-) (pilB) [Thermomonas paludicola strain IMCC34681]
MNTATTPNLMGITGIARRLVMDGAMDEAVARKAMDGATAEKMPLATYLADRRLASPAALAAANSIEFGMPLVDALALDPA
QSAIALVKEDLLRKHMVLPLFKRGGRLFVGTSDPTNNRALEELKFHTNLAVEPILVDDDTIRRSLDSWLEAADAMADDLA
DSDGLEGLEVSGGDEDLSADTGVDSKGEDTPVVKFVNKALVDAIRRGASDIHFEPYENDYRVRYRIDGILKTAKRVPVKL
HARIAARLKVMSQLDIAEKRVPQDGRIKLNLSKGKQIDFRVSTLPTLFGEKVVLRILDGSAAKMGIEKLGYEPDQQKLFL
DAIHKPYGMVLVTGPTGSGKTVSLYTGLGILNDETRNISTVEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDPDVV
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVSLVIAQRLVRRLHDCKRAAHLPEHAL
LAEGFTADEVHAGITVYEAVGCQDCTEGYKGRAGIYQVMPMTDEIQQMVLAGGNVQQITEAALRSGVRDLRRSALDKVKQ
GITSLVEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=663460 LIW09_RS01705 WP_256646260.1 374898..376622(-) (pilB) [Thermomonas paludicola strain IMCC34681]
ATGAATACCGCAACCACCCCCAACCTGATGGGCATCACCGGCATCGCCCGACGTTTGGTGATGGATGGCGCCATGGACGA
AGCCGTGGCGCGCAAGGCGATGGATGGCGCCACGGCCGAAAAGATGCCGCTGGCCACGTACCTGGCCGACCGCCGGCTGG
CGTCCCCCGCGGCGCTGGCGGCGGCCAATTCCATCGAATTCGGGATGCCGCTGGTGGACGCGTTGGCGCTTGATCCCGCG
CAGTCGGCGATTGCGTTGGTCAAGGAAGACCTGCTGCGCAAGCATATGGTGTTGCCGCTGTTCAAGCGCGGCGGGCGGCT
GTTCGTGGGCACTTCCGACCCCACCAACAACCGGGCGCTGGAAGAGCTGAAATTCCACACCAACCTGGCGGTTGAGCCCA
TTTTGGTGGACGACGACACCATCAGGCGCAGCCTGGATTCCTGGCTGGAAGCGGCCGATGCCATGGCCGACGACTTGGCA
GACTCGGATGGTCTGGAGGGCCTGGAGGTCAGCGGCGGCGACGAAGATCTGTCTGCGGACACCGGTGTGGACTCCAAGGG
CGAGGACACGCCGGTCGTCAAGTTCGTCAACAAGGCGCTGGTGGATGCCATTCGTCGCGGCGCGTCGGACATCCATTTCG
AGCCCTACGAAAACGATTACCGGGTGCGTTACCGCATCGACGGCATCCTGAAAACGGCGAAAAGAGTGCCGGTGAAGCTG
CATGCGCGCATCGCCGCGCGCCTGAAGGTGATGTCGCAATTGGACATCGCGGAAAAGCGCGTGCCGCAGGACGGTCGCAT
CAAGCTCAACCTGAGCAAGGGCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCCACCTTGTTTGGCGAAAAGGTCGTGC
TGCGCATCCTGGACGGCAGCGCGGCCAAGATGGGCATCGAAAAGCTGGGCTACGAGCCCGATCAGCAGAAGCTGTTTCTG
GATGCGATCCACAAGCCCTACGGGATGGTGCTGGTCACCGGGCCCACCGGCTCGGGCAAGACCGTGAGCCTGTATACCGG
GCTGGGCATCCTCAACGACGAAACCCGCAACATCAGCACGGTCGAGGACCCGGTGGAAATCCGCCTGCCGGGCGTCAACC
AGGTCCAGCAAAACGTCAAGCGGGGAATGACGTTTGCTGCCGCGCTGCGCAGCTTCCTGCGTCAGGATCCGGACGTGGTG
ATGGTGGGCGAAATCCGCGATCTGGAAACTGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCATATGGTGTTGTCCAC
CCTGCACACCAACGACGCGCCGCAGACCATCGCCCGCCTGATGAACATGGGCATTGCGCCCTACAACATCACCAGTTCGG
TCAGCCTGGTCATCGCCCAGCGACTGGTGCGGCGCCTGCACGACTGCAAGCGCGCAGCGCACTTGCCCGAACACGCGCTG
CTGGCCGAGGGCTTCACCGCCGACGAGGTTCATGCGGGCATCACCGTGTATGAAGCGGTGGGCTGCCAGGACTGCACCGA
GGGCTACAAGGGTCGTGCCGGCATTTACCAGGTGATGCCGATGACCGATGAAATCCAGCAAATGGTGCTGGCAGGTGGTA
ATGTCCAGCAAATTACCGAGGCTGCCCTGCGCAGTGGCGTCCGCGATTTGCGGCGCTCCGCGCTGGACAAGGTGAAACAG
GGGATCACCAGCCTCGTCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(198-465)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

53.819

100

0.54
  pilB Acinetobacter baumannii D1279779

53.846

99.652

0.537
  pilB Legionella pneumophila strain ERS1305867

51.585

98.955

0.51
  pilF Neisseria gonorrhoeae MS11

48.319

98.432

0.476
  pilB Vibrio cholerae strain A1552

51.163

89.895

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

47.706

94.948

0.453
  pilB Vibrio campbellii strain DS40M4

49.808

90.592

0.451
  pilB/pilB1 Synechocystis sp. PCC 6803

39.429

91.463

0.361
  pilF Thermus thermophilus HB27

39.655

90.941

0.361