Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AS19_RS03180 Genome accession   NZ_AP014613
Coordinates   674803..676509 (-) Length   568 a.a.
NCBI ID   WP_046961687.1    Uniprot ID   A0AAD1B043
Organism   Alcanivorax sp. NBRC 101098     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 669803..681509
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AS19_RS03155 (AS19_06260) - 670739..671677 (+) 939 WP_046961686.1 Nudix family hydrolase -
  AS19_RS03160 (AS19_06270) - 671791..671991 (-) 201 WP_011587895.1 DNA gyrase inhibitor YacG -
  AS19_RS03165 (AS19_06280) coaE 672013..672633 (-) 621 WP_011587896.1 dephospho-CoA kinase -
  AS19_RS03170 (AS19_06290) pilD 672696..673571 (-) 876 WP_011587897.1 A24 family peptidase Machinery gene
  AS19_RS03175 (AS19_06300) pilC 673574..674800 (-) 1227 WP_035460653.1 type II secretion system F family protein Machinery gene
  AS19_RS03180 (AS19_06310) pilB 674803..676509 (-) 1707 WP_046961687.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AS19_RS14200 (AS19_06320) comP 677044..677490 (+) 447 WP_046962267.1 pilin Machinery gene
  AS19_RS03190 (AS19_06330) - 677506..678873 (+) 1368 WP_046961688.1 O-antigen ligase -
  AS19_RS03195 (AS19_06340) nadC 678929..679771 (-) 843 WP_046961689.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AS19_RS03200 (AS19_06350) - 679887..681224 (-) 1338 WP_011587903.1 sodium:alanine symporter family protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62765.48 Da        Isoelectric Point: 5.0177

>NTDB_id=66206 AS19_RS03180 WP_046961687.1 674803..676509(-) (pilB) [Alcanivorax sp. NBRC 101098]
MTSQTTSFSGLPHRLIQQGLLDAESIQLALQQARQEKTSLIKQIVASGKLTARQVASAVAEEFGDPIFDLNSFQRDSLVK
DLVDPKLINKHNVLPLYRRGSRLFVAVSDPTNLPALEEIRFNTGLNVETVIVEEDKLSQWIEALESETEDDAFSDLDAGL
ENLDISSNEETEDETQSGADDAPIVRFVNKLLLDAIKGGASDLHFEPYEKTYRVRFRQDGILQTVSKPPINIANKLSARL
KVMSRLDISERRVPQDGRIKLKISKTRAIDFRVNTCPTLFGEKIVLRILDPESAKMGIEALGYENDQKQMYLDALHKPQG
MILVTGPTGSGKTVSLYTGVNILNTPELNISTAEDPVEINLEGVNQVNVNPKQGMNFSSALRAFLRQDPDVILVGEIRDL
ETAEIAVKAAQTGHLVLSTLHTNSAPETLTRLRNMGVPSFNIATSVLLIIAQRLARRLCENCKTPVDIPKQSLLEMGFTE
DDIANEFTVYGPVGCDKCKNGYKGRVGIYEVVKITDGIARIIMEEGNSIEIADQCRKEGFNNLHRSGLVKAMQGVTSLEE
INRVTSGH

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=66206 AS19_RS03180 WP_046961687.1 674803..676509(-) (pilB) [Alcanivorax sp. NBRC 101098]
ATGACTAGCCAAACCACCTCATTTAGCGGCTTACCGCATCGATTAATACAGCAGGGCCTGCTAGACGCAGAGAGCATACA
GCTAGCCCTGCAACAGGCTCGCCAAGAAAAAACCAGCCTTATTAAACAGATCGTTGCTAGTGGGAAACTTACGGCTAGGC
AGGTAGCAAGCGCCGTTGCGGAAGAATTTGGCGACCCTATTTTTGACCTTAACAGCTTCCAGCGCGATAGCTTAGTAAAG
GACCTTGTCGACCCTAAGCTGATCAATAAACACAACGTACTCCCCCTTTACCGGCGCGGAAGCCGCCTCTTTGTTGCGGT
ATCCGATCCTACTAATTTACCTGCGCTAGAAGAAATCCGTTTTAATACTGGCCTTAATGTAGAAACGGTGATTGTTGAGG
AAGACAAGCTAAGCCAATGGATCGAAGCGTTGGAGTCCGAGACCGAAGATGATGCCTTTAGCGACCTGGATGCCGGACTA
GAAAACCTGGACATTAGTTCGAATGAAGAAACAGAAGATGAAACTCAATCTGGTGCCGATGATGCACCAATTGTCCGCTT
CGTGAATAAACTTCTTCTTGATGCCATTAAGGGCGGAGCATCAGATTTGCATTTCGAGCCCTATGAGAAAACCTATCGGG
TGCGCTTCCGTCAAGATGGCATCCTGCAAACCGTATCGAAACCACCTATCAATATCGCCAACAAACTTTCTGCGCGCCTA
AAGGTCATGTCTCGGCTGGATATTTCAGAACGTCGAGTCCCTCAGGATGGCCGGATAAAGTTGAAAATTTCCAAGACACG
GGCCATCGATTTCCGGGTGAACACCTGCCCCACCTTATTTGGTGAAAAAATTGTATTGCGTATTCTGGACCCGGAATCCG
CAAAGATGGGCATCGAGGCTTTGGGTTATGAAAATGATCAGAAACAGATGTACCTGGATGCCCTGCATAAACCACAAGGC
ATGATACTCGTCACTGGCCCCACCGGCTCAGGAAAGACCGTTTCACTATACACCGGAGTAAACATTCTCAATACGCCAGA
ACTCAATATTTCCACCGCCGAAGACCCGGTTGAAATCAACTTGGAGGGGGTTAACCAGGTTAATGTGAACCCGAAACAAG
GGATGAATTTCTCATCAGCACTACGTGCCTTCCTACGCCAGGATCCCGACGTCATTCTGGTGGGAGAAATTCGTGACTTA
GAAACAGCAGAAATAGCTGTGAAAGCGGCCCAGACAGGTCACCTGGTACTTTCCACCCTCCACACTAACAGCGCACCGGA
AACTCTAACTCGTCTTCGCAACATGGGCGTTCCCTCCTTCAACATTGCCACCTCGGTATTATTAATTATTGCCCAACGTT
TAGCCCGGCGCCTGTGTGAAAACTGCAAAACTCCAGTGGATATTCCTAAACAATCCTTGCTTGAGATGGGTTTCACGGAA
GACGATATCGCCAACGAGTTTACTGTGTACGGACCGGTAGGGTGCGACAAATGCAAGAACGGCTACAAAGGGCGCGTGGG
GATTTATGAAGTCGTTAAAATCACAGACGGCATCGCGCGCATTATTATGGAAGAGGGTAACTCTATCGAAATTGCCGACC
AGTGCCGCAAGGAGGGCTTTAATAACCTTCACCGCTCCGGATTGGTAAAAGCAATGCAAGGGGTTACCAGCCTAGAAGAA
ATTAACCGTGTCACAAGCGGACATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

62.632

100

0.629
  pilB Acinetobacter baylyi ADP1

60.664

100

0.611
  pilB Legionella pneumophila strain ERS1305867

54.895

100

0.553
  pilB Vibrio parahaemolyticus RIMD 2210633

50.352

100

0.504
  pilB Vibrio campbellii strain DS40M4

50.447

98.415

0.496
  pilB Vibrio cholerae strain A1552

49.643

98.592

0.489
  pilF Neisseria gonorrhoeae MS11

48.387

98.239

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

37.398

100

0.405
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.545

96.831

0.373
  pilF Thermus thermophilus HB27

37.838

97.711

0.37

Multiple sequence alignment