Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MML47_RS06735 Genome accession   NZ_CP092971
Coordinates   1469349..1470479 (+) Length   376 a.a.
NCBI ID   WP_053503119.1    Uniprot ID   -
Organism   Xanthomonas oryzae strain NJ01     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1464349..1475479
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MML47_RS06710 (MML47_06710) - 1464525..1465166 (-) 642 WP_053503116.1 M23 family metallopeptidase -
  MML47_RS06715 (MML47_06715) - 1465272..1465697 (-) 426 WP_011258216.1 HU family DNA-binding protein -
  MML47_RS06720 (MML47_06720) proC 1466254..1467114 (-) 861 WP_053503117.1 pyrroline-5-carboxylate reductase -
  MML47_RS06725 (MML47_06725) - 1467158..1467850 (-) 693 WP_053503118.1 YggS family pyridoxal phosphate-dependent enzyme -
  MML47_RS06730 (MML47_06730) pilT 1468198..1469235 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  MML47_RS06735 (MML47_06735) pilU 1469349..1470479 (+) 1131 WP_053503119.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MML47_RS06740 (MML47_06740) - 1470751..1471425 (+) 675 WP_024711780.1 YitT family protein -
  MML47_RS06750 (MML47_06750) - 1471634..1472432 (+) 799 WP_241442834.1 IS5 family transposase -
  MML47_RS06755 (MML47_06755) - 1472504..1473337 (-) 834 WP_053503121.1 DUF72 domain-containing protein -
  MML47_RS06760 (MML47_06760) - 1473374..1473946 (-) 573 WP_053503122.1 DNA-3-methyladenine glycosylase I -
  MML47_RS06765 (MML47_06765) - 1473943..1475153 (-) 1211 Protein_1293 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41970.20 Da        Isoelectric Point: 6.8287

>NTDB_id=661186 MML47_RS06735 WP_053503119.1 1469349..1470479(+) (pilU) [Xanthomonas oryzae strain NJ01]
MSTIDFTSFLKLMAHQKASDLFITSGMPPAIKVNGKISPITQTPLTAQQSRDLVLNVMTPSQREEFEKTHECNFAIGVSG
VGRFRVSCFYQRNQVGMVLRRIETRIPTVDELSLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGHRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWESALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLKGVVAQQLIPTPDGRSRRVAMEIMLGTPLVQDYIRDGEIHKLKEIMKESTNLGMRTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLSQGGDAKTLSQGMDGVEISEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=661186 MML47_RS06735 WP_053503119.1 1469349..1470479(+) (pilU) [Xanthomonas oryzae strain NJ01]
ATGAGCACCATCGACTTCACCTCCTTCCTCAAGCTGATGGCGCATCAGAAGGCGTCGGACCTGTTCATCACCTCGGGGAT
GCCACCGGCGATCAAGGTGAATGGCAAGATCAGCCCGATTACCCAGACGCCGCTGACTGCGCAGCAGAGCCGCGATCTGG
TGTTGAACGTGATGACGCCCTCGCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTTTCAGGG
GTGGGGCGTTTTCGTGTGAGCTGTTTCTACCAGCGTAATCAGGTCGGTATGGTGCTGCGCCGGATCGAAACGCGCATCCC
CACCGTGGACGAGCTGAGCCTGCCTCCGGTGATCAAGACGCTGGCGATGACCAAGCGCGGCATCATCATCTTCGTCGGCG
CCACCGGTACCGGTAAGTCGACCTCGCTGGCGGCGATGATCGGCCACCGCAACCAGAATTCCACCGGCCACATCATCACC
ATCGAAGACCCGATCGAATTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGATACCGACAG
CTGGGAAAGCGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGTACCCGCGAGGGCA
TGGACCACGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACGCTGCACGCCAACAACGCCAATCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAATCAGCTGCTGATGGACCTGTCGCTCAATCTCAAGGGCGTGGT
GGCGCAGCAGCTGATTCCGACGCCGGATGGCCGCAGCCGCCGCGTGGCGATGGAAATCATGTTGGGCACGCCGCTGGTGC
AGGACTACATCCGCGACGGCGAAATCCACAAGCTCAAGGAGATCATGAAGGAGTCCACCAACCTGGGCATGCGCACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCTGGCGAAATCAGCTACGAAGATGCATTGCGCTACGCCGACTCGCAGAACGA
AGTGCGCTTGCGCATCAAGCTCTCGCAGGGGGGCGATGCCAAGACCTTGTCGCAGGGCATGGATGGTGTGGAGATTTCCG
AAGTTCGATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(108-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.986

94.947

0.617
  pilU Vibrio cholerae strain A1552

52.809

94.681

0.5
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.017

94.947

0.399
  pilT Acinetobacter nosocomialis M2

39.535

91.489

0.362
  pilT Acinetobacter baumannii D1279779

39.535

91.489

0.362
  pilT Acinetobacter baumannii strain A118

39.535

91.489

0.362