Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MME54_RS01645 Genome accession   NZ_CP092918
Coordinates   361168..363273 (+) Length   701 a.a.
NCBI ID   WP_054987983.1    Uniprot ID   -
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 356168..368273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS01625 (MME54_01625) pilM 358331..359395 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  MME54_RS01630 (MME54_01630) - 359395..359976 (+) 582 WP_054987980.1 PilN domain-containing protein -
  MME54_RS01635 (MME54_01635) pilO 359973..360596 (+) 624 WP_054987981.1 type 4a pilus biogenesis protein PilO -
  MME54_RS01640 (MME54_01640) pilP 360593..361120 (+) 528 WP_054987982.1 type 4a pilus biogenesis lipoprotein PilP -
  MME54_RS01645 (MME54_01645) pilQ 361168..363273 (+) 2106 WP_054987983.1 type IV pilus secretin PilQ Machinery gene
  MME54_RS01650 (MME54_01650) aroK 363278..363796 (+) 519 WP_054987984.1 shikimate kinase AroK -
  MME54_RS01655 (MME54_01655) aroB 363912..365015 (+) 1104 WP_054987985.1 3-dehydroquinate synthase -
  MME54_RS01660 (MME54_01660) - 365026..366651 (+) 1626 WP_054987986.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 701 a.a.        Molecular weight: 75553.89 Da        Isoelectric Point: 5.1826

>NTDB_id=661115 MME54_RS01645 WP_054987983.1 361168..363273(+) (pilQ) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MTRIFSIIGVSLCIAMLSPVLQAANLKTLDVAALPGDRIELKLAFDAPVPAPRGYTTAQPARIALDLPGVTSQLATKSRD
LGTGNARSVVVVQAQDRTRVIINLTTLSPYSSRVDGNNLYVVIGQGAAAAQASQPTTAMGAQRVAVPPPTPASARPFIPA
GARAIRNVDFQRGELGEGNVVIDLSNPSIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVSASASGDKASI
LIEPSGAFDYSAFQTENKLTISVRPLTNEDLDRRAADKPVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGI
TLRLQNVPWDQALDLVLKTKGLDKRKVGSVLLVAPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQS
VTSAESKTDERGSITVDDRTNNIIAYQTQERLDELRRIVSQLDIPVRQVMIEARIVEANVDYNKQLGVRWGGSTNTSGNR
KWTTYGLDNNGDEAGNTGSNVTSNVPFVDLGASGATSGIGLGFVTNNTLLDLELSAMEKTGNGEIVSQPKVVTSDKETAK
ILKGTEIPYQESSSSGATTVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNAVLGVPPIKKNEVNAKVLISDGET
IVIGGVFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSESKSELLVFLTPRIMNNQAISVSR

Nucleotide


Download         Length: 2106 bp        

>NTDB_id=661115 MME54_RS01645 WP_054987983.1 361168..363273(+) (pilQ) [Pseudomonas syringae pv. helianthi strain LMG 5067]
ATGACCCGGATTTTCTCGATTATCGGCGTGTCGTTATGCATCGCGATGCTGTCGCCGGTTCTCCAGGCTGCCAACCTCAA
GACCCTGGATGTCGCCGCCCTGCCGGGTGATCGCATCGAACTCAAGCTGGCATTCGACGCGCCGGTGCCTGCGCCGCGCG
GCTATACCACGGCGCAACCGGCGCGTATCGCGCTGGACCTGCCGGGTGTGACCAGTCAGCTGGCGACCAAAAGCCGTGAC
CTGGGCACCGGCAATGCACGCAGCGTGGTCGTGGTGCAGGCCCAGGACCGCACGCGGGTCATCATCAACCTGACCACGCT
CTCGCCTTACAGCTCCCGTGTCGACGGCAACAACCTGTACGTGGTTATCGGCCAGGGTGCCGCCGCTGCGCAGGCTTCCC
AGCCAACCACCGCGATGGGCGCGCAGCGCGTAGCGGTGCCGCCACCGACGCCGGCATCTGCCAGACCGTTTATTCCCGCA
GGTGCCAGAGCCATCAGAAATGTCGACTTCCAGCGCGGCGAGCTGGGTGAAGGCAATGTGGTCATAGACCTGAGCAATCC
CAGCATCGCTCCGGACATTCAGGAGCAGGGCGGCAAGATTCGTGTGGACTTCGCCAAAACCCAACTGCCCGAGCCGCTGC
GCGTCCGTCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTTGTCAGTGCCTCGGCTTCGGGCGACAAGGCCAGTATT
CTGATCGAACCTTCGGGTGCCTTCGATTACTCGGCGTTTCAGACCGAAAACAAGCTGACCATCAGTGTGCGTCCGTTGAC
CAACGAAGACCTGGACCGCCGCGCCGCCGACAAGCCGGTGTACACCGGTGAAAAGCTGTCACTGAACTTCCAGGACATCG
ACGTGCGTTCGGTACTGCAGTTGATTGCCGACTTCACTAACCTCAATCTGGTTGCCAGCGATACTGTTCAGGGCGGCATC
ACCCTGCGTCTGCAGAATGTGCCGTGGGATCAGGCACTGGACCTGGTGCTGAAAACCAAAGGGCTGGACAAGCGCAAGGT
CGGCAGCGTTCTGCTGGTCGCGCCTGCCGATGAAATTGCCGCCCGTGAGCGTCAGGAGCTTGAGTCGCTCAAGCAGATCG
CCGAGCTGGCACCCCTGCGTCGTGAGCTGCTGCAGGTCAATTACGCCAAGGCTGCCGACATTGCCAAGCTTTTCCAGTCG
GTGACCAGTGCCGAGTCCAAGACCGACGAACGCGGCTCGATCACGGTAGACGACCGGACCAACAACATCATTGCCTATCA
GACCCAGGAGCGGCTCGACGAACTGCGCCGTATCGTGTCGCAACTGGACATTCCTGTGCGCCAGGTGATGATCGAGGCGC
GTATCGTCGAGGCCAATGTCGATTACAACAAGCAGCTGGGTGTTCGCTGGGGCGGCTCGACCAACACCAGCGGCAATAGA
AAGTGGACAACCTACGGTCTGGACAATAACGGCGATGAGGCTGGCAACACCGGCAGCAACGTGACGTCCAACGTGCCGTT
CGTCGACCTCGGCGCCTCTGGCGCGACCTCCGGGATTGGTCTTGGCTTCGTGACCAACAACACGTTGCTGGACCTGGAAC
TGAGCGCTATGGAGAAAACCGGCAACGGTGAAATCGTCTCGCAGCCCAAGGTCGTTACCTCCGACAAGGAGACCGCCAAG
ATCCTCAAGGGCACGGAAATTCCGTATCAGGAATCCAGCTCCAGTGGCGCAACGACGGTGAGCTTCAAGGAGGCTTCACT
GTCGCTTGAAGTCACACCGCAGATCACTCCCGATAATCGCATCATCATGGAAGTGAAGGTCACCAAGGACGAGCCGGATT
ACCTGAATGCGGTGCTGGGTGTGCCGCCCATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGATTTCCGATGGCGAAACC
ATCGTCATCGGTGGTGTGTTCTCGAATACGCAAAGTAAAGTTGTCGATAAAGTGCCATTTCTTGGCGATGTACCGTATCT
TGGCCGGCTTTTCAGGCGTGATGTGGTATCCGAGTCCAAATCCGAGCTGTTGGTATTTCTGACTCCGCGTATCATGAACA
ACCAGGCGATTTCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(309-355)

Bacterial type II and III secretion system protein (Secretin)

(536-692)

Bacterial type II/III secretion system short domain (Secretin_N)

(383-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.614

100

0.749
  comQ Acinetobacter baylyi ADP1

41.008

100

0.429
  pilQ Acinetobacter baumannii strain A118

38.255

100

0.407
  pilQ Acinetobacter baumannii D1279779

38.255

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.295

98.716

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.295

98.716

0.378