Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MME54_RS01625 Genome accession   NZ_CP092918
Coordinates   358331..359395 (+) Length   354 a.a.
NCBI ID   WP_002551652.1    Uniprot ID   A0A7Z6Y2Y4
Organism   Pseudomonas syringae pv. helianthi strain LMG 5067     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 353331..364395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MME54_RS01615 (MME54_01615) - 354296..355564 (+) 1269 WP_054987978.1 malic enzyme-like NAD(P)-binding protein -
  MME54_RS01620 (MME54_01620) - 355683..358121 (-) 2439 WP_371913042.1 penicillin-binding protein 1A -
  MME54_RS01625 (MME54_01625) pilM 358331..359395 (+) 1065 WP_002551652.1 pilus assembly protein PilM Machinery gene
  MME54_RS01630 (MME54_01630) - 359395..359976 (+) 582 WP_054987980.1 PilN domain-containing protein -
  MME54_RS01635 (MME54_01635) pilO 359973..360596 (+) 624 WP_054987981.1 type 4a pilus biogenesis protein PilO -
  MME54_RS01640 (MME54_01640) pilP 360593..361120 (+) 528 WP_054987982.1 type 4a pilus biogenesis lipoprotein PilP -
  MME54_RS01645 (MME54_01645) pilQ 361168..363273 (+) 2106 WP_054987983.1 type IV pilus secretin PilQ Machinery gene
  MME54_RS01650 (MME54_01650) aroK 363278..363796 (+) 519 WP_054987984.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38078.57 Da        Isoelectric Point: 4.7008

>NTDB_id=661114 MME54_RS01625 WP_002551652.1 358331..359395(+) (pilM) [Pseudomonas syringae pv. helianthi strain LMG 5067]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLAKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKDALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=661114 MME54_RS01625 WP_002551652.1 358331..359395(+) (pilM) [Pseudomonas syringae pv. helianthi strain LMG 5067]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTCGAGCCATTGCCGGCCAACGCCGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTTGGCCAGGCGCTGTCTCGCGTGCTGGCCAAGGCCAAGACCAGCGTCAAGATTGTGGCGGTC
GCCGTAGCCGGTTCTGCCGTGATCACCAAGACAATCGAGATGGACGCCGGTCTTTCCGATGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCTTACCCGCTCGAAGAGGTCGCCATCGATTTCGAAGTGCAGGGCTATTCGGTGC
GCAATCCCGAACGCGTCGAAGTGTTGCTGGCTGCCTGCCGCAAGGAGAACGTCGAGGTGCGTGAGGCGGCACTGGCGCTG
GCCGGGCTGACCGCGCGGGTGGTGGATGTCGAGGCCTATGCCCTTGAGCGTTCGTTCGGTCTGCTGGCTGCGCAACTGGG
CAACGGTCACGACGAGCTTACCGTTGCCGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACCATGGCC
GGATCATTTATACCCGCGAGCAGTTGTTCGGTGGTCGCCAACTGACGGACGAAATCCAGCGTCGCTACGGGCTTTCCATG
GAAGAAGCCGGGCTCGCCAAGAAGCAGGGCGGGCTGCCCGACGATTATGTGAGCGAGGTCCTGGACCCGTTCAAGGACGC
GCTGGTGCAGCAGGTTTCCCGTTCCCTGCAGTTCTTCTTTGCGGCCGGTCAATACAACTCTGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAACACTTGATTCAGCGACGTATCGGCACCCCCACCATGGTTGCCAACCCG
TTTGCTGACATGGCCTTGAGCTCCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCACCCGCCTTGATGATCGCGTGCGG
TCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z6Y2Y4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.761

100

0.469