Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MKW86_RS02070 Genome accession   NZ_CP092906
Coordinates   448189..449895 (-) Length   568 a.a.
NCBI ID   WP_073351950.1    Uniprot ID   -
Organism   Aeromonas hydrophila strain LP0103     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 443189..454895
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MKW86_RS02040 (MKW86_02040) mutT 443866..444279 (+) 414 WP_174892455.1 8-oxo-dGTP diphosphatase MutT -
  MKW86_RS02045 (MKW86_02045) yacG 444340..444534 (-) 195 WP_005305661.1 DNA gyrase inhibitor YacG -
  MKW86_RS02050 (MKW86_02050) zapD 444544..445266 (-) 723 WP_043160635.1 cell division protein ZapD -
  MKW86_RS02055 (MKW86_02055) coaE 445304..445918 (-) 615 WP_073351951.1 dephospho-CoA kinase -
  MKW86_RS02060 (MKW86_02060) pilD 445937..446809 (-) 873 WP_065475375.1 A24 family peptidase Machinery gene
  MKW86_RS02065 (MKW86_02065) pilC 446832..448073 (-) 1242 WP_011707570.1 type II secretion system F family protein Machinery gene
  MKW86_RS02070 (MKW86_02070) pilB 448189..449895 (-) 1707 WP_073351950.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  MKW86_RS02075 (MKW86_02075) tapA 449899..450342 (-) 444 WP_073352603.1 type IVa pilus major pilin TapA -
  MKW86_RS02080 (MKW86_02080) nadC 450665..451522 (-) 858 WP_130631564.1 carboxylating nicotinate-nucleotide diphosphorylase -
  MKW86_RS02085 (MKW86_02085) - 451528..451998 (-) 471 WP_024944772.1 retropepsin-like aspartic protease -
  MKW86_RS02090 (MKW86_02090) ampD 452146..452718 (+) 573 WP_241417178.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  MKW86_RS02095 (MKW86_02095) pdhR 452994..453758 (+) 765 WP_011707564.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62511.90 Da        Isoelectric Point: 5.4363

>NTDB_id=661023 MKW86_RS02070 WP_073351950.1 448189..449895(-) (pilB) [Aeromonas hydrophila strain LP0103]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTTAIGKLMESDQDALGMEDIDESEI
SELEVSDEGSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEVATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDERQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=661023 MKW86_RS02070 WP_073351950.1 448189..449895(-) (pilB) [Aeromonas hydrophila strain LP0103]
ATGACCTCTAGCCCCAATAGCGGCCTGGCATTAAGCCTGGCCGCTTCTTCCCTGCTCAGTGAGTCCGACTCACAGCGCTA
TCTGAGTCAAGCCAAGGCCCAGCGCAAGCCCTTTGTGACCTTTCTGATCGAAAACGAAATCCTCGACAGCAAGGCACTGG
CCGACTTCTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAATAC
CTCAATCAGAAGCTGATTGAAAAACATCACGTCCTGCCCATCTACACCCAGGGGCACACCCTCTATATCGCCATGTCGGA
TCCGACCAATGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGTTGCATACCGAGGCGCTGTTGGTCGAGGAGAACA
AACTCACCACTGCCATCGGCAAGCTGATGGAGAGCGATCAGGATGCCCTCGGCATGGAGGACATCGACGAATCCGAGATC
TCCGAACTGGAGGTCTCGGACGAGGGATCGCGGCTCGACGAGAGCGTCAATACGGCTGACGATGACGCCCCCATCGTCAA
ATACATCAACAAGATCATGATGGATGCCATCAAGCGTGGAGCCTCCGACCTTCACTTCGAGCCCTATGAGACCAAATACC
GCATCCGCTTTCGGATAGACGGGATCTTGCATGAGGTAGCAACTCCGCCAGTCAATCTGGCCAACCGCTTCTCGGCACGC
CTCAAGGTGATGGCACGGCTCGATATCGCCGAGCGGCGACTGCCCCAGGATGGTCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGCGAAAAGATCGTTATCCGTCTGCTCGACTCCTCCG
CCGCACGCCTCAATATCGAGCAACTCGGTTTCGACGAGCGCCAGAAGGCGCAATACCTGCGTGCCCTGTCGAAGCCTCAG
GGCATGATTCTGGTGACCGGCCCTACCGGTTCGGGCAAGACGGTTTCACTCTATACCGGTCTCAATATTCTCAATACCAC
CGAGGTCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACCTGCCCGGCGTCAATCAGGTGCAAGTCAATCCCAAGG
CAGGGCTGACCTTCGCCAGCGCCCTGCGTTCTTTCTTGCGCCAAGACCCCGATGTGGTCATGGTGGGTGAAATCCGGGAT
CTGGAGACCGCTGAAATTGCCATCAAGGCCGCCCAGACCGGCCACCTGGTGCTATCCACCCTACACACCAACTCGGCGGC
AGAAACGCTGACCCGGATGATGAACATGGGGGTGCCCGCCTTCAATATCGCCTCCTCGGTCACCCTGATCATGGCTCAGC
GGCTGGCGCGCAAACTGTGCGATCACTGCAAAGCCCCCGAAGTGGTACCCGAGGCTGAGCTGCTTGAGCTGGGTTTTACC
CAGCAGCAGCTGGCCGCTGGCTTGCGGCTGTTCAAACCGGTTGGCTGCAAAGAGTGCTCAGGTGGGTACAAGGGCAGGGT
CGGCATCTACGAAATCATGCTAATGTCTGAAAACATCGCAAAATTGATCATGCAGGGGGCCAACTCTCTGCAAATTGCCG
CCATCGCCCAGAAGGAGGGCATGCGCACCTTGCGTACCTCTGGCCTTGAGAAGGCTCGCCTGGGGGTGACCAGTCTGGCG
GAGATAAACCGGGTAACAACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.712

100

0.558
  pilB Acinetobacter baylyi ADP1

55.263

100

0.555
  pilB Legionella pneumophila strain ERS1305867

54.513

99.472

0.542
  pilB Vibrio cholerae strain A1552

54.159

99.472

0.539
  pilB Vibrio campbellii strain DS40M4

51.59

99.648

0.514
  pilB Vibrio parahaemolyticus RIMD 2210633

51.601

98.944

0.511
  pilF Neisseria gonorrhoeae MS11

46.631

99.296

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.355

85.211

0.361