Detailed information NTG0659971

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: MK801_RS05860
• Genome accession: NZ_CP092748
• Coordinates: 1140480..1141328 (-)
• Length: 282 a.a.
• NCBI ID: WP_057720839.1
• Uniprot ID: A0A443LFA4
• Organism: Lactococcus lactis strain 17M1
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1135480..1146328

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MK801_RS05845 (MK801_05840)	xerS	1135556..1136626 (+)	1071	WP_003131037.1	tyrosine recombinase XerS	-
MK801_RS05850 (MK801_05845)	trmFO	1136671..1138014 (-)	1344	WP_025017071.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
MK801_RS05855 (MK801_05850)	topA	1138086..1140218 (-)	2133	WP_023189256.1	type I DNA topoisomerase	-
MK801_RS05860 (MK801_05855)	dprA	1140480..1141328 (-)	849	WP_057720839.1	DNA-processing protein DprA	Machinery gene
MK801_RS05865 (MK801_05860)	-	1141474..1141854 (-)	381	WP_015426409.1	RidA family protein	-
MK801_RS05870 (MK801_05865)	budA	1141895..1142605 (-)	711	WP_057720838.1	acetolactate decarboxylase	-
MK801_RS05875 (MK801_05870)	ilvA	1142721..1143971 (-)	1251	WP_057720837.1	threonine ammonia-lyase IlvA	-
MK801_RS05880 (MK801_05875)	ilvC	1144027..1145049 (-)	1023	WP_128267760.1	ketol-acid reductoisomerase	-
MK801_RS05885 (MK801_05880)	ilvN	1145095..1145571 (-)	477	WP_010905839.1	acetolactate synthase small subunit	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 32046.12 Da
• Isoelectric Point: 9.3799

>NTDB_id=659971 MK801_RS05860 WP_057720839.1 1140480..1141328(-) (dprA) [Lactococcus lactis strain 17M1]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVIKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIGLLKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIADGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide

• Length: 849 bp

>NTDB_id=659971 MK801_RS05860 WP_057720839.1 1140480..1141328(-) (dprA) [Lactococcus lactis strain 17M1]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACGTAATTAAACTGAGAGAAGAGTATAAAATATACCCTTCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGGCTCAAGGAAATTTACAATCCACCTGTTCTATTATTTTATCAAGGAGATATAGGACTCTTAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAATATCTTGCTAAAAACCA
ACTAATTCTATCAGAATATTCAGTCGGAGAAAAACCACTTCGCTATCATTTTCCTGAACGCAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCAGATGGATTTTCAGATGGCTGTAATCATCTAATTCAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (76-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

3D structure

• AlphaFold DB: A0A443LFA4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	86.879	100	0.869
dprA	Streptococcus mutans UA159	60.072	98.582	0.592
dprA/cilB/dalA	Streptococcus mitis SK321	54.643	99.291	0.543
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae D39	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae R6	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.929	99.291	0.535
dprA	Legionella pneumophila strain ERS1305867	38.768	97.872	0.379
dprA	Haemophilus influenzae Rd KW20	38.889	95.745	0.372