Detailed information NTG0659948

Bioinformatically predicted

Overview

• Name: comGB
• Type: Machinery gene
• Locus tag: MK801_RS00780
• Genome accession: NZ_CP092748
• Coordinates: 163250..164323 (+)
• Length: 357 a.a.
• NCBI ID: WP_080619357.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain 17M1
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 158250..169323

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MK801_RS00770 (MK801_00770)	-	161807..162148 (+)	342	WP_240664866.1	hypothetical protein	-
MK801_RS00775 (MK801_00775)	comGA	162418..163356 (+)	939	Protein_135	competence type IV pilus ATPase ComGA	-
MK801_RS00780 (MK801_00780)	comGB	163250..164323 (+)	1074	WP_080619357.1	competence type IV pilus assembly protein ComGB	Machinery gene
MK801_RS00785 (MK801_00785)	comGC	164337..164720 (+)	384	WP_080651958.1	competence type IV pilus major pilin ComGC	Machinery gene
MK801_RS00790 (MK801_00790)	comGD	164695..165111 (+)	417	WP_240664867.1	competence type IV pilus minor pilin ComGD	Machinery gene
MK801_RS00795 (MK801_00795)	comGE	165083..165379 (+)	297	WP_015427164.1	competence type IV pilus minor pilin ComGE	Machinery gene
MK801_RS00800 (MK801_00800)	comGF	165342..165788 (+)	447	WP_057720549.1	competence type IV pilus minor pilin ComGF	Machinery gene
MK801_RS00805 (MK801_00805)	comGG	165836..166111 (+)	276	WP_240758564.1	competence protein ComGG	Machinery gene
MK801_RS00810 (MK801_00810)	-	166193..166630 (+)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
MK801_RS00815 (MK801_00815)	-	166627..167469 (+)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
MK801_RS00820 (MK801_00820)	-	167646..168383 (+)	738	WP_015427159.1	metal ABC transporter ATP-binding protein	-
MK801_RS00825 (MK801_00825)	-	168376..169185 (+)	810	WP_014570791.1	metal ABC transporter permease	-

Sequence

Protein

• Length: 357 a.a.
• Molecular weight: 40358.52 Da
• Isoelectric Point: 10.3132

>NTDB_id=659948 MK801_RS00780 WP_080619357.1 163250..164323(+) (comGB) [Lactococcus lactis strain 17M1]
MSLGHQKIGIKKLISYLQMDISHLLKPRGKKLRLIKQAKLIQLMGNLLNSGFNLGEVINFLSLSKLVEKEFTLKMKEGLA
SGQALSELLESLSFSKNVVTQLALVEVHGNLSGTMQLVELHLKKQLKVKNKLVEVATYPILLLIFLVGIMWGLKNYLLPQ
LNSGSNFATLLINHLPLVFFSFGAFIFLLTALSVTLFKRKSAIMNFTFLVKLPLVHSFIRLYLTAYFAREWGNLIAQGVE
LRQIINLMKKQKSRIFSEVGKNLDLELNAGRSFEQAVSKLALFLPELSLMIEYGAIKDKLGLELSLYADECWEQFFTKID
RLMQLIQPLVFIFVALMIILLYAAMLLPIYSNMGSGI

Nucleotide

• Length: 1074 bp

>NTDB_id=659948 MK801_RS00780 WP_080619357.1 163250..164323(+) (comGB) [Lactococcus lactis strain 17M1]
TTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTACTTACAAATGGATATCTCACACCTACTGAAGCC
AAGAGGGAAAAAATTAAGACTGATTAAACAAGCCAAACTTATACAACTGATGGGAAATCTTTTGAATAGTGGATTTAATT
TAGGAGAAGTGATAAATTTCTTATCTTTATCAAAACTTGTGGAAAAAGAATTTACATTGAAAATGAAAGAAGGTCTCGCT
TCTGGTCAAGCTTTATCAGAGCTTTTAGAAAGTCTTTCATTTTCTAAAAATGTGGTGACACAACTTGCTTTGGTAGAAGT
GCATGGTAACTTGTCAGGGACGATGCAATTGGTTGAACTTCATCTAAAAAAACAGCTTAAAGTTAAAAATAAATTAGTGG
AAGTTGCTACTTATCCAATATTGTTATTAATTTTTCTGGTTGGAATTATGTGGGGCTTAAAAAATTATTTACTACCACAA
CTCAATAGTGGCAGTAACTTTGCTACTCTATTGATTAATCATTTACCCTTAGTATTTTTTTCGTTTGGAGCTTTTATATT
TTTACTGACAGCTTTGTCAGTAACTCTTTTTAAACGAAAATCAGCAATAATGAATTTTACATTTCTTGTTAAATTACCTC
TGGTTCATTCTTTTATCCGTTTATATTTAACAGCTTACTTTGCAAGAGAATGGGGAAATCTAATTGCTCAAGGTGTCGAA
TTACGTCAAATTATTAACTTGATGAAAAAACAAAAAAGTCGAATTTTTTCAGAAGTTGGCAAGAATCTTGATTTAGAATT
GAATGCTGGTCGGTCTTTTGAACAAGCTGTCAGTAAACTTGCGCTTTTTTTACCCGAGCTCTCATTAATGATTGAATATG
GAGCTATTAAAGATAAATTAGGCTTAGAGCTTTCGCTTTATGCGGATGAATGTTGGGAACAATTTTTTACAAAGATAGAT
CGTTTAATGCAATTAATCCAACCTCTTGTTTTTATTTTTGTAGCATTGATGATTATTTTGCTATATGCAGCCATGTTACT
CCCCATCTATTCAAATATGGGTTCGGGAATATAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (227-348)

• Type II secretion system (T2SS), protein F (T2SSF) (41-159)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGB	Lactococcus lactis subsp. cremoris KW2	83.284	95.518	0.796
comYB	Streptococcus gordonii str. Challis substr. CH1	53.666	95.518	0.513
comYB	Streptococcus mutans UA140	53.079	95.518	0.507
comYB	Streptococcus mutans UA159	52.786	95.518	0.504
comGB/cglB	Streptococcus pneumoniae TIGR4	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae D39	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae R6	51.343	93.838	0.482
comGB/cglB	Streptococcus pneumoniae Rx1	51.343	93.838	0.482
comGB/cglB	Streptococcus mitis SK321	51.045	93.838	0.479
comGB/cglB	Streptococcus mitis NCTC 12261	50.149	93.838	0.471