Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   MIS45_RS08745 Genome accession   NZ_CP092361
Coordinates   1793453..1794739 (-) Length   428 a.a.
NCBI ID   WP_249450248.1    Uniprot ID   -
Organism   Wielerella bovis strain CCUG 44465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1788453..1799739
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS45_RS08715 (MIS45_08715) murB 1788894..1789925 (-) 1032 WP_249450244.1 UDP-N-acetylmuramate dehydrogenase -
  MIS45_RS08720 (MIS45_08720) - 1789941..1790561 (-) 621 WP_249450245.1 L-threonylcarbamoyladenylate synthase -
  MIS45_RS08725 (MIS45_08725) pncC 1790635..1791114 (-) 480 WP_249450246.1 nicotinamide-nucleotide amidase -
  MIS45_RS08730 (MIS45_08730) zapD 1791167..1791913 (-) 747 WP_249442248.1 cell division protein ZapD -
  MIS45_RS08735 (MIS45_08735) coaE 1791926..1792540 (-) 615 WP_249450247.1 dephospho-CoA kinase -
  MIS45_RS08740 (MIS45_08740) - 1792544..1793443 (-) 900 Protein_1700 A24 family peptidase -
  MIS45_RS08745 (MIS45_08745) pilG 1793453..1794739 (-) 1287 WP_249450248.1 type II secretion system F family protein Machinery gene
  MIS45_RS08750 (MIS45_08750) pilF 1794764..1796443 (-) 1680 WP_249450249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS45_RS08755 (MIS45_08755) - 1796598..1797353 (-) 756 WP_249450250.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  MIS45_RS08760 (MIS45_08760) - 1797350..1797730 (-) 381 WP_249450251.1 CrcB family protein -
  MIS45_RS08765 (MIS45_08765) - 1797727..1798548 (-) 822 WP_249450252.1 ferritin-like domain-containing protein -
  MIS45_RS08770 (MIS45_08770) lolA 1798745..1799326 (+) 582 WP_249451368.1 outer membrane lipoprotein chaperone LolA -

Sequence

Protein


Download         Length: 428 a.a.        Molecular weight: 46994.32 Da        Isoelectric Point: 9.8413

>NTDB_id=656780 MIS45_RS08745 WP_249450248.1 1793453..1794739(-) (pilG) [Wielerella bovis strain CCUG 44465]
MAEARKSAKSAAKKPVKKTAAKSAKPEKKEKGNRYQFEGKNLQTENIVRGEVVAKNEDEARQKLARRSIKVMQLTKMKKA
RAKKITQADITIFTRQLSTMMKAGLPMMQAFDIVAKGNPNANMTNLLMDIRNRVEQGESLGDSFAEHPAYFDKFYCNLVA
AGEAGGVLEGLLDKLATYKEKTQAIKKKVKGALTYPIAVMVVAVALILVMMMFVLPSFKKIYEGLGAELPGLTQWMMNAS
DFFVEKGWIIIPGLILGTWGLIQFHKHSFAFQKRVDSWLLKMPIFGEIVMKATIARWSRTTATLFTAGVPLVEVLDSVAG
ACGNIIYEEATHEIRSKVNQGISLTSGMQGANVFPNMVVQMASIGEESGALDDMLNKVAEFYEEEVDTAVATLSSLMEPI
IMVVLGSIVGVILVAMYLPLFSMGDAIG

Nucleotide


Download         Length: 1287 bp        

>NTDB_id=656780 MIS45_RS08745 WP_249450248.1 1793453..1794739(-) (pilG) [Wielerella bovis strain CCUG 44465]
ATGGCAGAAGCAAGAAAATCGGCAAAATCCGCCGCGAAAAAGCCAGTGAAAAAAACTGCAGCAAAATCAGCAAAACCTGA
AAAGAAAGAAAAAGGTAACCGCTATCAATTTGAAGGTAAGAACTTACAGACTGAAAATATTGTTCGCGGTGAGGTGGTTG
CAAAAAACGAAGATGAGGCACGTCAGAAGTTAGCGCGTCGCTCCATTAAAGTGATGCAATTAACCAAAATGAAAAAGGCG
CGTGCGAAGAAAATCACACAAGCTGATATTACCATCTTTACTCGCCAACTTTCTACTATGATGAAAGCGGGTTTGCCCAT
GATGCAAGCATTTGATATTGTGGCAAAAGGTAATCCAAATGCGAATATGACAAATTTGTTGATGGATATTCGTAACCGAG
TTGAGCAAGGTGAATCTTTGGGTGATTCCTTTGCTGAACATCCTGCGTATTTTGATAAATTTTATTGCAATTTGGTTGCA
GCAGGCGAAGCTGGTGGTGTATTGGAAGGTTTGCTGGATAAATTAGCAACCTATAAAGAAAAAACGCAAGCCATTAAGAA
AAAAGTGAAAGGTGCTTTAACATATCCCATTGCAGTAATGGTGGTTGCAGTTGCTTTGATTTTGGTAATGATGATGTTTG
TATTGCCATCGTTTAAGAAAATTTACGAAGGCTTGGGAGCTGAATTACCAGGTTTAACACAATGGATGATGAATGCTTCT
GATTTTTTTGTGGAAAAAGGTTGGATTATTATCCCTGGATTAATTTTGGGTACTTGGGGCTTAATTCAATTTCATAAACA
TTCTTTTGCTTTCCAAAAACGGGTAGACAGTTGGTTATTAAAAATGCCTATTTTTGGTGAGATTGTGATGAAAGCTACCA
TTGCACGCTGGTCGCGTACAACTGCAACCTTGTTTACAGCAGGTGTACCATTAGTTGAAGTATTGGATTCAGTGGCAGGT
GCCTGCGGTAATATTATTTACGAGGAAGCCACACATGAAATTCGCAGCAAAGTGAACCAAGGTATTTCTTTGACTTCGGG
GATGCAAGGTGCCAATGTATTCCCAAATATGGTGGTGCAAATGGCATCTATTGGTGAAGAATCGGGTGCTTTGGATGATA
TGTTGAACAAAGTAGCAGAATTTTATGAAGAAGAAGTGGATACTGCTGTTGCTACTCTATCTTCTTTGATGGAACCAATT
ATTATGGTAGTTTTGGGTAGTATTGTCGGTGTTATTCTGGTGGCGATGTACTTGCCACTGTTCAGCATGGGCGATGCCAT
CGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(297-419)

Type II secretion system (T2SS), protein F (T2SSF)

(93-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

62.75

93.458

0.586
  pilG Neisseria meningitidis 44/76-A

62

93.458

0.579
  pilC Legionella pneumophila strain ERS1305867

47.236

92.991

0.439
  pilC Pseudomonas stutzeri DSM 10701

43.564

94.393

0.411
  pilC Acinetobacter baumannii D1279779

44.675

89.953

0.402
  pilC Acinetobacter baylyi ADP1

43.928

90.421

0.397