Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   MIS45_RS08750 Genome accession   NZ_CP092361
Coordinates   1794764..1796443 (-) Length   559 a.a.
NCBI ID   WP_249450249.1    Uniprot ID   -
Organism   Wielerella bovis strain CCUG 44465     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1789764..1801443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS45_RS08720 (MIS45_08720) - 1789941..1790561 (-) 621 WP_249450245.1 L-threonylcarbamoyladenylate synthase -
  MIS45_RS08725 (MIS45_08725) pncC 1790635..1791114 (-) 480 WP_249450246.1 nicotinamide-nucleotide amidase -
  MIS45_RS08730 (MIS45_08730) zapD 1791167..1791913 (-) 747 WP_249442248.1 cell division protein ZapD -
  MIS45_RS08735 (MIS45_08735) coaE 1791926..1792540 (-) 615 WP_249450247.1 dephospho-CoA kinase -
  MIS45_RS08740 (MIS45_08740) - 1792544..1793443 (-) 900 Protein_1700 A24 family peptidase -
  MIS45_RS08745 (MIS45_08745) pilG 1793453..1794739 (-) 1287 WP_249450248.1 type II secretion system F family protein Machinery gene
  MIS45_RS08750 (MIS45_08750) pilF 1794764..1796443 (-) 1680 WP_249450249.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIS45_RS08755 (MIS45_08755) - 1796598..1797353 (-) 756 WP_249450250.1 FKBP-type peptidyl-prolyl cis-trans isomerase -
  MIS45_RS08760 (MIS45_08760) - 1797350..1797730 (-) 381 WP_249450251.1 CrcB family protein -
  MIS45_RS08765 (MIS45_08765) - 1797727..1798548 (-) 822 WP_249450252.1 ferritin-like domain-containing protein -
  MIS45_RS08770 (MIS45_08770) lolA 1798745..1799326 (+) 582 WP_249451368.1 outer membrane lipoprotein chaperone LolA -
  MIS45_RS08775 (MIS45_08775) - 1799539..1800642 (+) 1104 WP_249451369.1 anhydro-N-acetylmuramic acid kinase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62034.88 Da        Isoelectric Point: 5.3211

>NTDB_id=656781 MIS45_RS08750 WP_249450249.1 1794764..1796443(-) (pilF) [Wielerella bovis strain CCUG 44465]
MSVGLLRVLVQQKLVPQDKIEKYQTAIKENKPVLPLVFGDKIISPEALAELCSSLFNYPMLDLNYFPRSAIVQDLIDEAQ
MQELRAIPVLKRGRKVFFAVSDPTQIQNVQKAVFASGLQVDLVIARDDQLTTVLEWFGQSNSALLKEMGMEVNKEEAKQT
MIVDGQEEEDGPVARFIQKILHDAVTSGASDIHFEFYEFMARVRFRTDGVLREVVQPPLQLRSQLASRIKVMAKLDISEK
RVPQDGRIQLQFSKNTKAIDFRVNTMPCLFGEKVVMRILNSDAASLNIDQLGFEPFQKELIMEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTDDINISTAEDPAEINLPGINQVNVNDKQGLTFEAALKAFLRQDPDVIMIGEIRDLGTADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASAVNLIMAQRLARRLCSACKAPMERPPQKALLEVGFTEEDLAQDWTM
YRPVGCDACKGKGYKGRAGLYEVMKMSEKMKAVIMSGGTEVDIANIAYEDGLVDLRRSGLLKVMQGITSLEEVIATTNE

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=656781 MIS45_RS08750 WP_249450249.1 1794764..1796443(-) (pilF) [Wielerella bovis strain CCUG 44465]
ATGAGCGTAGGTTTATTGCGGGTCTTGGTGCAACAAAAATTAGTCCCACAAGATAAAATTGAAAAATATCAAACGGCTAT
CAAAGAAAACAAACCTGTTTTACCTTTGGTGTTTGGCGATAAAATTATTTCGCCTGAAGCATTGGCAGAATTGTGTTCGT
CTTTATTCAACTATCCGATGTTGGATTTAAACTATTTTCCACGCAGTGCGATTGTGCAAGATTTGATTGATGAAGCACAA
ATGCAAGAATTGCGTGCGATTCCTGTGTTAAAACGTGGTCGAAAAGTGTTCTTCGCTGTTTCAGATCCTACACAAATTCA
AAATGTACAAAAAGCGGTATTTGCCAGCGGTTTGCAAGTGGATTTGGTCATCGCGCGTGATGACCAATTAACTACTGTGT
TGGAATGGTTTGGTCAAAGTAACTCTGCTTTGCTCAAAGAAATGGGCATGGAGGTGAATAAAGAAGAAGCCAAACAGACT
ATGATTGTGGATGGGCAGGAAGAAGAAGATGGTCCTGTCGCACGGTTTATTCAAAAAATCTTGCATGACGCGGTTACTTC
TGGTGCATCTGATATTCACTTTGAGTTCTACGAATTTATGGCGCGTGTACGTTTCCGTACCGATGGTGTATTGCGTGAAG
TGGTGCAACCACCCTTACAATTGCGTAGCCAGTTGGCATCGCGTATTAAAGTAATGGCAAAATTGGATATTTCTGAAAAA
CGTGTACCGCAGGACGGACGTATTCAGTTGCAATTTTCTAAAAATACCAAAGCGATTGACTTTCGTGTGAACACCATGCC
GTGTTTGTTTGGTGAAAAAGTCGTGATGCGTATTTTGAACTCAGATGCAGCCAGCCTGAATATTGACCAGTTGGGTTTTG
AACCTTTCCAAAAAGAATTAATTATGGAAGCGATTCATCGTCCATACGGCATGGTGTTGGTAACAGGTCCAACGGGTTCG
GGTAAAACGGTTTCTCTCTATACTTGTTTGAACATTCTGAATACCGATGACATCAATATTTCTACTGCAGAAGACCCTGC
GGAGATTAACTTACCAGGGATTAACCAAGTAAACGTAAACGATAAACAAGGTTTAACTTTTGAGGCAGCCTTAAAAGCAT
TCCTGCGTCAAGACCCTGATGTGATTATGATTGGTGAGATTCGCGACTTAGGTACGGCGGATATTGCGATTAAAGCAGCG
CAAACAGGTCATATGGTGTTCTCAACCTTACACACCAATAATGCACCAGCTACTTTGTCGCGTATGTTGAATATGGGCGT
TGCCCCATTTAATATTGCTTCGGCGGTGAACTTGATTATGGCACAACGTTTGGCACGTCGTTTGTGTTCTGCTTGCAAAG
CACCAATGGAGCGTCCACCTCAAAAGGCTTTATTAGAAGTAGGCTTTACTGAGGAGGATTTAGCGCAAGATTGGACAATG
TATCGTCCTGTGGGTTGTGATGCTTGTAAAGGTAAAGGCTATAAAGGTCGTGCAGGTTTGTATGAAGTAATGAAAATGTC
TGAAAAAATGAAAGCTGTCATCATGAGTGGTGGTACGGAGGTGGATATTGCTAATATCGCATATGAAGATGGTTTGGTAG
ATTTGCGTCGTTCAGGTTTACTGAAAGTTATGCAAGGTATTACTTCTTTGGAAGAAGTTATTGCAACCACAAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(41-140)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

69.231

100

0.692
  pilB Acinetobacter baumannii D1279779

53.603

100

0.546
  pilB Acinetobacter baylyi ADP1

52.289

100

0.531
  pilB Legionella pneumophila strain ERS1305867

47.679

100

0.478
  pilB Vibrio cholerae strain A1552

46.372

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

46.29

100

0.469
  pilB Vibrio campbellii strain DS40M4

47.793

93.202

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.458

92.487

0.365