Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MIS33_RS05710 Genome accession   NZ_CP092359
Coordinates   1148857..1150041 (+) Length   394 a.a.
NCBI ID   WP_283397256.1    Uniprot ID   -
Organism   Wielerella bovis strain 08LB461     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1143857..1155041
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIS33_RS05680 (MIS33_05680) - 1144648..1145442 (-) 795 WP_249446338.1 c-type cytochrome -
  MIS33_RS05690 (MIS33_05690) - 1146142..1146510 (+) 369 WP_249445190.1 hemerythrin domain-containing protein -
  MIS33_RS05695 (MIS33_05695) - 1146546..1146980 (+) 435 WP_249441704.1 META domain-containing protein -
  MIS33_RS05700 (MIS33_05700) folP 1147001..1147846 (+) 846 WP_249446278.1 dihydropteroate synthase -
  MIS33_RS05705 (MIS33_05705) - 1147914..1148684 (+) 771 WP_249441706.1 ADP-ribosylglycohydrolase family protein -
  MIS33_RS05710 (MIS33_05710) pilU 1148857..1150041 (+) 1185 WP_283397256.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MIS33_RS05715 (MIS33_05715) - 1150459..1151145 (-) 687 WP_249441707.1 sulfite exporter TauE/SafE family protein -
  MIS33_RS05720 (MIS33_05720) - 1151377..1151985 (+) 609 WP_249446277.1 nitroreductase family protein -
  MIS33_RS05725 (MIS33_05725) - 1152115..1154001 (+) 1887 WP_249451591.1 lytic transglycosylase domain-containing protein -
  MIS33_RS05730 (MIS33_05730) grxB 1154076..1154726 (+) 651 WP_249446275.1 glutaredoxin 2 -
  MIS33_RS05735 (MIS33_05735) - 1154796..1155032 (+) 237 WP_249446274.1 RNA-binding S4 domain-containing protein -

Sequence

Protein


Download         Length: 394 a.a.        Molecular weight: 43958.39 Da        Isoelectric Point: 5.6463

>NTDB_id=656721 MIS33_RS05710 WP_283397256.1 1148857..1150041(+) (pilU) [Wielerella bovis strain 08LB461]
MSALPPVNEEMKKFIHSLLTHMVKNKGSDLFITAGFPPAMKLDGKLTRLSDKPLTAEHTMQIARSIMDDKQAEEFLNTNE
CNFAISLAGVSRFRINAMIQRGAAALVCRVITSDIPKFEDLKLPPVLRDVVMEKRGLVIFVGGTGSGKSTSLAAMIDYRN
ENSFGHIITIEDPIEYVHPHKNCIVSQREVGVDTENWFAALKNTLRQAPDVILIGEIRDRETMDYALAFAETGHLCMATL
HANNSNQALDRIINFFPEERRTQLLTDLSLNLKGFISQRLIPRKTGKGRSAAVEILLNSPLISELILKGEVHGIKEIMAK
SRELGMQTFDQALFDLYEDDMISYDDAIKNADSANDLRLQIKLNSKKNATAGEDGALDGLSIMDYQHEQPDEQQ

Nucleotide


Download         Length: 1185 bp        

>NTDB_id=656721 MIS33_RS05710 WP_283397256.1 1148857..1150041(+) (pilU) [Wielerella bovis strain 08LB461]
ATGTCCGCACTCCCACCCGTAAACGAAGAAATGAAAAAGTTCATTCACAGCTTATTGACCCACATGGTCAAAAACAAAGG
CTCGGACTTATTCATCACCGCAGGCTTCCCACCAGCCATGAAATTGGACGGCAAATTAACCCGTTTGAGCGATAAACCGC
TGACCGCTGAACACACCATGCAAATCGCGCGTTCCATTATGGACGACAAACAAGCCGAAGAATTTTTAAACACCAACGAA
TGCAACTTCGCCATCAGTTTGGCTGGTGTATCTCGTTTCCGTATCAATGCCATGATTCAACGTGGCGCAGCCGCTTTGGT
TTGCCGTGTGATTACCAGTGATATTCCCAAATTTGAAGATTTAAAATTGCCGCCTGTATTGCGCGATGTGGTTATGGAAA
AACGCGGCTTAGTCATTTTTGTTGGTGGTACGGGCTCGGGTAAATCCACATCGCTTGCTGCCATGATTGATTATCGCAAT
GAAAACAGCTTTGGTCATATCATTACCATTGAAGACCCGATTGAATATGTGCATCCGCATAAAAATTGCATCGTTTCACA
ACGTGAAGTTGGCGTAGATACTGAAAACTGGTTTGCAGCCTTGAAAAATACCCTGCGTCAAGCACCTGATGTGATTCTGA
TTGGCGAGATTCGCGACCGTGAAACCATGGACTATGCGCTTGCCTTTGCCGAAACAGGACATTTGTGTATGGCAACCTTG
CACGCCAACAACTCTAACCAAGCACTTGACCGTATCATCAACTTCTTCCCCGAAGAACGCCGCACCCAGTTGCTGACTGA
CTTATCTTTGAACTTAAAAGGTTTCATTTCACAACGTTTGATTCCGCGTAAAACAGGTAAAGGTCGTTCAGCGGCAGTGG
AAATTTTGTTGAACTCGCCTTTGATTTCTGAACTGATTTTGAAAGGCGAAGTGCATGGCATTAAAGAAATCATGGCAAAA
TCACGCGAATTGGGCATGCAAACTTTTGACCAAGCCTTGTTTGATTTGTATGAAGACGACATGATTTCCTATGACGATGC
GATTAAAAATGCCGACTCTGCCAATGACTTGCGTTTACAAATTAAATTGAACAGTAAGAAAAATGCCACAGCTGGCGAAG
ATGGGGCATTGGACGGCTTGTCTATTATGGATTATCAGCACGAACAGCCTGATGAGCAGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(125-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

60.795

89.34

0.543
  pilU Acinetobacter baylyi ADP1

59.659

89.34

0.533
  pilU Vibrio cholerae strain A1552

55.096

92.132

0.508
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.899

90.863

0.381
  pilT Legionella pneumophila strain Lp02

43.243

84.518

0.365
  pilT Legionella pneumophila strain ERS1305867

43.243

84.518

0.365
  pilT Pseudomonas aeruginosa PAK

42.433

85.533

0.363