Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MIH19_RS04885 Genome accession   NZ_CP092289
Coordinates   1024328..1026043 (-) Length   571 a.a.
NCBI ID   WP_249007806.1    Uniprot ID   -
Organism   Marinobacter sp. M1C     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019328..1031043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIH19_RS04855 (MIH19_04855) yacG 1019448..1019645 (-) 198 WP_249007800.1 DNA gyrase inhibitor YacG -
  MIH19_RS04860 (MIH19_04860) tsaA 1019722..1020477 (-) 756 WP_249007801.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MIH19_RS04865 (MIH19_04865) - 1020483..1021592 (-) 1110 WP_249007802.1 hypothetical protein -
  MIH19_RS04870 (MIH19_04870) coaE 1021564..1022172 (-) 609 WP_249007803.1 dephospho-CoA kinase -
  MIH19_RS04875 (MIH19_04875) pilD 1022229..1023104 (-) 876 WP_249007804.1 A24 family peptidase Machinery gene
  MIH19_RS04880 (MIH19_04880) pilC 1023108..1024325 (-) 1218 WP_249007805.1 type II secretion system F family protein Machinery gene
  MIH19_RS04885 (MIH19_04885) pilB 1024328..1026043 (-) 1716 WP_249007806.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIH19_RS04890 (MIH19_04890) tviB 1026287..1027549 (+) 1263 WP_249007807.1 Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB -
  MIH19_RS04895 (MIH19_04895) - 1027876..1028982 (+) 1107 WP_249007808.1 UDP-N-acetylglucosamine 2-epimerase -
  MIH19_RS04900 (MIH19_04900) - 1028999..1030150 (+) 1152 WP_249007809.1 CDP-glycerol glycerophosphotransferase family protein -
  MIH19_RS04905 (MIH19_04905) - 1030151..1030585 (-) 435 WP_249007810.1 adenylyltransferase/cytidyltransferase family protein -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62474.63 Da        Isoelectric Point: 5.2374

>NTDB_id=656565 MIH19_RS04885 WP_249007806.1 1024328..1026043(-) (pilB) [Marinobacter sp. M1C]
MTINTSNITLTGLARRFVSDGLLDESTAKDAFFQASQNRIPLITYLTQHKLANSSALAFSAAMEFGISVLDLDAFLPEMM
PDKLVDEKLLRKHNALPLYKRGNRLFIAVSDPTNVQALDEIKFNTGLSTDAILVDDSKLRAAIEKYLASSESSMGDLADT
DLEGIETEGADGNEDDGAVVVAEVDDAPIVKYVNKMLLDAIRGGSSDVHFEPYEKTYRVRFRTDGILKEMSRPSIKLAPK
ISARIKIMAQLDISERRLPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDALGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTDERNISTAEDPAEINLEGVNQVNVNNKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQAGDIPKDVLLAE
GFSTEQIETGITLYHPKGCDKCTNGYKGRVGIYEVVKVTEQLASMIMEEASSIKIAKAAQAEGFRTLRQSALLKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=656565 MIH19_RS04885 WP_249007806.1 1024328..1026043(-) (pilB) [Marinobacter sp. M1C]
ATGACAATAAACACAAGCAACATCACCTTAACGGGTCTGGCACGGCGCTTTGTAAGCGACGGCTTGCTGGATGAATCCAC
GGCCAAAGACGCCTTTTTTCAGGCTTCTCAGAACCGTATTCCGCTGATTACCTACCTAACTCAGCACAAGTTGGCAAACA
GCTCGGCGTTGGCGTTTTCGGCGGCCATGGAGTTTGGTATTTCAGTGCTGGACCTGGATGCGTTTTTGCCGGAGATGATG
CCCGACAAGCTGGTGGACGAAAAACTGCTGCGCAAGCACAACGCCCTGCCCCTGTATAAGCGTGGCAATCGGCTGTTTAT
TGCAGTAAGTGACCCCACCAACGTTCAGGCTCTGGACGAAATCAAGTTCAATACCGGCTTAAGCACCGATGCAATTCTGG
TGGACGATTCCAAGCTGCGCGCGGCTATCGAAAAGTATTTGGCGTCTAGTGAAAGCTCCATGGGCGACCTGGCCGATACA
GACCTTGAAGGTATCGAAACAGAAGGCGCGGATGGAAACGAAGACGACGGTGCAGTGGTTGTTGCCGAAGTAGACGATGC
ACCCATCGTAAAGTATGTGAACAAAATGCTGCTAGATGCGATTCGCGGGGGTTCTTCAGACGTTCACTTTGAACCTTACG
AGAAAACCTACCGCGTACGCTTTCGCACTGACGGCATTCTAAAAGAAATGTCGCGACCCTCTATAAAGCTGGCACCGAAA
ATATCCGCGCGAATAAAGATCATGGCGCAGCTGGACATTTCCGAGCGACGTTTGCCGCAAGACGGCCGCATAAAAATGAG
GCTGTCCAAAACCAAGTCCATCGACTTTCGGGTGAACACCCTGCCCACGTTGTGGGGCGAGAAAATCGTGCTGCGTATTC
TGGACCCCAGCCAGGCAAAGTTGGGCATTGACGCCCTAGGGTATGAAGAAGACCAGAAGAAGCTGTATATGGACGCCCTG
GCGCAACCCCAAGGCATGATTCTGGTCACGGGGCCCACCGGTTCCGGTAAAACCGTATCTCTGTATACCGGTTTGAACAT
TCTGAACACCGACGAGCGCAATATTTCCACCGCGGAAGACCCGGCGGAAATTAACCTGGAAGGCGTGAATCAGGTCAACG
TGAACAACAAAGTGGGGCTTGGCTTCGCCGAAGCGCTACGGGCGTTTTTGCGGCAAGACCCTGACGTTATCATGGTGGGC
GAGATTCGAGACCTGGAAACCGCCAACATTGCCATTAAAGCGGCACAAACCGGTCACCTTGTGTTATCCACGCTGCACAC
CAACAGCGCGGCCGAAACCTTAACCCGCATGATGAACATGGGCGTGCCGGCGTTTAACATTGCCACGTCTGTTAGCCTGA
TTATTGCACAGCGCCTGGGCCGGCGACTTTGCAGCTGTAAGCAGGCCGGAGACATCCCAAAAGACGTTCTTTTAGCCGAA
GGGTTCTCAACTGAACAAATTGAAACAGGGATCACGTTGTACCATCCGAAGGGCTGTGATAAATGCACTAATGGGTATAA
AGGCCGTGTCGGCATTTACGAGGTGGTGAAAGTAACCGAGCAGCTGGCCAGTATGATTATGGAAGAGGCCAGTTCTATCA
AGATTGCAAAAGCAGCTCAGGCTGAAGGCTTCCGAACCCTGCGCCAGTCCGCCCTGTTAAAAGTGATTGAGGGTGTGACC
AGCCTTGAAGAAGCCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(195-462)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.46

98.949

0.559
  pilB Acinetobacter baylyi ADP1

54.45

100

0.546
  pilB Legionella pneumophila strain ERS1305867

53.206

100

0.538
  pilB Vibrio parahaemolyticus RIMD 2210633

51.899

96.848

0.503
  pilB Vibrio campbellii strain DS40M4

50.796

98.949

0.503
  pilB Vibrio cholerae strain A1552

54.335

90.893

0.494
  pilF Neisseria gonorrhoeae MS11

46.903

98.949

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

41.892

90.718

0.38