Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   OG904_RS01425 Genome accession   NZ_CP108221
Coordinates   312102..313214 (+) Length   370 a.a.
NCBI ID   WP_405486491.1    Uniprot ID   -
Organism   Streptomyces sp. NBC_00096     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 307102..318214
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OG904_RS01410 (OG904_01405) - 307248..307682 (+) 435 WP_405486485.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OG904_RS01415 (OG904_01410) - 307679..308203 (+) 525 WP_405486487.1 type II secretion system protein -
  OG904_RS01420 (OG904_01415) - 308272..312105 (+) 3834 WP_405486489.1 hypothetical protein -
  OG904_RS01425 (OG904_01420) pilT 312102..313214 (+) 1113 WP_405486491.1 type IV pilus twitching motility protein PilT Machinery gene
  OG904_RS01430 (OG904_01425) - 313108..313440 (-) 333 WP_405497694.1 DUF2867 domain-containing protein -
  OG904_RS01440 (OG904_01435) - 313656..313949 (-) 294 WP_405486493.1 hypothetical protein -
  OG904_RS01445 (OG904_01440) - 314278..315144 (-) 867 WP_405486495.1 universal stress protein -
  OG904_RS01450 (OG904_01445) - 315144..316046 (-) 903 WP_405486497.1 universal stress protein -
  OG904_RS01455 (OG904_01450) - 316333..316956 (+) 624 WP_405486499.1 CBS domain-containing protein -
  OG904_RS01460 (OG904_01455) - 316995..317867 (+) 873 WP_405486501.1 universal stress protein -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 40098.61 Da        Isoelectric Point: 6.3895

>NTDB_id=656563 OG904_RS01425 WP_405486491.1 312102..313214(+) (pilT) [Streptomyces sp. NBC_00096]
MSTHTTLETTMPGPSSAIIDHLLGELWERGGSDLLLTTGSAPFLRVDGALRPVEGAALTEPEADRLVTGVLGKELTERFR
REKQVDFAFSWGEKARLRGNAFVQRGASALALRIIPFDVPSSEQLGLPPAVMGWAGMPRGLVLVTGPTGSGKSTSLAALL
DYVNTHRSVHILTIEDPIEYLHQHKRSAVNQREIGTDTDSFHAALRAALREDPDVLLLGEMRDPESISAALTIAETGHLV
FATLHTNDTSQTLDRIVDVFPAEQQPQIRLQLAHTLVGILNQTLIPRTGGGRVAAFEVLAGTSAIRNLIREGKTRQIRNM
VATGHRDGMQTLETSINALVAAGTIAYEEAVLHTAYPEDIRRPHVPIGHT

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=656563 OG904_RS01425 WP_405486491.1 312102..313214(+) (pilT) [Streptomyces sp. NBC_00096]
ATGAGTACGCACACCACCTTGGAGACCACCATGCCCGGGCCGAGTTCCGCGATCATCGACCACCTGCTCGGCGAGCTGTG
GGAACGGGGCGGCAGCGACCTGCTGCTCACCACCGGATCGGCGCCGTTCCTCCGCGTCGACGGCGCCCTGCGACCGGTCG
AGGGGGCCGCGCTGACCGAGCCGGAGGCCGATCGCCTGGTGACCGGGGTGCTGGGCAAGGAACTCACGGAGCGGTTCCGC
CGGGAGAAGCAGGTCGATTTCGCGTTCAGCTGGGGGGAGAAGGCCAGGTTGCGCGGGAACGCCTTCGTGCAGCGCGGCGC
GTCGGCGCTGGCGCTGCGGATCATCCCGTTCGACGTGCCGTCGTCCGAGCAGCTCGGGCTCCCCCCGGCCGTCATGGGGT
GGGCGGGCATGCCGCGCGGGTTGGTGCTGGTCACCGGGCCGACCGGGTCGGGGAAGTCGACGAGCCTGGCGGCGCTCCTC
GACTACGTCAACACGCACCGGTCGGTGCACATCCTCACCATCGAGGACCCGATCGAGTACCTCCACCAGCACAAGCGCTC
CGCGGTCAACCAGCGTGAGATCGGCACCGACACCGACTCGTTCCACGCCGCGCTGCGCGCGGCCCTGCGCGAGGATCCCG
ACGTCCTGCTCCTCGGCGAGATGCGCGACCCGGAGAGCATCTCCGCAGCCCTGACCATCGCCGAGACCGGACACCTGGTG
TTCGCCACCCTGCACACCAACGACACGTCGCAGACGCTGGACCGGATCGTCGACGTGTTCCCGGCCGAGCAACAGCCGCA
GATCCGGCTGCAATTGGCGCACACCCTGGTCGGCATCCTCAACCAGACGCTCATCCCCCGGACCGGCGGGGGCCGGGTGG
CCGCCTTCGAGGTGCTGGCAGGAACGTCGGCGATCCGCAACCTGATCCGCGAGGGCAAGACCCGCCAGATCCGCAACATG
GTCGCGACCGGCCACCGTGACGGCATGCAGACCCTCGAAACCAGCATCAACGCCCTCGTCGCGGCGGGCACCATCGCCTA
CGAGGAGGCCGTGCTGCACACGGCCTACCCGGAGGACATCCGGCGGCCCCACGTCCCGATCGGCCACACCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(137-260)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

48.397

92.703

0.449
  pilT Pseudomonas aeruginosa PAK

48.246

92.432

0.446
  pilT Acinetobacter baumannii D1279779

47.941

91.892

0.441
  pilT Acinetobacter baumannii strain A118

47.941

91.892

0.441
  pilT Neisseria meningitidis 8013

48.503

90.27

0.438
  pilT Acinetobacter nosocomialis M2

47.647

91.892

0.438
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.214

90.811

0.438
  pilT Neisseria gonorrhoeae MS11

47.748

90

0.43
  pilT Legionella pneumophila strain ERS1305867

47.041

91.351

0.43
  pilT Legionella pneumophila strain Lp02

47.041

91.351

0.43
  pilT Acinetobacter baylyi ADP1

48.318

88.378

0.427
  pilT Vibrio cholerae strain A1552

47.59

89.73

0.427
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

47.59

89.73

0.427
  pilU Pseudomonas stutzeri DSM 10701

42.319

93.243

0.395
  pilU Vibrio cholerae strain A1552

40.237

91.351

0.368
  pilU Acinetobacter baylyi ADP1

38.841

93.243

0.362