Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   MIH18_RS08630 Genome accession   NZ_CP092284
Coordinates   1868184..1870301 (-) Length   705 a.a.
NCBI ID   WP_249014328.1    Uniprot ID   -
Organism   Marinobacter sp. M3C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1863184..1875301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIH18_RS08615 (MIH18_08610) - 1864782..1866368 (-) 1587 WP_249014326.1 AAA family ATPase -
  MIH18_RS08620 (MIH18_08615) aroB 1866394..1867485 (-) 1092 WP_249014327.1 3-dehydroquinate synthase -
  MIH18_RS08625 (MIH18_08620) aroK 1867478..1868044 (-) 567 WP_249007654.1 shikimate kinase AroK -
  MIH18_RS08630 (MIH18_08625) pilQ 1868184..1870301 (-) 2118 WP_249014328.1 type IV pilus secretin PilQ Machinery gene
  MIH18_RS08635 (MIH18_08630) - 1870301..1870873 (-) 573 WP_249007652.1 pilus assembly protein PilP -
  MIH18_RS08640 (MIH18_08635) pilO 1870876..1871487 (-) 612 WP_249007651.1 type 4a pilus biogenesis protein PilO -
  MIH18_RS08645 (MIH18_08640) - 1871484..1872050 (-) 567 WP_249007650.1 PilN domain-containing protein -
  MIH18_RS08650 (MIH18_08645) pilM 1872050..1873114 (-) 1065 WP_249007649.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75456.02 Da        Isoelectric Point: 4.3801

>NTDB_id=656507 MIH18_RS08630 WP_249014328.1 1868184..1870301(-) (pilQ) [Marinobacter sp. M3C]
MNNSNMNAQKCLKPRLAMFKKLNVIVCAVTVGLLSSLASAATLQDVSFSSLPGDRLEVTLQFDGPPPEPTGYTIERPARI
AVDLKDTTSALGARSLPLGSGNAQSMTVVETNDRTRLIFNLIELVPYTTQRNNNALVMIIGGDSADLASGVSGSDGASPV
ASAADAAPALVDVDFRRGAQGEGRVVVRLASTTTQVDLSELGGKIRLTMPDLTVPERLRRRLDVTDFATPVQRIDTFIEG
GGAVIEIRPEGDYDYIAYQSGSEFTVSVERLSEEEAVTRREEKFPYSGDKLSLNFQDIEVRSVLQLIADFTGLNLVASDT
VGGSITLRLQNVPWDQALDLILKTKGLDKRQIGNVLLVAPADEIAARERLELETTKQIAELAPVRLDIIQVNYAKAAEVV
ALVQADQELISSRGFISSDDRTNTISVRETIEKLDEIRRLVSTWDVPVRQVSIEARIVRAQTNVAESLGVSWGGAAYSVS
GNNVISVGGSQSAVEEARNAATGGSGTITFPGALAVDLGVSGDGASSFAIGFGSDDFLVDLELSALESDGKAEIVSQPRV
VTADRQTASIKSGQEIPYQEASSSGATSTSFKEAVLSLEVTPQITPDDKIIMDLEVTQDSRGEDTPSGPAINTNSVTTQV
LVANGETIVLGGIFESTNTETTTKTPFLGDIPYLGRLFKRTQTTELRSELLIFITPKIIKNDLVR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=656507 MIH18_RS08630 WP_249014328.1 1868184..1870301(-) (pilQ) [Marinobacter sp. M3C]
ATGAATAACAGCAACATGAATGCACAAAAATGCTTGAAGCCGAGGCTAGCGATGTTCAAAAAACTCAACGTAATTGTCTG
TGCGGTAACCGTTGGGTTGTTGTCCAGCCTGGCCAGCGCTGCCACTTTGCAGGATGTGTCGTTTTCATCTTTACCTGGGG
ATCGGCTGGAAGTGACTTTGCAGTTTGACGGTCCACCGCCTGAACCTACGGGTTACACCATTGAGCGCCCCGCTCGTATT
GCGGTAGACCTGAAAGACACCACCAGTGCCCTGGGTGCCCGCAGCTTGCCGTTGGGCAGCGGGAATGCGCAAAGCATGAC
CGTTGTTGAAACCAACGACCGTACCCGCCTTATTTTCAACCTGATTGAGCTGGTTCCTTACACCACTCAGCGCAATAATA
ACGCGTTGGTGATGATTATTGGCGGCGATTCCGCAGATTTAGCCAGTGGCGTTTCGGGATCTGACGGTGCCAGCCCTGTT
GCTTCGGCAGCGGACGCGGCGCCGGCGTTGGTAGACGTCGATTTTCGTCGCGGTGCACAAGGCGAAGGCCGTGTGGTTGT
GCGGCTGGCCAGTACGACGACTCAGGTTGACCTGAGCGAACTGGGCGGAAAAATCAGACTGACCATGCCGGATCTGACTG
TGCCCGAGCGCCTGCGTCGCCGTTTAGACGTGACAGATTTTGCCACGCCGGTACAACGGATCGACACCTTTATTGAGGGT
GGCGGTGCGGTTATAGAAATTCGTCCGGAAGGCGACTATGACTACATTGCGTACCAGTCTGGCAGCGAATTTACCGTGAG
CGTTGAACGGCTCAGCGAGGAAGAAGCAGTAACCCGCCGTGAAGAGAAGTTCCCATATTCCGGCGACAAGCTCTCGCTAA
ACTTTCAGGACATTGAAGTTCGCTCGGTGCTTCAGCTGATTGCTGACTTTACCGGTTTGAATCTGGTGGCCAGCGATACC
GTTGGTGGAAGCATCACGCTGCGCTTGCAGAATGTGCCTTGGGATCAGGCGCTGGATCTCATTTTGAAAACCAAAGGCTT
GGATAAGCGCCAGATTGGCAATGTATTACTGGTAGCGCCCGCCGATGAAATCGCCGCTCGTGAACGGCTGGAACTGGAAA
CAACCAAGCAGATCGCCGAGCTTGCCCCAGTGCGTTTGGACATCATTCAGGTCAATTACGCAAAAGCGGCCGAAGTAGTA
GCGTTGGTGCAGGCCGACCAAGAGCTGATTTCAAGCCGCGGTTTCATTTCCTCAGACGACCGAACCAACACCATCAGCGT
GCGCGAAACCATTGAAAAACTGGACGAGATCCGTCGCCTGGTGTCCACCTGGGACGTCCCCGTGCGTCAGGTTTCCATTG
AAGCCCGGATTGTGCGCGCACAAACCAACGTGGCCGAGAGCCTGGGTGTGAGCTGGGGCGGAGCAGCTTACAGCGTGAGT
GGCAACAACGTAATTTCCGTAGGTGGCTCACAATCTGCAGTTGAGGAGGCACGCAACGCTGCGACCGGCGGATCTGGAAC
GATTACTTTCCCTGGGGCGTTAGCGGTAGATCTGGGCGTTAGCGGCGACGGGGCCTCTTCTTTTGCGATTGGTTTTGGCA
GCGATGACTTCCTGGTAGACCTTGAACTGTCTGCGTTGGAAAGTGATGGTAAAGCGGAAATCGTGTCTCAGCCCAGAGTT
GTTACAGCGGACCGCCAGACGGCGTCTATCAAATCGGGTCAGGAAATCCCGTATCAGGAAGCCAGCAGCAGCGGCGCCAC
CAGCACTTCCTTTAAAGAAGCGGTGTTGTCATTGGAGGTAACGCCGCAGATTACACCGGATGACAAAATCATCATGGACC
TTGAGGTGACTCAGGACTCCCGTGGCGAAGACACCCCCAGCGGTCCGGCCATCAACACCAACTCGGTGACAACTCAGGTA
CTGGTCGCCAACGGTGAAACCATTGTTTTGGGCGGTATTTTCGAATCGACCAACACCGAAACCACCACCAAGACACCGTT
CTTGGGCGATATTCCCTACCTGGGCAGGTTGTTCAAGCGCACTCAGACTACCGAGCTGCGTAGCGAACTGCTGATTTTCA
TCACCCCGAAAATCATCAAAAACGACCTGGTTCGTTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(54-137)

Bacterial type II/III secretion system short domain (Secretin_N)

(388-450)

Bacterial type II and III secretion system protein (Secretin)

(545-700)

Secretin and TonB N terminus short domain (STN)

(314-360)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.631

100

0.512
  comQ Acinetobacter baylyi ADP1

42.09

100

0.423
  pilQ Acinetobacter baumannii strain A118

41.111

100

0.42
  pilQ Acinetobacter baumannii D1279779

41.111

100

0.42
  pilQ Legionella pneumophila strain ERS1305867

40.801

95.603

0.39
  pilQ Legionella pneumophila strain Lp02

40.653

95.603

0.389