Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   MIH18_RS08650 Genome accession   NZ_CP092284
Coordinates   1872050..1873114 (-) Length   354 a.a.
NCBI ID   WP_249007649.1    Uniprot ID   -
Organism   Marinobacter sp. M3C     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1867050..1878114
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIH18_RS08625 (MIH18_08620) aroK 1867478..1868044 (-) 567 WP_249007654.1 shikimate kinase AroK -
  MIH18_RS08630 (MIH18_08625) pilQ 1868184..1870301 (-) 2118 WP_249014328.1 type IV pilus secretin PilQ Machinery gene
  MIH18_RS08635 (MIH18_08630) - 1870301..1870873 (-) 573 WP_249007652.1 pilus assembly protein PilP -
  MIH18_RS08640 (MIH18_08635) pilO 1870876..1871487 (-) 612 WP_249007651.1 type 4a pilus biogenesis protein PilO -
  MIH18_RS08645 (MIH18_08640) - 1871484..1872050 (-) 567 WP_249007650.1 PilN domain-containing protein -
  MIH18_RS08650 (MIH18_08645) pilM 1872050..1873114 (-) 1065 WP_249007649.1 pilus assembly protein PilM Machinery gene
  MIH18_RS08655 (MIH18_08650) - 1873320..1875845 (+) 2526 WP_249007648.1 penicillin-binding protein 1A -
  MIH18_RS08660 (MIH18_08655) - 1875971..1877236 (-) 1266 WP_249007647.1 malic enzyme-like NAD(P)-binding protein -
  MIH18_RS08665 (MIH18_08660) rpmE 1877470..1877694 (-) 225 WP_249007646.1 50S ribosomal protein L31 -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37917.96 Da        Isoelectric Point: 4.2135

>NTDB_id=656508 MIH18_RS08650 WP_249007649.1 1872050..1873114(-) (pilM) [Marinobacter sp. M3C]
MFGSFGKKSSAVLGLDISSSSVKLLELSKQGDRFRVESYAVEPLPANAVVEKNITDVEAVGDVIKRVVSKSRSGVKQVAV
AVSGSAVITKVIQMDGGINEFEMEDQIALEADQYIPYPLDEVAIDFEVQGPSESNPDKVDVLIAACRKDNVDVRQDALDI
AGLTTKVVDVEAYALERAYQLIESQLNSHGDDLVVAIMDVGATMATLSVIAEGKTVYTREQVFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYEAEVLNPFREAVVQQVARAMQFFFGASQYNAVDYVVLAGGTSAIPGLVDMVEEKTGTPTLVANP
FADMAVSSRVNASALSNDAPSLMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=656508 MIH18_RS08650 WP_249007649.1 1872050..1873114(-) (pilM) [Marinobacter sp. M3C]
GTGTTTGGATCTTTCGGGAAGAAATCCAGTGCTGTGCTGGGCTTGGACATAAGCTCAAGCTCGGTCAAGCTTTTGGAATT
GTCAAAACAGGGCGACCGCTTTCGGGTTGAAAGTTATGCCGTCGAACCTTTACCTGCAAATGCTGTGGTGGAAAAAAACA
TCACGGATGTTGAAGCCGTAGGTGACGTGATCAAGCGTGTGGTATCGAAATCCCGCTCCGGCGTCAAGCAAGTGGCCGTT
GCCGTGTCCGGTTCCGCGGTGATCACCAAGGTGATCCAGATGGATGGCGGCATCAACGAATTCGAAATGGAAGACCAGAT
TGCCCTGGAGGCAGACCAATACATTCCTTATCCGCTGGATGAAGTCGCGATTGATTTCGAAGTACAGGGCCCCTCTGAAT
CGAACCCGGACAAGGTTGATGTGTTGATTGCGGCTTGCCGCAAAGATAACGTTGATGTGCGCCAAGACGCACTGGATATT
GCCGGCCTGACCACCAAGGTGGTTGACGTAGAGGCTTATGCCCTCGAGCGTGCCTATCAACTGATTGAATCGCAATTGAA
CTCCCACGGCGACGATCTGGTTGTGGCGATCATGGATGTGGGCGCTACCATGGCCACGTTGAGCGTGATTGCCGAAGGCA
AAACTGTGTATACCCGCGAACAGGTCTTTGGTGGCAAACAATTAACCGAAGAAATCCAGCGTCGCTACGGTTTGTCGCTG
GAGGAAGCCGGCCTGGCCAAAAAGCAGGGTGGTTTGCCCGATGACTATGAGGCTGAAGTGCTGAACCCGTTCCGCGAGGC
CGTGGTGCAGCAGGTCGCTCGGGCGATGCAGTTCTTTTTTGGCGCCAGCCAGTACAACGCGGTGGATTATGTGGTTTTGG
CCGGCGGCACCTCGGCAATTCCCGGGCTTGTTGACATGGTAGAGGAAAAAACAGGCACGCCCACGCTGGTAGCCAATCCG
TTCGCGGATATGGCTGTTAGCTCGCGGGTTAATGCGTCGGCGTTGAGTAACGATGCTCCCTCGTTGATGATCGCGTGTGG
TCTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

51.412

100

0.514
  comM Acinetobacter nosocomialis M2

51.412

100

0.514
  comM Acinetobacter baylyi ADP1

50.565

100

0.506
  pilM Legionella pneumophila strain ERS1305867

44.915

100

0.449