Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   MIH18_RS07720 Genome accession   NZ_CP092284
Coordinates   1672437..1674152 (+) Length   571 a.a.
NCBI ID   WP_249014253.1    Uniprot ID   -
Organism   Marinobacter sp. M3C     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1667437..1679152
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MIH18_RS07700 (MIH18_07695) - 1668234..1669208 (-) 975 WP_249014250.1 IS1595 family transposase -
  MIH18_RS07705 (MIH18_07700) - 1669329..1669673 (+) 345 WP_283164888.1 hypothetical protein -
  MIH18_RS07710 (MIH18_07705) - 1669737..1670816 (-) 1080 WP_249014251.1 glycosyltransferase family 4 protein -
  MIH18_RS07715 (MIH18_07710) tviB 1670932..1672194 (-) 1263 WP_249014252.1 Vi polysaccharide biosynthesis UDP-N-acetylglucosamine C-6 dehydrogenase TviB -
  MIH18_RS07720 (MIH18_07715) pilB 1672437..1674152 (+) 1716 WP_249014253.1 type IV-A pilus assembly ATPase PilB Machinery gene
  MIH18_RS07725 (MIH18_07720) pilC 1674155..1675372 (+) 1218 WP_249014254.1 type II secretion system F family protein Machinery gene
  MIH18_RS07730 (MIH18_07725) pilD 1675376..1676251 (+) 876 WP_249014255.1 A24 family peptidase Machinery gene
  MIH18_RS07735 (MIH18_07730) coaE 1676308..1676916 (+) 609 WP_249007803.1 dephospho-CoA kinase -
  MIH18_RS07740 (MIH18_07735) - 1676888..1677997 (+) 1110 WP_249014256.1 hypothetical protein -
  MIH18_RS07745 (MIH18_07740) tsaA 1678003..1678758 (+) 756 WP_249014257.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -
  MIH18_RS07750 (MIH18_07745) yacG 1678835..1679032 (+) 198 WP_249007800.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62478.67 Da        Isoelectric Point: 5.3445

>NTDB_id=656502 MIH18_RS07720 WP_249014253.1 1672437..1674152(+) (pilB) [Marinobacter sp. M3C]
MTINTSNITLTGLARRFVSDGLLEESTAKDAFFQASQNRIPLITYLTQHKLANSSALAFSAAMEFGISVLDLDAFLPEMM
PDKLVDEKLLRKHNALPLYKRGNRLFIAVSDPTNVQALDEIKFNTGLSTDAILVDDSKLRAAIEKYLASSESSMGDLADT
DLEGIETEGADGNEDDGAVVVAEVDDAPIVKYVNKMLLDAIRGGSSDVHFEPYEKTYRVRFRTDGILKEMSRPSIKLAPK
ISARIKIMAQLDISERRLPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDALGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTDERNISTAEDPAEINLEGVNQVNVNNKVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSCKQAGAIPKDVLLAE
GFSTEQIETGFTLYHPKGCDKCTNGYKGRVGIYEVVKVTEQLASMIMEEASSIKIAKAAQAEGFRTLRQSALLKVIEGVT
SLEEANRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=656502 MIH18_RS07720 WP_249014253.1 1672437..1674152(+) (pilB) [Marinobacter sp. M3C]
ATGACAATAAACACAAGTAACATCACCTTAACCGGCCTGGCACGGCGTTTCGTAAGCGACGGCTTGCTGGAAGAATCCAC
GGCCAAAGACGCCTTTTTTCAGGCTTCTCAGAACCGTATTCCGCTGATTACCTACCTAACTCAGCACAAGTTGGCAAACA
GCTCGGCGTTGGCGTTTTCGGCGGCCATGGAGTTTGGTATTTCAGTGCTGGACCTGGATGCGTTTTTGCCGGAGATGATG
CCCGACAAGCTGGTGGACGAAAAACTGCTGCGCAAGCACAACGCCCTGCCCCTGTATAAGCGTGGCAACCGGCTGTTTAT
TGCAGTGAGCGACCCCACCAACGTTCAGGCTCTGGACGAAATCAAGTTCAATACCGGCTTAAGCACCGATGCAATTCTGG
TGGACGATTCCAAGCTGCGCGCGGCTATCGAAAAGTATTTGGCGTCTAGTGAAAGCTCCATGGGCGACCTGGCCGATACA
GACCTTGAAGGTATCGAAACAGAAGGCGCGGATGGAAACGAAGACGACGGTGCAGTGGTTGTTGCCGAAGTAGACGATGC
ACCCATCGTAAAGTATGTGAACAAAATGCTGCTAGATGCGATTCGCGGGGGTTCTTCAGACGTTCACTTTGAACCTTACG
AGAAAACCTACCGCGTACGCTTTCGCACTGACGGCATTCTAAAAGAAATGTCGCGACCCTCTATAAAGCTGGCACCGAAA
ATATCCGCGCGAATAAAGATCATGGCGCAGCTGGACATTTCCGAGCGACGTTTGCCGCAAGACGGCCGCATAAAAATGAG
GCTGTCCAAAACCAAGTCCATCGACTTTCGGGTGAACACCCTGCCCACGTTGTGGGGCGAGAAAATCGTGCTGCGTATTC
TGGACCCCAGCCAGGCAAAGCTGGGCATTGACGCCCTCGGCTATGAAGAAGACCAGAAGAAGCTGTATATGGACGCCCTG
GCGCAACCACAGGGCATGATTCTGGTGACGGGCCCCACCGGTTCCGGTAAAACCGTGTCGCTGTATACCGGCCTGAACAT
TCTGAACACCGACGAGCGCAATATTTCCACCGCGGAAGACCCGGCGGAAATTAACCTGGAAGGCGTGAATCAGGTCAACG
TGAACAACAAAGTGGGGCTTGGCTTCGCCGAAGCGCTACGGGCGTTTTTGCGGCAAGACCCTGACGTTATCATGGTGGGC
GAGATTCGAGACCTGGAAACCGCTAACATTGCTATCAAAGCGGCACAAACCGGTCACCTTGTGTTATCCACCCTGCACAC
CAACAGCGCGGCCGAAACCTTAACCCGTATGATGAACATGGGTGTGCCGGCGTTTAACATTGCCACGTCTGTTAGCCTGA
TTATTGCCCAGCGCCTGGGCCGGCGACTTTGCAGCTGCAAACAGGCTGGAGCCATCCCAAAAGACGTACTTTTAGCCGAA
GGGTTCTCAACTGAACAAATTGAAACAGGGTTCACGTTGTACCACCCGAAAGGCTGTGATAAATGCACTAATGGGTATAA
AGGCCGTGTCGGCATTTACGAGGTGGTGAAAGTAACCGAGCAGCTGGCCAGCATGATTATGGAAGAGGCCAGTTCTATCA
AGATTGCAAAAGCAGCTCAGGCTGAAGGCTTCCGAACCCTGCGCCAATCTGCCCTGTTAAAAGTGATTGAGGGTGTGACC
AGCCTTGAAGAAGCCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.46

98.949

0.559
  pilB Acinetobacter baylyi ADP1

54.625

100

0.548
  pilB Legionella pneumophila strain ERS1305867

52.85

100

0.536
  pilB Vibrio campbellii strain DS40M4

50.796

98.949

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

51.718

96.848

0.501
  pilB Vibrio cholerae strain A1552

54.159

90.543

0.49
  pilF Neisseria gonorrhoeae MS11

46.903

98.949

0.464
  pilB/pilB1 Synechocystis sp. PCC 6803

41.779

90.543

0.378