Detailed information NTG0652653

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: L5465_RS12385
• Genome accession: NZ_CP091596
• Coordinates: 2464476..2465534 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain Nord4-2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2459476..2470534

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L5465_RS12370 (L5465_12370)	-	2460582..2460914 (+)	333	WP_000169599.1	DUF7709 family protein	-
L5465_RS12375 (L5465_12375)	-	2460917..2461741 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
L5465_RS12380 (L5465_12380)	ponA	2461759..2464314 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
L5465_RS12385 (L5465_12385)	pilM	2464476..2465534 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
L5465_RS12390 (L5465_12390)	pilN	2465534..2466175 (+)	642	WP_000201221.1	PilN domain-containing protein	Machinery gene
L5465_RS12395 (L5465_12395)	pilO	2466172..2466912 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
L5465_RS12400 (L5465_12400)	pilP	2466923..2467450 (+)	528	WP_000695061.1	pilus assembly protein PilP	Machinery gene
L5465_RS12405 (L5465_12405)	pilQ	2467513..2469678 (+)	2166	WP_001017029.1	type IV pilus secretin PilQ family protein	Machinery gene
L5465_RS12410 (L5465_12410)	aroK	2469717..2470313 (+)	597	WP_313902375.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=652653 L5465_RS12385 WP_002014066.1 2464476..2465534(+) (pilM) [Acinetobacter baumannii strain Nord4-2]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=652653 L5465_RS12385 WP_002014066.1 2464476..2465534(+) (pilM) [Acinetobacter baumannii strain Nord4-2]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGACTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423