Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ85_RS10590 Genome accession   NZ_CP091522
Coordinates   2152194..2153873 (-) Length   559 a.a.
NCBI ID   WP_244739791.1    Uniprot ID   -
Organism   Neisseria sp. Dent CA1/247     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2147194..2158873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ85_RS10565 (LVJ85_10565) yacG 2148477..2148659 (-) 183 WP_085364438.1 DNA gyrase inhibitor YacG -
  LVJ85_RS10570 (LVJ85_10570) zapD 2148663..2149433 (-) 771 WP_244739788.1 cell division protein ZapD -
  LVJ85_RS10575 (LVJ85_10575) coaE 2149430..2150053 (-) 624 WP_244739789.1 dephospho-CoA kinase -
  LVJ85_RS10580 (LVJ85_10580) pilD 2150057..2150923 (-) 867 WP_370686917.1 A24 family peptidase Machinery gene
  LVJ85_RS10585 (LVJ85_10585) pilG 2150923..2152161 (-) 1239 WP_244739790.1 type II secretion system F family protein Machinery gene
  LVJ85_RS10590 (LVJ85_10590) pilF 2152194..2153873 (-) 1680 WP_244739791.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ85_RS10600 (LVJ85_10600) - 2154941..2155573 (+) 633 WP_244739792.1 isochorismatase family protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62333.03 Da        Isoelectric Point: 6.5114

>NTDB_id=652069 LVJ85_RS10590 WP_244739791.1 2152194..2153873(-) (pilF) [Neisseria sp. Dent CA1/247]
MSVGILRVLVQGQVIDNNQVERYQNVLKANKPILPLLFEEGIITPKALGELLARVFSYPLLDLTRYPRNNIVSDVLSEEQ
MLQNRCIPIFQRGRKVYFAVSDPTQIQNFQKIAFSSGMSVDLVVVRDDQLSTLLEWLGQRSTSILNEMSKEQEAAAPVQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKVPMERPPVPALKKAGFTDEDLAKNWTM
YRPVGCDSCRGKGYKGRAGVYEVMPITEEMQRVIMENGTEVDILNMAYKEGMVDLRRAGLLKVMQGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=652069 LVJ85_RS10590 WP_244739791.1 2152194..2153873(-) (pilF) [Neisseria sp. Dent CA1/247]
ATGAGCGTCGGTATATTAAGAGTATTGGTGCAGGGCCAAGTCATCGACAATAATCAGGTCGAACGGTATCAAAACGTATT
AAAAGCCAATAAACCCATTCTCCCTCTTTTGTTTGAAGAGGGTATTATCACACCTAAAGCATTAGGGGAACTGCTTGCCA
GAGTATTCAGCTATCCTCTGCTGGATTTAACCCGTTATCCCCGCAACAACATCGTTTCTGATGTTCTCTCGGAAGAGCAA
ATGCTGCAAAACCGCTGTATTCCGATTTTCCAAAGAGGCCGGAAAGTTTATTTTGCAGTTTCAGATCCCACCCAAATTCA
AAATTTCCAAAAAATAGCTTTTTCATCGGGTATGTCGGTCGATTTGGTGGTGGTTCGCGACGACCAACTGAGTACCTTGC
TTGAATGGTTGGGTCAACGTTCCACCTCGATTCTGAATGAGATGAGCAAGGAACAGGAAGCGGCCGCACCTGTTCAATCT
TTGTATATCGATAATGAAGAAGCAGAAGACGGGCCAATCCCCCGTTTCATTCATAAAACACTTTCCGATGCCTTGAATGC
AGGTGCTTCGGATATCCACTTTGAGTTTTACGAGCAGATGGCCCGCGTGCGTTTCCGTGTCGACGGCCAACTGCGCGAAG
TGGTGCAGCCGCCCGTAGCCGTGCGCGGCCAGCTTGCATCTCGGATTAAGGTGATGGCGCGTTTGGATATTTCTGAAAAA
CGCGTACCTCAAGACGGCCGTATTCAGATTGCCTTCCACAAACACGGCCGCCCGATCGACTTCCGTGTCAGCACACTGCC
GACATTGTTCGGCGAAAAAGTGGTGATGCGTATTCTGAATTCAGATGCGGGTTCGTTAAATATCGACCAATTGGGCTTTG
AACCGTTCCAAAAAGAAATGCTGCTGGAAGCCATCCACCGTCCTTACGGCATGGTGTTGGTAACGGGTCCCACCGGCTCG
GGTAAAACGGTGTCGCTTTATACCTGTTTGAACATCTTAAATACGGAAAATGTCAATATCGCCACGGCAGAAGACCCTGC
CGAGATTAATTTGCCCGGCATTAACCAAGTAAACGTAAACGATAAACAGGGTTTGACCTTTGCGGCGGCTCTTAAAGCAT
TCCTCCGTCAGGATCCGGATATCATCATGGTGGGTGAGATTCGTGATTTGGAAACTGCCGATATCGCCATTAAAGCCGCA
CAAACCGGCCACATGGTGTTTTCTACGCTGCACACCAATAACGCACCGGCAACGCTGTCGCGTATGCTGAATATGGGTGT
CGCACCGTTTAATATCGCCAGTTCTGTAAGCTTGATTATGGCGCAACGCCTGCTACGGCGGTTATGCCCGAATTGTAAGG
TTCCTATGGAGCGCCCTCCCGTTCCCGCATTGAAAAAAGCCGGTTTTACCGACGAAGATTTGGCGAAAAATTGGACCATG
TACCGCCCGGTGGGCTGCGATAGCTGCCGGGGTAAAGGATATAAAGGACGTGCGGGCGTTTACGAAGTTATGCCGATAAC
TGAAGAGATGCAACGCGTTATCATGGAAAACGGCACGGAAGTCGACATTCTCAATATGGCTTATAAAGAAGGCATGGTTG
ATTTGCGCCGTGCGGGCTTGTTGAAAGTCATGCAGGGCATCACTTCGTTGGAAGAAGTGTTGGCTCACACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.996

100

0.78
  pilB Acinetobacter baumannii D1279779

51.526

99.642

0.513
  pilB Acinetobacter baylyi ADP1

51.526

99.642

0.513
  pilB Legionella pneumophila strain ERS1305867

47.509

100

0.478
  pilB Vibrio cholerae strain A1552

45.745

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

100

0.456
  pilB Vibrio campbellii strain DS40M4

43.784

99.284

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.197

93.56

0.367
  pilF Thermus thermophilus HB27

38.258

94.454

0.361