Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   LVJ81_RS09520 Genome accession   NZ_CP091512
Coordinates   1951255..1952433 (+) Length   392 a.a.
NCBI ID   WP_019957390.1    Uniprot ID   -
Organism   Vitreoscilla stercoraria strain SAG 1488-6     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1946255..1957433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ81_RS09500 (LVJ81_09500) - 1947401..1948270 (+) 870 WP_019957395.1 NAD(P)H-hydrate dehydratase -
  LVJ81_RS13145 - 1948370..1948498 (+) 129 WP_019957394.1 hypothetical protein -
  LVJ81_RS09505 (LVJ81_09505) - 1948695..1949393 (+) 699 WP_019957393.1 CoA transferase subunit A -
  LVJ81_RS09510 (LVJ81_09510) - 1949407..1950036 (+) 630 WP_019957392.1 3-oxoacid CoA-transferase subunit B -
  LVJ81_RS09515 (LVJ81_09515) - 1950134..1950934 (+) 801 WP_019957391.1 3-hydroxybutyrate dehydrogenase -
  LVJ81_RS09520 (LVJ81_09520) pilU 1951255..1952433 (+) 1179 WP_019957390.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  LVJ81_RS09525 (LVJ81_09525) - 1952513..1953478 (-) 966 WP_019957389.1 histone deacetylase family protein -
  LVJ81_RS09530 (LVJ81_09530) - 1953714..1954382 (+) 669 WP_081619525.1 DsbA family oxidoreductase -
  LVJ81_RS09535 (LVJ81_09535) - 1954448..1954750 (-) 303 WP_019957387.1 hypothetical protein -
  LVJ81_RS09540 (LVJ81_09540) - 1955103..1957025 (-) 1923 WP_019957386.1 alpha/beta hydrolase -

Sequence

Protein


Download         Length: 392 a.a.        Molecular weight: 43645.31 Da        Isoelectric Point: 5.4471

>NTDB_id=651913 LVJ81_RS09520 WP_019957390.1 1951255..1952433(+) (pilU) [Vitreoscilla stercoraria strain SAG 1488-6]
MDRKQAYQYVCELLKLMLSKKSSDLFITVNYPPAMKIDGKMTKMDPQNLTATHTLALVQSIMTENQIKEFETTKECNFAI
SPNGIGRFRVNAFVQQGSAGMVLRVIPTELPTIDGLGVPEILKDVVMAKRGLCILVGGTGSGKSTTLAAMVDYRNENSHG
HIITIEDPVEFVHPHKNCVVMQREVGIDTDNWEIALKNTLRQAPDVILMGEIRDAETMEHAMAFAETGHLCLATLHANSA
NQAIDRIINFFPEERHQQLLMDLSLNLRGMVSQRLIPRKGGKGRAAAIEIMLNSPLISELIFKGETGEIKEIMKKSRELG
MQTFDQALFDLYMQDKISYEEALRNADSVNDLRLTIKLHGDQKPIDGVDENHDPLAALNLMNDADLAEALNS

Nucleotide


Download         Length: 1179 bp        

>NTDB_id=651913 LVJ81_RS09520 WP_019957390.1 1951255..1952433(+) (pilU) [Vitreoscilla stercoraria strain SAG 1488-6]
ATGGATCGCAAGCAAGCCTATCAATATGTTTGTGAATTGCTCAAACTCATGTTATCCAAAAAAAGCAGTGACTTATTCAT
TACGGTGAACTACCCTCCTGCTATGAAAATTGATGGCAAAATGACCAAAATGGATCCGCAAAATTTAACAGCGACGCATA
CTTTGGCATTGGTTCAATCCATCATGACGGAAAATCAAATCAAGGAATTTGAAACCACCAAAGAATGTAATTTTGCCATT
TCTCCCAATGGCATTGGTCGTTTTCGTGTCAATGCTTTTGTACAACAAGGTTCGGCAGGGATGGTATTGCGTGTGATTCC
AACCGAATTGCCGACCATTGATGGTTTGGGTGTGCCTGAAATTTTAAAAGATGTGGTAATGGCCAAACGTGGTTTGTGTA
TTTTGGTGGGCGGCACAGGCTCGGGCAAATCCACCACGCTCGCGGCGATGGTAGACTATCGCAATGAAAACTCACACGGT
CACATCATCACCATCGAAGACCCTGTGGAATTTGTACATCCACACAAAAACTGCGTGGTGATGCAGCGTGAAGTGGGCAT
TGATACCGACAATTGGGAAATTGCTTTGAAAAACACCTTGCGCCAAGCGCCTGATGTGATTTTGATGGGTGAGATTCGCG
ATGCGGAAACGATGGAACACGCGATGGCGTTTGCCGAAACGGGTCACTTGTGTTTGGCCACGTTGCATGCCAACAGCGCC
AACCAAGCGATTGACCGCATCATTAACTTCTTCCCAGAAGAACGCCACCAACAATTGCTGATGGACTTGTCTTTGAATTT
GCGTGGCATGGTTTCACAACGTTTGATTCCACGCAAAGGCGGTAAAGGACGTGCAGCTGCAATTGAAATCATGCTCAATA
GTCCATTGATTTCTGAATTGATTTTCAAAGGTGAAACCGGTGAAATCAAAGAAATCATGAAAAAATCGCGCGAATTGGGC
ATGCAAACCTTTGACCAAGCTTTGTTTGATTTGTATATGCAAGACAAAATTTCATACGAAGAGGCTTTGCGCAATGCCGA
CTCGGTCAACGATTTGCGCTTGACCATCAAATTGCATGGCGACCAAAAACCCATCGATGGTGTGGACGAAAACCACGACC
CATTGGCAGCATTGAATTTGATGAACGATGCTGATTTGGCAGAGGCTTTGAACTCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(20-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

61.671

88.52

0.546
  pilU Acinetobacter baylyi ADP1

56.863

91.071

0.518
  pilU Vibrio cholerae strain A1552

54.545

86.99

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.917

85.969

0.378
  pilT Pseudomonas aeruginosa PAK

43.323

85.969

0.372
  pilT Legionella pneumophila strain Lp02

42.643

84.949

0.362
  pilT Legionella pneumophila strain ERS1305867

42.643

84.949

0.362