Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ82_RS11540 Genome accession   NZ_CP091511
Coordinates   2233424..2235100 (+) Length   558 a.a.
NCBI ID   WP_058356446.1    Uniprot ID   -
Organism   Vitreoscilla massiliensis strain SN4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2228424..2240100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ82_RS11525 (LVJ82_11525) secA 2228707..2231382 (+) 2676 WP_058356449.1 preprotein translocase subunit SecA -
  LVJ82_RS11530 (LVJ82_11530) - 2231473..2231811 (-) 339 WP_058356448.1 helix-hairpin-helix domain-containing protein -
  LVJ82_RS11535 (LVJ82_11535) - 2232253..2233311 (+) 1059 WP_058356447.1 porin -
  LVJ82_RS11540 (LVJ82_11540) pilF 2233424..2235100 (+) 1677 WP_058356446.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ82_RS11545 (LVJ82_11545) pilG 2235126..2236364 (+) 1239 WP_058356445.1 type II secretion system F family protein Machinery gene
  LVJ82_RS11550 (LVJ82_11550) pilD 2236373..2237254 (+) 882 WP_108721531.1 A24 family peptidase Machinery gene
  LVJ82_RS11555 (LVJ82_11555) coaE 2237251..2237853 (+) 603 WP_058356443.1 dephospho-CoA kinase -
  LVJ82_RS11560 (LVJ82_11560) zapD 2237862..2238650 (+) 789 WP_058356442.1 cell division protein ZapD -
  LVJ82_RS11565 (LVJ82_11565) yacG 2238638..2238829 (+) 192 WP_058356441.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61252.88 Da        Isoelectric Point: 5.0296

>NTDB_id=651890 LVJ82_RS11540 WP_058356446.1 2233424..2235100(+) (pilF) [Vitreoscilla massiliensis strain SN4]
MDSGLLRILVQQQIISEEKAKGFIEKAVAENKSAVVFLLQEKIISAPQLAQLLAKLFSCPLFDLNFYNQDKLDFSALTED
LSTKYHAVPLLKRGRRLTVGVIDPSVLPLLQKATFSSGVSIDLVVVQADQLLGLLERIGASSTNMLDDFDADLIGGSQTM
AVAEEEQVEDGPIAKFVHKILLDALKAGASDIHFEFYEKTARVRFRVDGQLREVVNPPTQIREQIASRIKVMSALDISEK
RIPQDGRIKLKVSDTRAIDLRVSTLPTLFGEKIVMRILDSTAASLNIDQLGFEDFQKEMLLDAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNQPDVNISTAEDPAEINLPGINQVNVNDKQGLTFSAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLLRRLCVECKTPIERPPVEALVKLGFTEDMLAQDFEFC
GPVGCDRCRGSGYKGRLGVYEIMPITDEIQRVIMKNGTAVDIADIAYKEGMVDLRTAGLLKAMKGLTSLEEVSATTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=651890 LVJ82_RS11540 WP_058356446.1 2233424..2235100(+) (pilF) [Vitreoscilla massiliensis strain SN4]
ATGGACAGTGGCTTGTTACGAATATTGGTTCAACAGCAAATCATCAGCGAGGAAAAGGCCAAAGGTTTTATTGAAAAAGC
GGTAGCTGAAAACAAATCGGCTGTGGTGTTTTTATTGCAAGAAAAAATTATATCAGCTCCCCAATTGGCGCAGTTATTGG
CGAAATTATTCAGTTGTCCCTTGTTTGATTTGAATTTTTACAATCAAGATAAATTAGATTTTTCAGCCTTAACCGAAGAT
TTGAGTACCAAATACCATGCAGTGCCTTTATTGAAGCGGGGAAGAAGGTTAACTGTTGGTGTGATTGACCCATCGGTTTT
GCCGTTATTACAGAAAGCGACTTTTTCTTCCGGTGTTTCCATTGATTTGGTGGTGGTGCAGGCCGACCAATTATTGGGTT
TATTGGAACGTATTGGGGCTTCTTCTACCAATATGTTGGATGATTTTGATGCCGATTTAATTGGTGGCAGTCAAACCATG
GCGGTTGCAGAGGAAGAGCAGGTTGAAGATGGCCCTATCGCCAAGTTTGTGCATAAAATTTTGTTGGATGCCTTGAAGGC
AGGTGCTTCGGACATCCATTTTGAATTTTATGAGAAAACAGCACGGGTACGTTTTCGTGTGGATGGTCAATTGCGCGAGG
TGGTTAATCCCCCAACGCAAATTCGTGAGCAAATTGCATCACGGATTAAAGTGATGTCGGCATTGGATATCTCAGAAAAA
CGAATTCCTCAAGATGGTAGGATTAAATTAAAAGTCAGTGATACACGTGCCATTGACTTGCGTGTGAGTACATTGCCGAC
ACTCTTTGGTGAAAAAATTGTAATGCGTATTTTGGATTCTACGGCGGCATCTCTAAATATTGATCAGCTGGGGTTCGAAG
ATTTCCAAAAAGAAATGTTATTGGATGCCATCCATAGGCCTTATGGCATGGTGTTGGTAACGGGTCCTACGGGTTCAGGT
AAGACGGTGTCTTTGTATACATGTTTGAATATTTTGAATCAACCTGATGTGAATATTTCTACAGCCGAAGACCCTGCCGA
GATTAACTTACCTGGCATCAATCAAGTGAACGTGAATGATAAGCAAGGTTTGACATTTTCGGCCGCTTTAAAATCATTCT
TACGTCAAGACCCAGACATCATCATGGTGGGTGAGATACGTGACTTGGAGACCGCTGATATTGCGATTAAAGCCGCTCAA
ACAGGTCATATGGTGTTTTCAACTCTGCATACCAATAATGCTCCGGCGACTTTGTCGCGTATGTTAAACATGGGCGTGGC
GCCATTCAACATTGCTAGTTCAGTAAACTTGATTATGGCACAGCGACTGTTACGGCGCTTGTGTGTAGAATGTAAAACAC
CGATAGAGCGCCCACCGGTAGAAGCTTTGGTAAAACTAGGCTTTACTGAAGACATGTTGGCACAAGATTTCGAGTTTTGC
GGCCCAGTGGGGTGTGATCGTTGCCGTGGCAGTGGTTATAAAGGGCGTTTGGGTGTGTATGAAATCATGCCAATTACAGA
TGAAATTCAACGTGTTATTATGAAAAATGGCACGGCAGTAGACATTGCGGACATTGCGTATAAAGAGGGAATGGTGGATT
TGCGTACGGCGGGTTTGCTAAAAGCTATGAAGGGATTGACTTCATTAGAGGAAGTTTCAGCCACCACCAACGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(179-446)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

65.116

100

0.652
  pilB Acinetobacter baumannii D1279779

52.135

100

0.525
  pilB Acinetobacter baylyi ADP1

50.794

100

0.516
  pilB Legionella pneumophila strain ERS1305867

49.558

100

0.502
  pilB Vibrio cholerae strain A1552

45.276

100

0.455
  pilB Vibrio campbellii strain DS40M4

44.742

100

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

44.029

100

0.443
  pilF Thermus thermophilus HB27

40.075

95.699

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.691

93.369

0.38