Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ88_RS08485 Genome accession   NZ_CP091509
Coordinates   1839173..1840852 (-) Length   559 a.a.
NCBI ID   WP_085417695.1    Uniprot ID   -
Organism   Neisseria dumasiana strain LMG 30012     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1834173..1845852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ88_RS08455 (LVJ88_08455) mscL 1834834..1835277 (+) 444 WP_085417693.1 large conductance mechanosensitive channel protein MscL -
  LVJ88_RS08460 (LVJ88_08460) yacG 1835461..1835640 (-) 180 WP_082402963.1 DNA gyrase inhibitor YacG -
  LVJ88_RS08465 (LVJ88_08465) zapD 1835645..1836412 (-) 768 WP_054599204.1 cell division protein ZapD -
  LVJ88_RS08470 (LVJ88_08470) coaE 1836409..1837032 (-) 624 WP_085417694.1 dephospho-CoA kinase -
  LVJ88_RS08475 (LVJ88_08475) pilD 1837036..1837899 (-) 864 WP_054599206.1 A24 family peptidase Machinery gene
  LVJ88_RS08480 (LVJ88_08480) pilG 1837902..1839140 (-) 1239 WP_085356335.1 type II secretion system F family protein Machinery gene
  LVJ88_RS08485 (LVJ88_08485) pilF 1839173..1840852 (-) 1680 WP_085417695.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ88_RS08495 (LVJ88_08495) - 1841533..1842165 (+) 633 WP_054599209.1 isochorismatase family protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62204.74 Da        Isoelectric Point: 6.4272

>NTDB_id=651831 LVJ88_RS08485 WP_085417695.1 1839173..1840852(-) (pilF) [Neisseria dumasiana strain LMG 30012]
MSVGILRVLVQSQIIDNNQVERYQNVLKANKSIVPLLFEEGVITPKALGELLARVFSYPLLDLTHYPRSNILPDVLSEEQ
MLQNRCVPIFQRGRKVYFAVSDPTQIQNFQKIAFSSGVSVDLVVVRDDQLSTLLEWLGQRSTSILNEMSKEQEAAAPVQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIDQLGFEPFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSNCKTPMERPPVAALKKAGFTDEDLAKNWTM
YRPVGCDSCRGKGYKGRAGVYEVMPISEEMQRVIMENGTEVDILNMAYKEGMVDLRRAGLLKVMQGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=651831 LVJ88_RS08485 WP_085417695.1 1839173..1840852(-) (pilF) [Neisseria dumasiana strain LMG 30012]
ATGAGCGTCGGTATATTAAGAGTGTTGGTGCAAAGCCAAATCATTGACAATAATCAGGTCGAACGTTATCAAAACGTATT
AAAAGCAAATAAATCAATTGTTCCTCTGTTGTTTGAAGAGGGCGTAATCACACCTAAAGCATTAGGCGAGTTGCTGGCAA
GGGTTTTCAGCTACCCTCTCTTGGATTTAACCCATTATCCCCGCAGTAATATCCTCCCGGATGTGCTTTCCGAAGAACAA
ATGCTGCAAAACCGCTGTGTGCCGATTTTTCAAAGAGGCCGGAAAGTTTACTTCGCAGTATCCGATCCAACCCAAATTCA
GAATTTCCAAAAAATAGCCTTTTCATCAGGCGTATCGGTCGATTTGGTCGTGGTACGCGACGACCAGTTGAGCACTTTGC
TTGAATGGCTCGGCCAGCGTTCGACTTCGATTTTGAACGAAATGAGCAAAGAGCAGGAGGCTGCGGCTCCCGTTCAATCT
CTGTATATCGATAACGAAGAAGCAGAAGACGGGCCGATTCCCCGTTTTATTCACAAAACACTTTCCGATGCCTTAAATGC
GGGCGCTTCGGATATTCACTTCGAATTTTACGAGCAAATGGCGCGGGTTCGTTTCCGTGTGGACGGCCAGTTGAGAGAAG
TCGTTCAGCCGCCCGTAGCCGTCCGAGGCCAGCTTGCATCGCGTATCAAAGTGATGGCCCGTTTGGATATCTCGGAAAAA
AGAGTGCCTCAAGACGGCCGTATTCAAATCGCATTCCATAAGCACGGCCGCCCGATCGATTTCCGTGTCAGCACTTTGCC
CACTTTGTTCGGCGAAAAAGTGGTTATGCGTATTCTGAATTCAGATGCAGGGTCTTTGAATATCGACCAATTGGGCTTCG
AACCATTTCAAAAAGAATTGCTGCTGGAAGCCATCCACCGCCCCTACGGCATGGTATTGGTAACCGGCCCGACCGGTTCG
GGTAAAACGGTTTCACTCTATACCTGTTTGAACATCTTGAATACAGAGAATGTCAATATCGCTACTGCCGAAGACCCTGC
TGAGATTAACTTGCCCGGCATTAACCAGGTAAACGTAAACGATAAACAAGGCTTGACGTTTGCTGCCGCGCTGAAGGCGT
TTCTCCGTCAAGATCCGGATATCATCATGGTGGGTGAGATTCGGGATTTGGAAACTGCCGACATCGCCATTAAAGCTGCT
CAAACCGGCCATATGGTTTTCTCCACTTTGCATACTAATAACGCTCCTGCAACGCTGTCGCGTATGCTGAATATGGGTGT
TGCTCCGTTTAATATTGCCAGCTCCGTGAGCCTGATTATGGCTCAACGGCTTCTGCGCCGCTTGTGCTCGAATTGTAAAA
CGCCGATGGAACGCCCGCCTGTTGCGGCTTTGAAAAAAGCGGGTTTTACTGATGAAGATTTGGCAAAAAACTGGACTATG
TACCGTCCGGTAGGCTGCGACAGTTGCAGGGGGAAAGGCTATAAGGGGCGTGCCGGCGTATATGAGGTAATGCCGATAAG
CGAAGAAATGCAGCGCGTAATCATGGAAAACGGTACGGAAGTCGATATCTTAAATATGGCCTATAAAGAAGGCATGGTCG
ATTTGCGCCGCGCAGGATTGTTGAAAGTCATGCAAGGCATTACTTCGTTGGAAGAAGTGCTCGCTCATACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.891

100

0.789
  pilB Acinetobacter baumannii D1279779

52.065

99.642

0.519
  pilB Acinetobacter baylyi ADP1

51.706

99.642

0.515
  pilB Legionella pneumophila strain ERS1305867

47.331

100

0.476
  pilB Vibrio cholerae strain A1552

45.745

100

0.462
  pilB Vibrio parahaemolyticus RIMD 2210633

45.374

100

0.456
  pilB Vibrio campbellii strain DS40M4

43.964

99.284

0.436
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.388

93.56

0.369
  pilF Thermus thermophilus HB27

38.258

94.454

0.361