Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ83_RS02985 Genome accession   NZ_CP091508
Coordinates   658868..660547 (+) Length   559 a.a.
NCBI ID   WP_244786178.1    Uniprot ID   -
Organism   Uruburuella testudinis strain CCUG 63373m     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 653868..665547
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ83_RS02955 (LVJ83_02955) - 654307..655140 (-) 834 WP_244786168.1 transporter associated domain-containing protein -
  LVJ83_RS02960 (LVJ83_02960) ybeY 655143..655658 (-) 516 WP_244786170.1 rRNA maturation RNase YbeY -
  LVJ83_RS02965 (LVJ83_02965) - 655688..656266 (-) 579 WP_244786172.1 DUF4126 domain-containing protein -
  LVJ83_RS02970 (LVJ83_02970) - 656407..656892 (-) 486 WP_244786174.1 hypothetical protein -
  LVJ83_RS02975 (LVJ83_02975) - 657148..657828 (-) 681 WP_244786176.1 isochorismatase family protein -
  LVJ83_RS02985 (LVJ83_02985) pilF 658868..660547 (+) 1680 WP_244786178.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ83_RS02990 (LVJ83_02990) pilG 660564..661802 (+) 1239 WP_244786181.1 type II secretion system F family protein Machinery gene
  LVJ83_RS02995 (LVJ83_02995) pilD 661805..662665 (+) 861 WP_244787618.1 A24 family peptidase Machinery gene
  LVJ83_RS03000 (LVJ83_03000) coaE 662667..663281 (+) 615 WP_244786183.1 dephospho-CoA kinase -
  LVJ83_RS03005 (LVJ83_03005) zapD 663286..664053 (+) 768 WP_244787620.1 cell division protein ZapD -
  LVJ83_RS03010 (LVJ83_03010) yacG 664057..664248 (+) 192 WP_244787622.1 DNA gyrase inhibitor YacG -
  LVJ83_RS03015 (LVJ83_03015) - 664559..664720 (+) 162 WP_244786185.1 hypothetical protein -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61957.43 Da        Isoelectric Point: 6.6620

>NTDB_id=651773 LVJ83_RS02985 WP_244786178.1 658868..660547(+) (pilF) [Uruburuella testudinis strain CCUG 63373m]
MSVGLLRVLVQSQIVTHGQAERYQGALAAGQDIIPMLFDDGVTNPKALGELLARVFSYPLLDLRYYPRADVLMDVLSEEQ
MLQNRCVPIFRRGRKVYLAVSDPTQIHNFQKIAFSSGVSIDLVVVRDDQLSVLLEWLGQRSTTILKEISKEQQESHAAQA
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGALNIDQLGFEPFQKALLLEAISRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGVNQVNVNDKQGLTFAAALRAFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPACKTAMERPPQTALKKAGFSDADLSTEWPL
YRPVGCDSCRGKGYKGRVGVYEVMPVNEEMQRVILNNGTEVDIMNMAYQQGMVDLRRAGLMKVMQGVTSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=651773 LVJ83_RS02985 WP_244786178.1 658868..660547(+) (pilF) [Uruburuella testudinis strain CCUG 63373m]
ATGAGCGTCGGTTTATTAAGAGTGTTGGTACAAAGCCAGATTGTTACGCACGGCCAGGCGGAACGGTATCAGGGTGCGCT
GGCGGCCGGTCAGGATATTATTCCGATGCTGTTTGACGATGGCGTTACCAACCCTAAAGCATTGGGCGAATTGTTGGCAC
GGGTGTTCAGCTACCCGTTGTTGGACTTGCGTTATTATCCGCGCGCAGATGTTTTGATGGATGTGCTTTCTGAAGAGCAG
ATGCTGCAAAACCGCTGTGTGCCGATTTTCCGGCGCGGCCGTAAGGTATATTTGGCAGTGTCTGACCCTACGCAGATTCA
TAATTTTCAAAAGATTGCGTTTTCTTCGGGGGTGTCGATTGATTTGGTGGTAGTGCGGGATGACCAGCTCAGCGTATTGC
TGGAATGGCTCGGCCAGCGTTCGACGACGATTCTGAAAGAAATCAGCAAGGAGCAGCAGGAAAGCCATGCCGCGCAAGCG
CTGTATATCGACAATGAAGAAGCGGAAGACGGACCGATTCCGCGGTTTATCCATAAAACACTTTCCGATGCGCTGAATGC
GGGAGCTTCAGATATTCATTTTGAATTTTATGAGCAGATGGCGCGGGTGCGTTTCCGGGTAGACGGGCAGTTACGCGAGG
TGGTGCAGCCGCCGGTGGCGGTGCGCGGCCAGCTTGCTTCCCGTATTAAAGTGATGGCAAGGCTGGATATTTCCGAAAAG
CGCGTGCCGCAAGACGGCCGTATCCAAATCGCCTTTCACAAACACGGCCGACCGATTGATTTTCGGGTGAGTACGCTGCC
GACATTGTTCGGCGAAAAAGTGGTGATGCGGATTTTAAATTCGGATGCCGGTGCGCTCAATATCGATCAGCTCGGCTTCG
AGCCTTTTCAAAAGGCCTTGCTGCTGGAGGCCATCAGCCGCCCTTACGGTATGGTGCTGGTTACCGGCCCCACCGGCTCG
GGAAAAACCGTATCGCTTTATACCTGCCTCAATATTTTAAATACTGAAAATGTCAATATCGCAACCGCAGAAGACCCGGC
CGAAATTAATCTGCCCGGCGTGAATCAGGTTAATGTGAACGATAAGCAGGGGCTGACTTTTGCCGCCGCCTTGAGAGCGT
TTTTGCGCCAAGACCCGGATATCATCATGGTGGGGGAGATTCGCGATCTGGAAACCGCCGATATCGCCATTAAAGCCGCG
CAAACCGGCCATATGGTGTTCTCAACCCTGCACACCAATAACGCACCGGCAACCTTGTCGCGCATGCTCAATATGGGTGT
GGCCCCTTTTAATATCGCCAGCTCGGTCAGCCTGATTATGGCGCAGCGGCTGTTGCGGCGTTTGTGCCCGGCCTGCAAAA
CAGCCATGGAGCGCCCGCCGCAAACCGCTTTGAAAAAAGCCGGCTTTAGTGATGCGGATTTGAGCACAGAATGGCCGCTT
TACCGTCCGGTGGGCTGCGACAGCTGCCGCGGCAAGGGCTATAAAGGGCGGGTTGGCGTGTATGAGGTGATGCCGGTCAA
CGAAGAAATGCAGCGGGTGATTCTGAATAACGGCACCGAAGTGGATATTATGAATATGGCGTATCAGCAAGGCATGGTAG
ACTTGCGCCGTGCCGGTTTGATGAAAGTGATGCAGGGCGTAACTTCACTGGAAGAAGTGTTGGCGCATACCAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

76.744

100

0.767
  pilB Acinetobacter baylyi ADP1

51.699

100

0.517
  pilB Acinetobacter baumannii D1279779

50.893

100

0.51
  pilB Legionella pneumophila strain ERS1305867

46.619

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.213

100

0.456
  pilB Vibrio cholerae strain A1552

44.84

100

0.451
  pilB Vibrio campbellii strain DS40M4

43.95

100

0.442
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.313

93.739

0.369
  pilF Thermus thermophilus HB27

39.015

94.454

0.369