Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   LVJ78_RS11635 Genome accession   NZ_CP091507
Coordinates   2532648..2534330 (+) Length   560 a.a.
NCBI ID   WP_132953496.1    Uniprot ID   A0AAE9GWW3
Organism   Uruburuella suis strain 1258/02     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2527648..2539330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LVJ78_RS11610 (LVJ78_11610) asd 2527946..2529064 (-) 1119 WP_132953492.1 aspartate-semialdehyde dehydrogenase -
  LVJ78_RS11615 (LVJ78_11615) - 2529251..2529841 (+) 591 WP_165870901.1 RDD family protein -
  LVJ78_RS11620 (LVJ78_11620) - 2530051..2530551 (+) 501 WP_132953494.1 DUF4189 domain-containing protein -
  LVJ78_RS11625 (LVJ78_11625) - 2530844..2531497 (-) 654 WP_132953495.1 isochorismatase family protein -
  LVJ78_RS11635 (LVJ78_11635) pilF 2532648..2534330 (+) 1683 WP_132953496.1 type IV-A pilus assembly ATPase PilB Machinery gene
  LVJ78_RS11640 (LVJ78_11640) pilG 2534346..2535584 (+) 1239 WP_132953497.1 type II secretion system F family protein Machinery gene
  LVJ78_RS11645 (LVJ78_11645) pilD 2535586..2536452 (+) 867 WP_132953498.1 A24 family peptidase Machinery gene
  LVJ78_RS11650 (LVJ78_11650) coaE 2536449..2537072 (+) 624 WP_132953499.1 dephospho-CoA kinase -
  LVJ78_RS11655 (LVJ78_11655) zapD 2537069..2537830 (+) 762 WP_132953500.1 cell division protein ZapD -
  LVJ78_RS11660 (LVJ78_11660) yacG 2537835..2538023 (+) 189 WP_132953501.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 62483.11 Da        Isoelectric Point: 6.7751

>NTDB_id=651763 LVJ78_RS11635 WP_132953496.1 2532648..2534330(+) (pilF) [Uruburuella suis strain 1258/02]
MSVGLLRVLVQSQVIVGNQAERYQKALNEHKSIMPMLFEEGIISPRALGELLARMFSYPLLDLRHYPRANILPDILTEEQ
MLQNRCIPIFKRGRKVYLAVSDPTQIHNFQKIAFNSGASIDLVVVRDDQLSTLLEWLGQRSTTILQEISKEQQERAPQGG
VLYVDNEEAEDGPIARFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISE
KRIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIEQLGFEPFQKELLLEAIHRPYGMVLVTGPTG
SGKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALRSFLRQDPDIIMVGEIRDLETADIAIKA
SQTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVNLIMAQRLLRRLCPACKAPVERPPLPALKKAGFSDEDLAKEWT
LYRPVGCDNCRGKGYKGRAGVYEVMPVSEEMQRVIMNNGTEVDIMNMAYQEGMVDLRHAGLLKVMQGLTSLEEVLAHTND

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=651763 LVJ78_RS11635 WP_132953496.1 2532648..2534330(+) (pilF) [Uruburuella suis strain 1258/02]
ATGAGTGTCGGTTTATTGAGAGTATTGGTTCAAAGTCAGGTTATTGTGGGCAACCAGGCAGAACGCTACCAAAAAGCGCT
AAACGAACATAAAAGCATCATGCCCATGTTGTTTGAAGAAGGCATCATCTCGCCCCGCGCTTTGGGCGAATTGCTGGCGC
GCATGTTCAGCTATCCTTTGCTGGATTTGCGCCATTATCCGCGCGCCAATATTTTGCCCGACATTCTCACCGAAGAACAA
ATGCTGCAAAACCGCTGCATTCCGATTTTCAAGCGCGGCCGTAAAGTGTATTTGGCGGTGTCGGATCCCACTCAAATTCA
TAATTTCCAGAAAATTGCATTTAATTCCGGCGCATCCATCGATTTGGTGGTGGTGCGCGACGATCAATTAAGCACGCTTT
TGGAATGGCTGGGGCAGCGCTCCACCACCATTTTGCAGGAAATCAGCAAAGAGCAGCAGGAACGCGCCCCCCAAGGCGGT
GTGCTGTATGTTGATAATGAAGAAGCGGAAGACGGCCCGATCGCCCGCTTTATCCACAAAACCCTTTCCGATGCGCTCAA
TGCGGGCGCATCGGATATCCATTTTGAATTTTACGAGCAAATGGCGCGCGTGCGCTTCCGTGTGGACGGGCAATTGCGCG
AAGTGGTGCAGCCGCCTGTGGCCGTGCGCGGGCAGCTGGCTTCGCGCATCAAGGTAATGGCGCGCCTCGATATTTCGGAA
AAACGCATTCCGCAAGACGGCCGTATTCAAATCGCGTTCCACAAACACGGCCGCCCGATTGATTTCCGCGTCAGCACCTT
GCCGACTTTGTTTGGCGAAAAAGTGGTGATGCGTATCCTCAATTCAGATGCGGGCTCGCTCAATATCGAGCAATTGGGCT
TTGAGCCTTTTCAGAAAGAATTGCTGCTGGAAGCCATCCACCGCCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGC
TCGGGCAAAACCGTTTCGCTCTATACCTGCCTGAATATTCTCAACACGGAAAACGTGAATATCGCCACCGCGGAAGACCC
GGCCGAGATCAACCTGCCCGGCATCAACCAAGTAAACGTCAACGACAAACAAGGGCTCACCTTTGCTGCGGCCTTGCGCT
CCTTTTTGCGCCAAGATCCCGACATTATCATGGTGGGCGAAATCCGCGATTTGGAAACGGCCGACATCGCCATCAAAGCT
TCCCAAACCGGCCACATGGTGTTTTCAACGCTCCACACCAATAATGCGCCGGCCACTTTATCGCGCCTGCTCAACATGGG
TGTGGCGCCTTTCAACATTGCCAGCTCGGTAAACCTGATTATGGCCCAGCGCTTATTGCGCCGATTATGCCCGGCCTGCA
AAGCACCGGTCGAGCGCCCGCCGCTGCCCGCTTTGAAAAAAGCCGGCTTTTCCGATGAAGACTTGGCCAAAGAATGGACG
CTCTACCGCCCCGTGGGCTGTGACAATTGTCGCGGCAAAGGTTATAAAGGCCGCGCCGGGGTGTATGAAGTGATGCCGGT
TTCAGAAGAAATGCAACGCGTGATTATGAATAACGGCACCGAAGTGGACATTATGAATATGGCTTATCAAGAAGGCATGG
TAGACTTGCGCCATGCCGGCTTGCTAAAGGTAATGCAGGGGCTTACCTCGCTGGAAGAAGTGCTGGCGCACACCAATGAT
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-139)

Type II/IV secretion system protein (T2SSE)

(180-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

76.786

100

0.768
  pilB Acinetobacter baumannii D1279779

50.175

100

0.511
  pilB Acinetobacter baylyi ADP1

51.259

99.286

0.509
  pilB Legionella pneumophila strain ERS1305867

47.865

100

0.48
  pilB Vibrio cholerae strain A1552

45.552

100

0.457
  pilB Vibrio parahaemolyticus RIMD 2210633

45.035

100

0.454
  pilB Vibrio campbellii strain DS40M4

43.594

100

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.695

93.571

0.371
  pilF Thermus thermophilus HB27

39.057

94.643

0.37