Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   L3474_RS09540 Genome accession   NZ_CP091450
Coordinates   1862404..1863420 (-) Length   338 a.a.
NCBI ID   WP_013193332.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain 3641/15     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1857404..1868420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3474_RS09495 - 1857488..1857619 (-) 132 WP_000768904.1 hypothetical protein -
  L3474_RS09500 (L3474_09430) - 1857620..1858810 (-) 1191 WP_000167766.1 acetate kinase -
  L3474_RS09505 (L3474_09435) comYH 1858861..1859814 (-) 954 WP_000345119.1 class I SAM-dependent methyltransferase Machinery gene
  L3474_RS09510 (L3474_09440) - 1859875..1860462 (-) 588 WP_000679774.1 class I SAM-dependent methyltransferase -
  L3474_RS09515 (L3474_09445) comGG/cglG 1860599..1861012 (-) 414 WP_000265630.1 competence type IV pilus minor pilin ComGG Machinery gene
  L3474_RS09520 (L3474_09450) comGF/cglF 1860990..1861451 (-) 462 WP_000250545.1 competence type IV pilus minor pilin ComGF Machinery gene
  L3474_RS09525 (L3474_09455) comGE/cglE 1861414..1861716 (-) 303 WP_000413380.1 competence type IV pilus minor pilin ComGE Machinery gene
  L3474_RS09530 (L3474_09460) comGD/cglD 1861679..1862083 (-) 405 WP_000588005.1 competence type IV pilus minor pilin ComGD Machinery gene
  L3474_RS09535 (L3474_09465) comGC/cglC 1862076..1862402 (-) 327 WP_000738650.1 comG operon protein ComGC Machinery gene
  L3474_RS09540 (L3474_09470) comGB/cglB 1862404..1863420 (-) 1017 WP_013193332.1 competence type IV pilus assembly protein ComGB Machinery gene
  L3474_RS09545 (L3474_09475) comGA/cglA/cilD 1863368..1864309 (-) 942 WP_000249567.1 competence type IV pilus ATPase ComGA Machinery gene
  L3474_RS09550 (L3474_09480) - 1864385..1864750 (-) 366 WP_000286415.1 DUF1033 family protein -
  L3474_RS09555 (L3474_09485) - 1864901..1865959 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  L3474_RS09560 (L3474_09490) nagA 1866122..1867273 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38524.60 Da        Isoelectric Point: 9.6162

>NTDB_id=651285 L3474_RS09540 WP_013193332.1 1862404..1863420(-) (comGB/cglB) [Streptococcus pneumoniae strain 3641/15]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSALLDKQCVTQMRVGLSQGKSFSEMMESLGCSSAI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=651285 L3474_RS09540 WP_013193332.1 1862404..1863420(-) (comGB/cglB) [Streptococcus pneumoniae strain 3641/15]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACCATCTCCTTTTTAGATAGGAGTGCTTTGTTGGACAAGCAGTGTG
TAACCCAGATGCGTGTGGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTGCTATT
GTCACTCAGTTATCCCTAGCTGAAGTTCATGGCAATCTCCACCTGAGTTTGGGAAAGATAGAAGAATATCTGGACAATCT
GGCCAAGGTCAAGAAAAAATTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGTTTTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTTTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae D39

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae R6

99.112

100

0.991
  comGB/cglB Streptococcus pneumoniae TIGR4

99.112

100

0.991
  comGB/cglB Streptococcus mitis SK321

94.97

100

0.95
  comGB/cglB Streptococcus mitis NCTC 12261

94.379

100

0.944
  comYB Streptococcus gordonii str. Challis substr. CH1

70.833

99.408

0.704
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503