Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L3Q70_RS02625 Genome accession   NZ_CP091442
Coordinates   549363..551039 (-) Length   558 a.a.
NCBI ID   WP_062570063.1    Uniprot ID   -
Organism   Pseudoalteromonas sp. CF6-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 544363..556039
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L3Q70_RS02595 (L3Q70_02595) - 544400..545308 (+) 909 WP_237114806.1 cation diffusion facilitator family transporter -
  L3Q70_RS02600 (L3Q70_02600) - 545287..546228 (-) 942 WP_237114807.1 alpha/beta fold hydrolase -
  L3Q70_RS02605 (L3Q70_02605) - 546231..547613 (-) 1383 WP_083515341.1 ATP-binding protein -
  L3Q70_RS02610 (L3Q70_02610) - 547615..548304 (-) 690 WP_062570060.1 response regulator transcription factor -
  L3Q70_RS02615 (L3Q70_02615) - 548476..549006 (+) 531 WP_062570061.1 Spy/CpxP family protein refolding chaperone -
  L3Q70_RS02620 (L3Q70_02620) yacG 549056..549289 (-) 234 WP_062570062.1 DNA gyrase inhibitor YacG -
  L3Q70_RS02625 (L3Q70_02625) pilB 549363..551039 (-) 1677 WP_062570063.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L3Q70_RS02630 (L3Q70_02630) coaE 551214..551819 (-) 606 WP_237114808.1 dephospho-CoA kinase -
  L3Q70_RS02635 (L3Q70_02635) pilD 551822..552718 (-) 897 WP_276574952.1 A24 family peptidase Machinery gene
  L3Q70_RS02640 (L3Q70_02640) pilC 553097..554326 (-) 1230 WP_237114809.1 type II secretion system F family protein Machinery gene
  L3Q70_RS02645 (L3Q70_02645) - 554556..554975 (-) 420 WP_237114810.1 pilin -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 62311.57 Da        Isoelectric Point: 5.2662

>NTDB_id=651159 L3Q70_RS02625 WP_062570063.1 549363..551039(-) (pilB) [Pseudoalteromonas sp. CF6-2]
MNINSPLLRKFITLGRIDAETVKLKQREYNSTAELISKSCQIDGHELAEQCTDLFRVPYFDIKDFDVSLISEELIKEKLI
REHHILPLIKKGRKLYIAASDPTDYGAFENFEFSTGLSCEVVVVDYNQLENKIEQLLDATGGLHLSEDEFKELGDLETEK
PKDISGSDEDKDDAPIIVYINKILMDAIKKGASDLHFEPYEHRYRVRFRIDGILHEMASPPNSLASRLSARIKVMSRLDI
AEKRKPQDGRIKLKITERKSIDFRVSTLPTLWGEKIVMRILDSSSAMLGIDVLGYEPEQKKLYMDALEQPQGMILVTGPT
GSGKTVSLYTGLNILNQPERNISTAEDPVEINLEGINQVQINPKADMTFANALKAFLRQDPDVVMVGEIRDLETAEISIK
AAQTGHLVLSTLHTNSAPETLTRLLNMGVPAYNVASSISLIIAQRLARRLCPKCKTPEKLPDEELIRQGFDKDTLSEIQL
FAPKGCDSCTEGYKGRVGIYEVMQITPEIAQIIMRGGNSLEIAEVALKSGFNNLRLSGLKKAADGVTSLAEVNRVTSF

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=651159 L3Q70_RS02625 WP_062570063.1 549363..551039(-) (pilB) [Pseudoalteromonas sp. CF6-2]
ATGAATATTAACTCACCGTTACTTAGAAAATTTATTACTCTTGGCCGTATTGACGCAGAAACAGTAAAACTCAAGCAGCG
TGAATATAACTCTACGGCTGAGCTTATTTCTAAAAGCTGCCAGATTGATGGCCATGAGCTTGCGGAGCAGTGCACCGATC
TTTTTCGCGTTCCTTATTTTGATATTAAAGATTTTGATGTCTCGCTTATCAGTGAAGAGCTGATAAAAGAAAAGCTAATT
CGCGAACATCACATTCTACCGCTCATTAAAAAGGGCCGTAAACTTTATATTGCAGCCTCTGACCCTACTGACTACGGCGC
ATTTGAAAACTTTGAATTTAGTACAGGGTTATCATGTGAAGTGGTAGTGGTTGATTACAACCAGTTAGAAAATAAAATAG
AACAACTGCTGGATGCAACAGGTGGTCTGCACTTAAGCGAAGATGAATTTAAAGAACTGGGCGATCTAGAAACTGAAAAA
CCTAAAGATATTTCAGGCAGTGATGAAGACAAAGACGATGCCCCTATCATCGTCTACATCAATAAAATATTGATGGACGC
CATTAAAAAAGGCGCATCCGATTTACACTTTGAACCTTATGAACACCGCTATCGAGTGCGCTTTCGTATTGATGGCATCT
TACATGAAATGGCCAGTCCGCCGAATAGCTTAGCAAGTCGTTTATCAGCACGTATCAAAGTGATGTCTCGCTTAGATATT
GCAGAAAAACGTAAACCTCAAGATGGTCGCATTAAACTAAAAATAACAGAGCGAAAAAGTATCGATTTTCGTGTCAGTAC
CTTACCAACTCTTTGGGGCGAGAAAATCGTTATGCGTATTCTCGACTCATCAAGTGCCATGCTCGGTATTGATGTACTCG
GTTATGAACCAGAGCAGAAAAAACTCTACATGGATGCCCTTGAGCAACCACAAGGTATGATATTGGTAACCGGCCCAACG
GGTTCTGGTAAAACCGTATCGCTTTATACTGGTTTAAATATTCTCAACCAGCCTGAGCGCAATATCAGTACCGCCGAAGA
TCCCGTTGAGATCAATCTTGAAGGCATTAACCAAGTGCAAATCAATCCAAAAGCAGATATGACCTTTGCCAATGCCTTGA
AAGCCTTCTTACGACAAGATCCCGATGTGGTTATGGTGGGTGAGATCCGTGACCTTGAAACTGCTGAAATCTCAATCAAA
GCAGCACAAACAGGCCACTTGGTTTTATCTACTTTGCATACTAACTCGGCACCAGAAACCTTAACCCGTTTATTAAATAT
GGGTGTGCCTGCGTATAACGTAGCAAGCTCAATTAGTCTTATCATTGCACAGCGTCTAGCGCGTCGTTTATGCCCTAAAT
GCAAGACCCCAGAAAAGCTCCCCGATGAAGAGCTTATTCGCCAAGGTTTTGATAAAGACACATTAAGTGAAATTCAATTA
TTTGCACCTAAAGGGTGCGACAGTTGTACCGAAGGCTATAAAGGCCGTGTTGGTATTTATGAAGTTATGCAGATCACACC
TGAAATAGCGCAAATTATTATGCGTGGTGGTAATTCGCTTGAAATTGCAGAGGTGGCGCTTAAATCTGGCTTTAATAACT
TAAGACTGTCGGGGTTAAAAAAAGCAGCAGATGGTGTTACCTCTTTGGCTGAAGTAAATCGTGTGACGAGTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-449)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.863

100

0.572
  pilB Acinetobacter baylyi ADP1

56.071

100

0.563
  pilB Legionella pneumophila strain ERS1305867

54.228

97.491

0.529
  pilB Vibrio cholerae strain A1552

54.198

93.907

0.509
  pilB Vibrio campbellii strain DS40M4

52.425

96.057

0.504
  pilB Vibrio parahaemolyticus RIMD 2210633

53.757

93.011

0.5
  pilF Neisseria gonorrhoeae MS11

50

93.19

0.466
  pilB/pilB1 Synechocystis sp. PCC 6803

37.641

100

0.418
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.104

100

0.382
  pilF Thermus thermophilus HB27

37.316

97.491

0.364