Detailed information NTG0647735

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: L1704_RS08810
• Genome accession: NZ_CP091107
• Coordinates: 1740226..1740873 (-)
• Length: 215 a.a.
• NCBI ID: WP_058225668.1
• Uniprot ID: -
• Organism: Lactococcus lactis subsp. lactis strain UC109
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1735226..1745873

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
L1704_RS08780 (L1704_08780)	atpF	1735287..1735793 (-)	507	WP_058221761.1	F0F1 ATP synthase subunit B	-
L1704_RS08785 (L1704_08785)	atpB	1735808..1736521 (-)	714	WP_004255255.1	F0F1 ATP synthase subunit A	-
L1704_RS08790 (L1704_08790)	-	1736566..1736781 (-)	216	WP_004255250.1	F0F1 ATP synthase subunit C	-
L1704_RS08795 (L1704_08795)	-	1736965..1737741 (-)	777	WP_004255244.1	alpha/beta hydrolase family protein	-
L1704_RS08800 (L1704_08800)	comEC	1738026..1739585 (-)	1560	WP_345893333.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
L1704_RS08805 (L1704_08805)	comEC	1739603..1740136 (-)	534	WP_235589824.1	ComEC/Rec2 family competence protein	Machinery gene
L1704_RS08810 (L1704_08810)	comEA	1740226..1740873 (-)	648	WP_058225668.1	ComEA family DNA-binding protein	Machinery gene
L1704_RS08815 (L1704_08815)	-	1740933..1742294 (-)	1362	WP_021214597.1	ABC transporter permease	-
L1704_RS08820 (L1704_08820)	-	1742291..1743223 (-)	933	WP_004255238.1	ABC transporter ATP-binding protein	-
L1704_RS08825 (L1704_08825)	-	1743328..1743726 (-)	399	WP_004255236.1	hypothetical protein	-
L1704_RS08830 (L1704_08830)	-	1743830..1744393 (-)	564	WP_004255233.1	GNAT family N-acetyltransferase	-
L1704_RS08835 (L1704_08835)	-	1744566..1745744 (-)	1179	WP_021214596.1	SLC13 family permease	-

Sequence

Protein

• Length: 215 a.a.
• Molecular weight: 23650.89 Da
• Isoelectric Point: 5.0062

>NTDB_id=647735 L1704_RS08810 WP_058225668.1 1740226..1740873(-) (comEA) [Lactococcus lactis subsp. lactis strain UC109]
MDKILEKVKEYWKMIVLVVCGLIAGGIFYVLTNGQKPTTNLSVENLSSVSRQSSVSKFSEPNEKSVSKIMVDLKGAVTKP
NVYQISSDERLVDLIKEAGGFTDQADQKSINLSAKLKDEEVIYVPKIGENSSTENTDSSANSVTSQVSKTTEKININQAD
LTELQKLTGVGQKKAQDIIDFRTKIGDFKSLEDLGKVSGFGDKTLEKLKDELCFE

Nucleotide

• Length: 648 bp

>NTDB_id=647735 L1704_RS08810 WP_058225668.1 1740226..1740873(-) (comEA) [Lactococcus lactis subsp. lactis strain UC109]
ATGGATAAGATTTTAGAAAAAGTAAAAGAATATTGGAAAATGATTGTTTTAGTTGTTTGTGGGCTCATTGCTGGTGGGAT
TTTTTACGTTTTAACCAACGGTCAAAAGCCAACTACAAATCTGTCAGTAGAAAATTTAAGTTCTGTCAGCAGGCAAAGCT
CTGTCAGTAAATTTAGTGAACCTAATGAAAAATCTGTCAGTAAAATTATGGTTGATTTAAAAGGTGCGGTAACAAAGCCT
AATGTCTATCAAATTTCGTCAGATGAACGTCTAGTTGATTTAATTAAAGAAGCTGGTGGTTTTACTGACCAAGCTGACCA
GAAATCAATTAATCTGTCAGCAAAATTAAAAGATGAAGAAGTAATTTATGTCCCAAAAATTGGTGAAAATTCAAGCACAG
AAAATACTGACAGCTCTGCTAATTCGGTCACTAGTCAAGTCTCAAAAACCACTGAAAAAATAAATATCAATCAAGCAGAT
TTGACTGAACTACAAAAATTAACAGGTGTTGGTCAGAAAAAAGCTCAAGACATCATCGATTTTCGGACGAAAATTGGTGA
TTTCAAATCACTTGAAGACTTGGGAAAAGTTTCTGGTTTTGGAGATAAAACACTAGAAAAATTGAAAGATGAGTTGTGTT
TTGAATAA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (151-212)

• SLBB domain (SLBB) (70-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Lactococcus lactis subsp. cremoris KW2	75.229	100	0.763
comEA/celA/cilE	Streptococcus mitis SK321	44.444	100	0.447
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	43.119	100	0.437
comEA/celA/cilE	Streptococcus pneumoniae Rx1	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae D39	41.096	100	0.419
comEA/celA/cilE	Streptococcus pneumoniae R6	41.096	100	0.419
comEA/celA/cilE	Streptococcus mitis NCTC 12261	41.284	100	0.419
comEA	Latilactobacillus sakei subsp. sakei 23K	36.564	100	0.386
comEA	Staphylococcus aureus MW2	37.089	99.07	0.367