Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L0B52_RS04970 Genome accession   NZ_CP090975
Coordinates   1068466..1070127 (-) Length   553 a.a.
NCBI ID   WP_409202322.1    Uniprot ID   -
Organism   Suttonella sp. R2A3     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1063466..1075127
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0B52_RS04950 (L0B52_04950) - 1064506..1065771 (+) 1266 WP_235063641.1 acetyl-CoA C-acyltransferase -
  L0B52_RS04955 (L0B52_04955) coaE 1065772..1066371 (-) 600 WP_235063642.1 dephospho-CoA kinase -
  L0B52_RS04960 (L0B52_04960) pilD 1066384..1067235 (-) 852 WP_235063643.1 A24 family peptidase Machinery gene
  L0B52_RS04965 (L0B52_04965) pilC 1067235..1068389 (-) 1155 WP_235063644.1 type II secretion system F family protein Machinery gene
  L0B52_RS04970 (L0B52_04970) pilB 1068466..1070127 (-) 1662 WP_409202322.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L0B52_RS04975 (L0B52_04975) - 1070163..1070738 (-) 576 WP_235063646.1 aminodeoxychorismate/anthranilate synthase component II -
  L0B52_RS04980 (L0B52_04980) recO 1070768..1071430 (+) 663 WP_235063647.1 DNA repair protein RecO -
  L0B52_RS04985 (L0B52_04985) erpA 1071427..1071777 (-) 351 WP_235063648.1 iron-sulfur cluster insertion protein ErpA -
  L0B52_RS04990 (L0B52_04990) - 1071850..1073139 (-) 1290 WP_235063649.1 DUF2254 domain-containing protein -
  L0B52_RS04995 (L0B52_04995) ung 1073208..1073897 (+) 690 WP_235063650.1 uracil-DNA glycosylase -

Sequence

Protein


Download         Length: 553 a.a.        Molecular weight: 61719.41 Da        Isoelectric Point: 5.0140

>NTDB_id=646835 L0B52_RS04970 WP_409202322.1 1068466..1070127(-) (pilB) [Suttonella sp. R2A3]
MVQHKICTPEQIRESQVSAQKKKANFLQQLISDGLLDANRLSEINRDVYNLPQVMLREMDIRRELTAQFKETAVRKHMAL
PVYLNSGRLFLATFDPANRMMLDEFKYQGNFTSVEPVVANMDVIEALIEEHYAGVGGMDRMFDDEEQDVDIEAISDALGA
LDGEEEAPVVRFVTGMLLDAIRTGASDLHFEPYEQTYRVRFRRDGVLQEVAAPPPSIANRITARLKVMADLDIAEKRVPQ
DGRIKLQVSETKAIDFRVSSLPTLYGEKIVLRILDSSAAKLDIDVLGFEPEQKQHYLDAINKPQGLVLVTGPTGSGKTVS
LYTGLNLLNKPTVNISTAEDPVEINLPGINQVNVNTKTGLDFAAALKAFLRQDPDIIMVGEIRDITTGEIAVKAAQTGHL
VLSTLHTNDVPQTIARLVNIGIPSYNIAASVNLIMAQRLARRLCSYCKVRDRKHHHDELIALGFEEEEVDDLRIYTAKGC
ERCSYQGYRGRAGIYQVVPISDELAELILHNASASDIEEQCQKEGHWDLRQSALNKVKQGITSIEEILRVTSD

Nucleotide


Download         Length: 1662 bp        

>NTDB_id=646835 L0B52_RS04970 WP_409202322.1 1068466..1070127(-) (pilB) [Suttonella sp. R2A3]
ATGGTGCAGCATAAAATCTGTACGCCTGAGCAAATTCGCGAATCGCAAGTCAGTGCACAAAAGAAAAAAGCGAACTTTTT
GCAACAGCTGATCAGCGATGGTTTGCTCGATGCAAATCGCCTGTCGGAAATTAACCGCGATGTGTATAACTTGCCGCAGG
TGATGTTGCGTGAAATGGATATTCGCCGGGAACTGACTGCGCAATTTAAAGAAACTGCGGTGCGTAAACACATGGCGCTG
CCGGTCTATCTTAATAGTGGACGGCTGTTTTTAGCCACGTTTGATCCGGCAAACCGCATGATGCTCGATGAGTTTAAGTA
TCAGGGTAATTTTACGTCTGTCGAGCCAGTGGTGGCGAATATGGACGTGATTGAGGCGCTGATTGAAGAGCATTATGCCG
GTGTCGGTGGTATGGATCGGATGTTCGATGATGAAGAACAGGACGTCGATATCGAGGCAATCAGCGATGCTTTGGGCGCG
CTTGATGGCGAAGAAGAAGCACCAGTGGTGCGTTTTGTGACCGGTATGCTGCTTGATGCCATCCGCACTGGGGCGTCAGA
TTTACACTTTGAGCCGTATGAACAAACCTACCGAGTGCGCTTTCGTCGCGATGGGGTGCTGCAAGAAGTTGCTGCGCCGC
CGCCGAGTATTGCCAACCGGATTACTGCACGTTTGAAGGTCATGGCGGATTTGGATATCGCCGAAAAACGCGTGCCGCAA
GACGGGCGCATTAAGCTGCAAGTTTCTGAAACCAAAGCGATTGATTTTCGTGTCAGCTCGCTGCCAACGCTCTATGGCGA
AAAAATCGTTCTGCGTATTCTCGATAGCTCGGCAGCGAAGCTCGATATCGATGTGCTCGGCTTTGAACCGGAGCAAAAAC
AACACTATCTTGACGCGATCAATAAACCGCAAGGGTTGGTGTTGGTTACCGGGCCTACCGGGTCGGGTAAAACGGTGTCA
CTCTATACGGGCTTAAATTTACTCAACAAACCCACGGTCAATATTTCAACTGCTGAAGATCCGGTGGAAATCAACTTGCC
CGGGATTAATCAGGTCAACGTCAACACCAAAACCGGGTTGGATTTTGCGGCTGCGCTGAAAGCATTTTTGCGTCAGGATC
CCGATATCATCATGGTTGGGGAGATTCGTGATATCACCACCGGCGAGATCGCGGTCAAAGCGGCGCAAACCGGGCACTTG
GTGCTATCCACGCTGCATACCAATGACGTGCCGCAGACGATTGCGCGCTTGGTTAATATTGGCATTCCTTCGTATAATAT
TGCCGCCAGCGTCAATTTGATTATGGCGCAGCGTTTGGCGCGGCGTTTATGTTCCTATTGTAAAGTACGCGACCGTAAGC
ACCATCATGACGAGCTGATTGCGCTCGGTTTCGAGGAAGAAGAGGTCGATGATTTGCGCATCTATACCGCTAAAGGATGT
GAGCGGTGCAGTTATCAGGGTTATCGTGGGCGCGCCGGTATTTATCAGGTCGTGCCGATTTCTGATGAATTGGCGGAGCT
GATCTTGCATAACGCCTCAGCGAGTGATATTGAAGAGCAGTGTCAGAAAGAAGGCCATTGGGATTTGCGTCAGTCGGCGC
TCAATAAAGTTAAGCAGGGGATCACCAGTATTGAAGAAATACTGCGGGTCACCAGCGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-134)

Type II/IV secretion system protein (T2SSE)

(176-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

49.911

100

0.506
  pilB Acinetobacter baylyi ADP1

49.195

100

0.497
  pilB Legionella pneumophila strain ERS1305867

49.091

99.458

0.488
  pilF Neisseria gonorrhoeae MS11

48.294

100

0.486
  pilB Vibrio campbellii strain DS40M4

46.931

100

0.47
  pilB Vibrio cholerae strain A1552

46.909

99.458

0.467
  pilB Vibrio parahaemolyticus RIMD 2210633

46.618

98.915

0.461
  pilF Thermus thermophilus HB27

37.234

100

0.38