Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   L0U97_RS01765 Genome accession   NZ_CP090890
Coordinates   371463..373187 (-) Length   574 a.a.
NCBI ID   WP_234856793.1    Uniprot ID   -
Organism   Acinetobacter junii strain WCO-9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 366463..378187
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  L0U97_RS01735 - 366592..366918 (+) 327 WP_004958605.1 pyrimidine/purine nucleoside phosphorylase -
  L0U97_RS01740 rlmB 367047..367796 (+) 750 WP_004916859.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  L0U97_RS01745 - 367827..368738 (+) 912 WP_234856790.1 DMT family transporter -
  L0U97_RS01750 coaE 368735..369334 (-) 600 WP_234856791.1 dephospho-CoA kinase -
  L0U97_RS01755 pilD 369336..370196 (-) 861 WP_234856792.1 prepilin peptidase Machinery gene
  L0U97_RS01760 pilC 370209..371438 (-) 1230 WP_151690733.1 type II secretion system F family protein Machinery gene
  L0U97_RS01765 pilB 371463..373187 (-) 1725 WP_234856793.1 type IV-A pilus assembly ATPase PilB Machinery gene
  L0U97_RS01770 tpiA 373533..374324 (+) 792 WP_234856794.1 triose-phosphate isomerase -
  L0U97_RS01775 secG 374337..374666 (+) 330 WP_004916847.1 preprotein translocase subunit SecG -
  L0U97_RS01795 rimP 375289..375813 (+) 525 WP_004916844.1 ribosome maturation factor RimP -
  L0U97_RS01800 nusA 375851..377335 (+) 1485 WP_004916840.1 transcription termination factor NusA -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63678.84 Da        Isoelectric Point: 5.0686

>NTDB_id=646189 L0U97_RS01765 WP_234856793.1 371463..373187(-) (pilB) [Acinetobacter junii strain WCO-9]
MSGIHTSPKFSGFIRRIVDDGHIAASNMQSAIDAAKKAQQDIVAHLIEHHRLSPLTIAESISLEFGEPLFDLAVFDTALL
PREITDSKLIQKHRVVPLIQRGQILYVATSNPTNIDALDAIRFNTKLNIEPVIVEHNKLEKLIDQQFGDSGSFDFGENDD
FDLDNVDVATTDTNNTTEDEDTNNQEESPIVKYINKLLIDAIRMGASDLHFEPYEKMYRVRYRVDGVLRQIANPPLQLAT
RLASRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKELFMSA
LEKPQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNPKTGLTFSAALKAFLRQDPDVIMV
GEIRDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSECKQPIEIPKQSLL
EMGFTEQDLANPDFQIFQPVGCDACREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQ
GITSLQEVNRVTSE

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=646189 L0U97_RS01765 WP_234856793.1 371463..373187(-) (pilB) [Acinetobacter junii strain WCO-9]
ATGTCAGGAATACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGCATAGTCGATGATGGTCACATTGCTGCATCAAA
TATGCAGAGTGCGATTGATGCTGCAAAAAAAGCACAACAAGATATTGTTGCTCACTTAATTGAACATCATCGTCTATCAC
CTCTGACAATTGCGGAAAGCATATCTTTAGAGTTTGGTGAGCCACTCTTTGATCTTGCTGTATTTGATACAGCTCTATTA
CCTCGTGAAATAACTGACAGCAAACTGATTCAAAAACATCGTGTTGTTCCACTCATTCAACGTGGTCAGATTTTGTATGT
TGCGACCAGCAACCCGACGAATATTGATGCATTAGACGCAATCAGGTTCAATACCAAATTGAATATCGAACCTGTGATTG
TTGAGCACAATAAACTCGAAAAACTGATTGATCAGCAATTTGGAGATAGCGGCAGTTTTGATTTTGGGGAAAATGACGAC
TTTGACTTAGATAATGTTGATGTTGCAACGACTGATACAAACAACACGACTGAGGATGAGGATACAAATAATCAAGAAGA
ATCTCCTATCGTAAAATATATCAACAAGTTATTGATTGATGCGATTCGCATGGGAGCCTCTGACTTACATTTTGAGCCTT
ATGAAAAAATGTATCGCGTGCGTTATCGTGTGGATGGTGTACTCAGACAAATTGCTAACCCACCATTACAACTCGCAACT
CGTTTAGCCTCGCGCTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAGCGTATGCCTCAAGATGGTCGTATCAAACT
AAAACTCTCTAAAACTAAAGCGATTGATTTTCGTGTTAACTCACTTCCAACTTTATTTGGAGAGAAACTGGTTCTTCGTA
TCTTAGATCCTTCAAGCGCAATGTTAGGTATTGAAGCATTAGGCTATGAGGATGAACAAAAAGAACTCTTTATGAGTGCA
TTAGAAAAGCCTCAGGGTATGCTTCTGATTACAGGTCCAACAGGCTCAGGGAAAACTGTATCGTTGTATACAGGTTTAAA
TATCTTAAATACAGAAGATACCAATATTTCGACCGCAGAAGATCCCGTCGAAATTAACTTAGAAGGTATTAACCAAGTTA
ACGTCAACCCTAAAACAGGTTTAACTTTCTCAGCAGCATTAAAAGCTTTTTTACGTCAAGATCCTGATGTCATCATGGTC
GGTGAGATTCGTGATTTGGAAACAGCTGAAATTGCAATTAAGGCAGCTCAAACAGGTCACATGGTGATGTCGACCCTGCA
TACCAACAGCGCTCCTGAAACACTCACACGATTACGCAATATGGGGGTTCCATCTTTCAATATTGCAACTTCTGTTAATC
TAGTGATTGCTCAACGTTTAGCTCGTCGTCTATGCTCGGAATGCAAACAACCTATTGAGATTCCCAAGCAAAGCCTTTTA
GAAATGGGCTTCACTGAACAAGATTTAGCCAACCCTGACTTTCAAATTTTCCAACCTGTCGGCTGCGATGCTTGTCGTGA
AGGATATAAAGGGCGTGTCGGTATTTATGAGGTCATGAAAGTGACACCCGAGATTTCCAAGATTATTATGGAAGATGGAA
ATGCATTACAAATTGCAGCAGCATCTGAAAAGGCTGGTTTTAATAATCTACGCCGTTCTGGTTTAAAAAAGGTCATGCAA
GGGATCACCTCTTTGCAAGAAGTTAACCGAGTAACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

81.424

100

0.817
  pilB Acinetobacter baylyi ADP1

76.87

100

0.77
  pilB Legionella pneumophila strain ERS1305867

52.974

93.728

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

50

97.213

0.486
  pilF Neisseria gonorrhoeae MS11

50.457

95.296

0.481
  pilB Vibrio cholerae strain A1552

48.917

96.516

0.472
  pilB Vibrio campbellii strain DS40M4

51.451

90.07

0.463
  pilB/pilB1 Synechocystis sp. PCC 6803

34.74

100

0.373